Reviewed, UniProtKB/Swiss-Prot Q95QT2 (GALC_CAEEL)

Names and origin

Protein names

Recommended name:
    Putative galactocerebrosidase
      Short name=GALCERase
    EC=3.2.1.46
Alternative name(s):
    Galactosylceramidase
    Galactosylceramide beta-galactosidase
    Galactocerebroside beta-galactosidase

Gene names

ORF Names:

C29E4.10

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride By similarity.
Catalytic activity	D-galactosyl-N-acylsphingosine + H(2)O = D-galactose + N-acylsphingosine.
Sequence similarities	Belongs to the glycosyl hydrolase 59 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro galactosylceramide catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro galactosylceramidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 556	540	Putative galactocerebrosidase		PRO_0000012233
Amino acid modifications
	Glycosylation	141	1	N-linked (GlcNAc...) Potential
	Glycosylation	174	1	N-linked (GlcNAc...) Potential
	Glycosylation	193	1	N-linked (GlcNAc...) Potential
	Glycosylation	306	1	N-linked (GlcNAc...) Potential
	Glycosylation	322	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q95QT2-1 [UniParc]. Last modified April 29, 2008. Version 2. Checksum: BE955A3E6068CDE9	FASTA	556	63,559
	10 20 30 40 50 60 MFSIFIKIIL ILPSIATKTN NIDIKSWRSA QTFDGFGAVS GGGATSKLLF TYFSEKSDRK 70 80 90 100 110 120 QVLESLFNKD NGLQLLKVEM GGDDQSTEGT ESSHESEKGK ISKNNYEFQL ISEVREINPT 130 140 150 160 170 180 IPICVLPWAF PGWIGNTPYD NVTETAEYVV NWLKIGRDTW NFDTFCVGVW NERNFSESYV 190 200 210 220 230 240 KELRKILNLN GFNETLIVAG EGFRMDDSYS RLLDKRFINE YDIIGYEENL VISFSAFQST 250 260 270 280 290 300 QKYITSHVKR GWKILRESSG RFDGGGTYVT YTNGKDSTIF VETMSYKKSL CEYSSPRPYH 310 320 330 340 350 360 VKPRLNMSLN FGPSKFFSKS VNSTITILLP VNSFGILTTL PVSTPKRVTI KTPLVPLNYH 370 380 390 400 410 420 DDFENYEFDE EPKFWMPQKG SWVVRNGRAV QKVTRPSISW CTSHIRTPYA VMAYRKKNSI 430 440 450 460 470 480 LNAEVNIPKH STAKSIILGI RSNCSGCDIE VINCRGIFVE IEFSNKKVTI FSDFVDRSII 490 500 510 520 530 540 AEFKARRKVE FGKFYKFSIH LLDSHLFIKF GNHHVMTSVE IPEEQLNKTN NDTLFVIGTG 550 NFGISEWDNI STDYFH

References

[1]

"2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans." Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L. , Gardner A., Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M., Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C., Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A., Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M., Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K., Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L., Wilkinson-Sproat J., Wohldman P. Nature 368:32-38(1994) [PubMed: 7906398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

[2]

"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L23651 Genomic DNA. Translation: AAK84495.2.
RefSeq	NP_498726.2.
UniGene	Cel.25944
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	C29E4.10. Caenorhabditis elegans. [Contig view]
GeneID	183002.
NMPDR	fig|6239.3.peg.10673.
Organism-specific databases
WormBase	WBGene00016207. C29E4.10.
WormPep	C29E4.10. CE41985. [WorfDB]
Gene expression databases
ArrayExpress	Q95QT2.
Family and domain databases
InterPro	IPR001286. Glyco_hydro_59. IPR013781. Glyco_hydro_sub_cat. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF02057. Glyco_hydro_59. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	919578.

Entry information

Entry name

GALC_CAEEL

Accession

Primary (citable) accession number: Q95QT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	April 29, 2008
Last modified:	November 25, 2008
This is version 38 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project