Reviewed, UniProtKB/Swiss-Prot Q96LR9 (APLD1_HUMAN)

Names and origin

Protein names

Recommended name:
    Apolipoprotein-L domain-containing protein 1
Alternative name(s):
    Vascular early response gene protein

Gene names

Name:	APOLD1
Synonyms:	VERGE

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May be involved in angiogenesis. May play a role in activity-dependent changes of brain vasculature. May affect blood-brain permeability.
Subcellular location	Cell membrane; Multi-pass membrane proteinProbable.
Tissue specificity	Expressed in neonatal dermal microvascular endothelial cells.
Induction	In neonatal dermal microvascular endothelial cells, by hypoxia.
Sequence similarities	Belongs to the apolipoprotein L family.

Ontologies

Keywords
Biological process	Angiogenesis Differentiation
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Transmembrane
Molecular function	Developmental protein
Gene Ontology (GO)
None. [Check GOA]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96LR9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Notes: No experimental confirmation available.

Isoform 2 (identifier: Q96LR9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     2-32: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 279	279	Apolipoprotein-L domain-containing protein 1
Regions
	Transmembrane	83 – 105	23	Potential
	Transmembrane	122 – 142	21	Potential
	Transmembrane	192 – 212	21	Potential
	Coiled coil	226 – 253	28	Potential
	Compositional bias	3 – 82	80	Arg-rich
Natural variations
	Alternative sequence	2 – 32	31	Missing in isoform 2.
Experimental info
	Sequence conflict	64	1	G → R in CAB66717. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 25, 2006. Version 2. Checksum: E2CDA210C1FD80C3	FASTA	279	30,546
	10 20 30 40 50 60 MFRAPCHRLR ARGTRKARAG AWRGCTFPCL GKGMERPAAR EPHGPDALRR FQGLLLDRRG 70 80 90 100 110 120 RLHGQVLRLR EVARRLERLR RRSLVANVAG SSLSATGALA AIVGLSLSPV TLGTSLLVSA 130 140 150 160 170 180 VGLGVATAGG AVTITSDLSL IFCNSRELRR VQEIAATCQD QMREILSCLE FFCRWQGCGD 190 200 210 220 230 240 RQLLQCGRNA SIALYNSVYF IVFFGSRGFL IPRRAEGDTK VSQAVLKAKI QKLAESLESC 250 260 270 TGALDELSEQ LESRVQLCTK SSRGHDLKIS ADQRAGLFF
	Isoform 2 [UniParc]. Checksum: 291DE656CDC04338		248	27,063
	10 20 30 40 50 60 MGMERPAARE PHGPDALRRF QGLLLDRRGR LHGQVLRLRE VARRLERLRR RSLVANVAGS 70 80 90 100 110 120 SLSATGALAA IVGLSLSPVT LGTSLLVSAV GLGVATAGGA VTITSDLSLI FCNSRELRRV 130 140 150 160 170 180 QEIAATCQDQ MREILSCLEF FCRWQGCGDR QLLQCGRNAS IALYNSVYFI VFFGSRGFLI 190 200 210 220 230 240 PRRAEGDTKV SQAVLKAKIQ KLAESLESCT GALDELSEQL ESRVQLCTKS SRGHDLKISA DQRAGLFF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Testis.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Eye.
[4]	"Verge: a novel vascular early response gene." Regard J.B., Scheek S., Borbiev T., Lanahan A.A., Schneider A., Demetriades A.-M., Hiemisch H., Barnes C.A., Verin A.D., Worley P.F. J. Neurosci. 24:4092-4103(2004) [PubMed: 15102925] [Abstract] Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL136783 mRNA. Translation: CAB66717.1. AK057867 mRNA. Translation: BAB71601.1. BC042478 mRNA. Translation: AAH42478.1. Different initiation.
RefSeq	NP_001123887.1.
UniGene	Hs.23388
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSG00000178878. Homo sapiens. [Contig view]
GeneID	81575.
Organism-specific databases
HGNC	HGNC:25268. APOLD1.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	Q96LR9.
Gene expression databases
ArrayExpress	Q96LR9.
GermOnline	ENSG00000178878. Homo sapiens.
Family and domain databases
InterPro	IPR008405. ApoL. [Graphical view]
PANTHER	PTHR14096. ApoL. 1 hit.
Pfam	PF05461. ApoL. 1 hit. [Graphical view]
ProDom	Q96LR9. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

APLD1_HUMAN

Accession

Primary (citable) accession number: Q96LR9
Secondary accession number(s): Q8IVR2, Q9H0I5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 25, 2006
Last modified:	September 23, 2008
This is version 35 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)