Reviewed, UniProtKB/Swiss-Prot Q96NU7 (HUTI_HUMAN)

Names and origin

Protein names

Recommended name:
    Probable imidazolonepropionase
    EC=3.5.2.7
Alternative name(s):
    Amidohydrolase domain-containing protein 1

Gene names

Name:	AMDHD1
ORF Names:	HMFT1272

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H(2)O = N-formimidoyl-L-glutamate + H(+).
Cofactor	Binds 1 zinc or iron ion per subunit By similarity.
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3.
Sequence similarities	Belongs to the hutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Coding sequence diversity	Polymorphism
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Gene Ontology (GO)
Cellular component	cytosol Ref.2 Inferred from Experiment. Source: Reactome
Molecular function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides Ref.2 Inferred from Experiment. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 426	426	Probable imidazolonepropionase
Sites
	Metal binding	87	1	Zinc or iron By similarity
	Metal binding	89	1	Zinc or iron By similarity
	Metal binding	260	1	Zinc or iron By similarity
	Metal binding	334	1	Zinc or iron By similarity
	Binding site	96	1	Substrate By similarity
	Binding site	109	1	Substrate By similarity
	Binding site	159	1	Substrate By similarity
	Binding site	192	1	Substrate By similarity
	Binding site	263	1	Substrate By similarity
Natural variations
	Natural variant	3	1	G → S: dbSNP rs7955450.
	Natural variant	360	1	P → H: dbSNP rs17024904.

Sequences

	Sequence		Length	Mass (Da)
	Q96NU7-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: CAE8E3114A8ADE1F	FASTA	426	46,713
	10 20 30 40 50 60 MAGGHSLLLE NAQQVVLVCA RGERFLARDA LRSLAVLEGA SLVVGKDGFI KAIGPADVIQ 70 80 90 100 110 120 RQFSGETFEE IIDCSGKCIL PGLVDAHTHP VWAGERVHEF AMKLAGATYM EIHQAGGGIH 130 140 150 160 170 180 FTVERTRQAT EEELFRSLQQ RLQCMMRAGT TLVECKSGYG LDLETELKML RVIERARREL 190 200 210 220 230 240 DIGISATYCG AHSVPKGKTA TEAADDIINN HLPKLKELGR NGEIHVDNID VFCEKGVFDL 250 260 270 280 290 300 DSTRRILQRG KDIGLQINFH GDELHPMKAA ELGAELGAQA ISHLEEVSDE GIVAMATARC 310 320 330 340 350 360 SAILLPTTAY MLRLKQPRAR KMLDEGVIVA LGSDFNPNAY CFSMPMVMHL ACVNMRMSMP 370 380 390 400 410 420 EALAAATINA AYALGKSHTH GSLEVGKQGD LIIINSSRWE HLIYQFGGHH ELIEYVIAKG KLIYKT

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Adrenal gland.
[2]	"Expression profiling and differential screening between hepatoblastomas and the corresponding normal livers: identification of high expression of the PLK1 oncogene as a poor-prognostic indicator of hepatoblastomas." Yamada S., Ohira M., Horie H., Ando K., Takayasu H., Suzuki Y., Sugano S., Hirata T., Goto T., Matsunaga T., Hiyama E., Hayashi Y., Ando H., Suita S., Kaneko M., Sasaki F., Hashizume K., Ohnuma N., Nakagawara A. Oncogene 23:5901-5911(2004) [PubMed: 15221005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Hepatoblastoma.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK054617 mRNA. Translation: BAB70775.1. AB075878 mRNA. Translation: BAD38660.1. Different initiation. BC029146 mRNA. Translation: AAH29146.1.
RefSeq	NP_689648.1.
UniGene	Hs.424907
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSG00000139344. Homo sapiens. [Contig view]
GeneID	144193.
KEGG	hsa:144193.
Organism-specific databases
HGNC	HGNC:28577. AMDHD1.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	Q96NU7.
Enzyme and pathway databases
Reactome	REACT_13. Metabolism of amino acids.
Gene expression databases
ArrayExpress	Q96NU7.
CleanEx	HS_AMDHD1.
Family and domain databases
InterPro	IPR006680. Amidohydro_1. IPR005920. HutI. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
ProDom	PD001248. Amidohydro_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01224. hutI. 1 hit.
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

HUTI_HUMAN

Accession

Primary (citable) accession number: Q96NU7
Secondary accession number(s): Q68CI8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	December 1, 2001
Last modified:	July 22, 2008
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)