Reviewed, UniProtKB/Swiss-Prot Q99027 (COMP_BACSU)

Names and origin

Protein names

Sensor protein comP
Also known as:
     EC 2.7.13.3

Gene names

Name:	comP
Ordered Locus Names:	BSU31690

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein existence

Evidence at transcript level.

General annotation (Comments)

Function	Sensor in the two-component regulatory system comP/comA involved in a major quorum response pathway that regulates the development of genetic competence. Plays a role in sporulation, at least partly interchangeable with that of spoIIJ.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Post-translational modification	Autophosphorylates on a histidine and transfers the phosphate group onto an aspartate in comA, thus activating it.
Miscellaneous	The DNA sequences encoding comQ, comX and the N-terminal two-thirds of comP show a striking polymorphism, which determines the specificity of the quorum-sensing system in the different pherotypes of Bacillus.
Sequence similarities	Contains 1 histidine kinase domain.

Ontologies

Keywords
Biological process	Competence Sporulation Two-component regulatory system
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 769	769	Sensor protein comP
Regions
	Topological domain	1 – 9	9	Cytoplasmic Potential
	Transmembrane	10 – 33	24	Potential
	Topological domain	34 – 113	80	Extracellular Potential
	Transmembrane	114 – 134	21	Potential
	Topological domain	135 – 144	10	Cytoplasmic Potential
	Transmembrane	145 – 167	23	Potential
	Topological domain	168 – 235	68	Extracellular Potential
	Transmembrane	236 – 257	22	Potential
	Topological domain	258 – 272	15	Cytoplasmic Potential
	Transmembrane	273 – 295	23	Potential
	Topological domain	296 – 299	4	Extracellular Potential
	Transmembrane	300 – 323	24	Potential
	Topological domain	324 – 337	14	Cytoplasmic Potential
	Transmembrane	338 – 357	20	Potential
	Topological domain	358 – 361	4	Extracellular Potential
	Transmembrane	362 – 383	22	Potential
	Topological domain	384 – 769	386	Cytoplasmic Potential
	Domain	571 – 769	199	Histidine kinase
Amino acid modifications
	Modified residue	456	1	Phosphohistidine; by autocatalysis By similarity
Experimental info
	Sequence conflict	604	1	S → C in CAA37957 and AAA22319. Ref.1
	Sequence conflict	610	1	D → Y in CAA37957 and AAA22319. Ref.1
	Sequence conflict	628	1	E → G in CAA37957 and AAA22319. Ref.1
	Sequence conflict	636 – 637	2	QL → PV in CAA37957 and AAA22319. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q99027-1 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: E5CEACCE278DE5B4	FASTA	769	89,317
	10 20 30 40 50 60 MKNLIKKFTI AVIVLSILYI SYTTYISMNG IIIGTKIHKN DKSQFMIEEI SESSYGQFVG 70 80 90 100 110 120 LRQADIILKI NKEKPSDKHL KWGYLSHINS LDILRSGKKI HLKDFDLVTL NRPYSFFLFV 130 140 150 160 170 180 LPLFFYFLSI ICIFYILKVN KKRRSFAAYI LILLLLDISI AYISAGGPFR GHIINRYINL 190 200 210 220 230 240 FTFISSPILY LQFIQRYLGE IGKTFLNRIS FLYIIPIFNL GIEFFQDYLQ VDIDFLATLN 250 260 270 280 290 300 LVSFATLTLF SFSAIYLHLN KYKYAEHSFI LKLLILTNTL SFAPFLIFFV LPIIFTGNYI 310 320 330 340 350 360 FPALASASLL VLIPFGLVYQ FVANKMFDIE FILGRMRYYA LLAMIPTLLI VGALVLFDVM 370 380 390 400 410 420 DIQMNPVRQT VFFFVVMFAV FYFKEVMDFK FRLKRFSEKF NYQDSIFKYT QLMRGVTSLQ 430 440 450 460 470 480 QVFKELKNTI LDVLLVSKAY TFEVTPDHKV IFLDKHEVGP DWNFYQEEFE NVTSEIGKII 490 500 510 520 530 540 EVNQGFLMKV GERGGSSYVL LCLSNINTPR LTRDEISWLK TLSFYTSVSM ENVLHIEELM 550 560 570 580 590 600 EHLKDLKQEG TNPIWLKKLM FAIEEKQRSG LARDLHDSVL QDLISLKRQC ELFLGDFKKD 610 620 630 640 650 660 DNPSREEVQD KLVQMNEQMS DVISMTRETC HELRPQLLYD LGLVKALSKL VAHEQERVPF 670 680 690 700 710 720 HIRLNTGRFT ASLDLDSQLN LYRIIQEFLS NAVKHSQATD VLIMLISIQN KIVLHYEDDG 730 740 750 760 VGFDQEKNTE HSMSMGLSGI KERVRALDGR LRIETSEGKG FKADIEIEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A Bacillus subtilis regulatory gene product for genetic competence and sporulation resembles sensor protein members of the bacterial two-component signal-transduction systems." Weinrauch Y., Penchev R., Dubnau E., Smith I., Dubnau D. Genes Dev. 4:860-872(1990) [PubMed: 2116363] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BD630.
[2]	"Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai)." Oudega B., Koningstein G., Rodrigues L., de Sales Ramon M., Hilbert H., Duesterhoeft A., Pohl T.M., Weitzenegger T. Microbiology 143:2769-2774(1997) [PubMed: 9274030] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"Sequence and properties of comQ, a new competence regulatory gene of Bacillus subtilis." Weinrauch Y., Msadek T., Kunst F., Dubnau D. J. Bacteriol. 173:5685-5693(1991) [PubMed: 1715859] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16. Strain: 168.
[5]	"Specificity and genetic polymorphism of the Bacillus competence quorum-sensing system." Tortosa P., Logsdon L., Kraigher B., Itoh Y., Mandic-Mulec I., Dubnau D. J. Bacteriol. 183:451-460(2001) [PubMed: 11133937] [Abstract] Cited for: POLYMORPHISM IN COMX; COMQ AND COMP.
[6]	"Conservation of genes and processes controlled by the quorum response in bacteria: characterization of genes controlled by the quorum-sensing transcription factor ComA in Bacillus subtilis." Comella N., Grossman A.D. Mol. Microbiol. 57:1159-1174(2005) [PubMed: 16091051] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X54010 mRNA. Translation: CAA37957.1. Z93932 Genomic DNA. Translation: CAB07903.1. Z99120 Genomic DNA. Translation: CAB15157.1. M22856 Genomic DNA. Translation: AAA22319.1. M71283 Genomic DNA. Translation: AAA22324.1.
PIR	A35848. B69604.
RefSeq	NP_391047.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	938866.
GenomeReviews	Gene locus BSU31690 in contig AL009126_GR.
KEGG	bsu:BSU31690.
NMPDR	fig|224308.1.peg.3172.
Organism-specific databases
SubtiList	BG10380. comP. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	Q99027.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU3164-MON.
Family and domain databases
InterPro	IPR003594. ATP_bd_ATPase. IPR011712. Sig_transdc_His_kin_sub3_dim/P. IPR005467. Sig_transdc_His_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF02518. HATPase_c. 1 hit. PF07730. HisKA_3. 1 hit. [Graphical view]
SMART	SM00387. HATPase_c. 1 hit. [Graphical view]
PROSITE	PS50109. HIS_KIN. 1 hit. [Graphical view]
ProDom	Q99027. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

COMP_BACSU

Accession

Primary (citable) accession number: Q99027
Secondary accession number(s): O05226

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	May 30, 2000
Last modified:	June 10, 2008
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)