Reviewed, UniProtKB/Swiss-Prot Q96M93 (ADAD1_HUMAN)

Names and origin

Protein names

Recommended name:
    Adenosine deaminase domain-containing protein 1
Alternative name(s):
    Testis nuclear RNA-binding protein

Gene names

Name:	ADAD1
Synonyms:	TENR

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plays a role in spermatogenesis. Binds to RNA but not to DNA By similarity.
Subcellular location	NucleusBy similarity.
Sequence similarities	Belongs to the ADAD family. Contains 1 A to I editase domain. Contains 1 DRBM (double-stranded RNA-binding) domain.

Ontologies

Keywords
Biological process	Differentiation Spermatogenesis
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	RNA-binding
Molecular function	Developmental protein
PTM	Phosphoprotein
Gene Ontology (GO)
None. [Check GOA]

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q96M93-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q96M93-2)

The sequence of this isoform differs from the canonical sequence as follows:
     283-293: Missing.

Isoform 3 (identifier: Q96M93-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: MASNNHWFQSSQVPSFAQM → M

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 576	576	Adenosine deaminase domain-containing protein 1
Regions
	Domain	96 – 164	69	DRBM
	Domain	249 – 574	326	A to I editase
Amino acid modifications
	Modified residue	227	1	Phosphotyrosine
	Modified residue	230	1	Phosphotyrosine
Natural variations
	Alternative sequence	1 – 19	19	MASNN…SFAQM → M in isoform 3.
	Alternative sequence	283 – 293	11	Missing in isoform 2.
Experimental info
	Sequence conflict	198	1	H → Y in BAC04125. Ref.1
	Sequence conflict	407	1	Q → R in BAC04125. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 379A6849ABDEEFAB	FASTA	576	64,411
	10 20 30 40 50 60 MASNNHWFQS SQVPSFAQML KKNLPVQPAT KTITTPTGWS SESYGLSKMA SKVTQVTGNF 70 80 90 100 110 120 PEPLLSKNLS SISNPVLPPK KIPKEFIMKY KRGEINPVSA LHQFAQMQRV QLDLKETVTT 130 140 150 160 170 180 GNVMGPYFAF CAVVDGIQYK TGLGQNKKES RSNAAKLALD ELLQLDEPEP RILETSGPPP 190 200 210 220 230 240 FPAEPVVLSE LAYVSKVHYE GRHIQYAKIS QIVKERFNQL ISNRSEYLKY SSSLAAFIIE 250 260 270 280 290 300 RAGQHEVVAI GTGEYNYSQD IKPDGRVLHD THAVVTARRS LLRYFYRQLL LFYSKNPAMM 310 320 330 340 350 360 EKSIFCTEPT SNLLTLKQNI NICLYMNQLP KGSAQIKSQL RLNPHSISAF EANEELCLHV 370 380 390 400 410 420 AVEGKIYLTV YCPKDGVNRI SSMSSSDKLT RWEVLGVQGA LLSHFIQPVY ISSILIGDGN 430 440 450 460 470 480 CSDTRGLEIA IKQRVDDALT SKLPMFYLVN RPHISLVPSA YPLQMNLEYK FLSLNWAQGD 490 500 510 520 530 540 VSLEIVDGLS GKITESSPFK SGMSMASRLC KAAMLSRFNL LAKEAKKELL EAGTYHAAKC 550 560 570 MSASYQEAKC KLKSYLQQHG YGSWIVKSPC IEQFNM
	Isoform 2 [UniParc]. Checksum: CFF0F974AE08AFD8		565	62,847
	10 20 30 40 50 60 MASNNHWFQS SQVPSFAQML KKNLPVQPAT KTITTPTGWS SESYGLSKMA SKVTQVTGNF 70 80 90 100 110 120 PEPLLSKNLS SISNPVLPPK KIPKEFIMKY KRGEINPVSA LHQFAQMQRV QLDLKETVTT 130 140 150 160 170 180 GNVMGPYFAF CAVVDGIQYK TGLGQNKKES RSNAAKLALD ELLQLDEPEP RILETSGPPP 190 200 210 220 230 240 FPAEPVVLSE LAYVSKVHYE GRHIQYAKIS QIVKERFNQL ISNRSEYLKY SSSLAAFIIE 250 260 270 280 290 300 RAGQHEVVAI GTGEYNYSQD IKPDGRVLHD THAVVTARRS LLSKNPAMME KSIFCTEPTS 310 320 330 340 350 360 NLLTLKQNIN ICLYMNQLPK GSAQIKSQLR LNPHSISAFE ANEELCLHVA VEGKIYLTVY 370 380 390 400 410 420 CPKDGVNRIS SMSSSDKLTR WEVLGVQGAL LSHFIQPVYI SSILIGDGNC SDTRGLEIAI 430 440 450 460 470 480 KQRVDDALTS KLPMFYLVNR PHISLVPSAY PLQMNLEYKF LSLNWAQGDV SLEIVDGLSG 490 500 510 520 530 540 KITESSPFKS GMSMASRLCK AAMLSRFNLL AKEAKKELLE AGTYHAAKCM SASYQEAKCK 550 560 LKSYLQQHGY GSWIVKSPCI EQFNM
	Isoform 3 [UniParc]. Checksum: ABCF6E031C9300AF		558	62,363
	10 20 30 40 50 60 MLKKNLPVQP ATKTITTPTG WSSESYGLSK MASKVTQVTG NFPEPLLSKN LSSISNPVLP 70 80 90 100 110 120 PKKIPKEFIM KYKRGEINPV SALHQFAQMQ RVQLDLKETV TTGNVMGPYF AFCAVVDGIQ 130 140 150 160 170 180 YKTGLGQNKK ESRSNAAKLA LDELLQLDEP EPRILETSGP PPFPAEPVVL SELAYVSKVH 190 200 210 220 230 240 YEGRHIQYAK ISQIVKERFN QLISNRSEYL KYSSSLAAFI IERAGQHEVV AIGTGEYNYS 250 260 270 280 290 300 QDIKPDGRVL HDTHAVVTAR RSLLRYFYRQ LLLFYSKNPA MMEKSIFCTE PTSNLLTLKQ 310 320 330 340 350 360 NINICLYMNQ LPKGSAQIKS QLRLNPHSIS AFEANEELCL HVAVEGKIYL TVYCPKDGVN 370 380 390 400 410 420 RISSMSSSDK LTRWEVLGVQ GALLSHFIQP VYISSILIGD GNCSDTRGLE IAIKQRVDDA 430 440 450 460 470 480 LTSKLPMFYL VNRPHISLVP SAYPLQMNLE YKFLSLNWAQ GDVSLEIVDG LSGKITESSP 490 500 510 520 530 540 FKSGMSMASR LCKAAMLSRF NLLAKEAKKE LLEAGTYHAA KCMSASYQEA KCKLKSYLQQ 550 HGYGSWIVKS PCIEQFNM

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Testis.
[2]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[5]	"Tyrosine phosphorylated Par3 regulates epithelial tight junction assembly promoted by EGFR signaling." Wang Y., Du D., Fang L., Yang G., Zhang C., Zeng R., Ullrich A., Lottspeich F., Chen Z. EMBO J. 25:5058-5070(2006) [PubMed: 17053785] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-227 AND TYR-230, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK057303 mRNA. Translation: BAB71416.1. AK093297 mRNA. Translation: BAC04125.1. AC022489 Genomic DNA. No translation available. CH471056 Genomic DNA. Translation: EAX05228.1. CH471056 Genomic DNA. Translation: EAX05234.1. BC040229 mRNA. Translation: AAH40229.1.
RefSeq	NP_640336.1.
UniGene	Hs.518957
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q96M93.
Genome annotation databases
Ensembl	ENSG00000164113. Homo sapiens. [Contig view]
GeneID	132612.
KEGG	hsa:132612.
Organism-specific databases
HGNC	HGNC:30713. ADAD1.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	Q96M93.
Gene expression databases
ArrayExpress	Q96M93.
CleanEx	HS_ADAD1.
Family and domain databases
InterPro	IPR002466. A_deamin. IPR001159. Ds_RNA_bd. IPR014720. dsRNA-bd-like. [Graphical view]
Gene3D	G3DSA:3.30.160.20. dsRNA-bd-like. 1 hit.
Pfam	PF02137. A_deamin. 1 hit. PF00035. dsrm. 1 hit. [Graphical view]
SMART	SM00552. ADEAMc. 1 hit. SM00358. DSRM. 1 hit. [Graphical view]
PROSITE	PS50141. A_DEAMIN_EDITASE. 1 hit. PS50137. DS_RBD. 1 hit. [Graphical view]
ProDom	Q96M93. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

ADAD1_HUMAN

Accession

Primary (citable) accession number: Q96M93
Secondary accession number(s): A6NKN4, Q8IWG6, Q8NA06

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	December 1, 2001
Last modified:	July 22, 2008
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)