Reviewed, UniProtKB/Swiss-Prot Q9A5C1 (SYA_CAUCR)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name(s)=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	CC_2529

Organism

Caulobacter crescentus (Caulobacter vibrioides) [Complete proteome] [HAMAP]

Taxonomic identifier

155892 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	880 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Domain	Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 880	880	Alanyl-tRNA synthetase
Regions
	Zinc finger	169 – 182	14	C2H2-type

Sequences

	Sequence		Length	Mass (Da)
	Q9A5C1-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: C35DBF34DAE43B63	FASTA	880	93,643
	10 20 30 40 50 60 MPSLNEIRST FLDYFGKADH AVTPSAPLVP QNDPTLLFVN AGMVPFKNVF TGAETPAHPR 70 80 90 100 110 120 AASSQKCVRA GGKHNDLDNV GYTARHHTFF EMLGNFSFGD YFKEQAILHA WTLLTAEFKL 130 140 150 160 170 180 PKDKLLVTVY HTDDEAADLW KKIAGLGDDR IIRIPTSDNF WSMGDTGPCG PCSEIFFDHG 190 200 210 220 230 240 PHIAGGPPGS PDEDGDRFIE IWNLVFMQFE QLAGGERISL PKPSIDTGMG LERIAAVLQG 250 260 270 280 290 300 QHDNYDIDLF KALIAASVEL TGVKAEGAQA PSHRVIADHL RSSSFLLADG VSPSNEGRGY 310 320 330 340 350 360 VLRRIMRRAM RHAYLLGASE PLMHRLAPTL VREMGQAYPE LRRAEALIVE TLRQEEERFR 370 380 390 400 410 420 TTLGRGMGLL DEATANLAAG GVLDGETAFK LYDTYGFPLD LTQDAVRAKG LTVDTDAFDA 430 440 450 460 470 480 AMQKQREMAR ANWAGSGQQG AAAAWFSIKE KAGATDFVGY ETTETTGVVK AIVVDGAEVE 490 500 510 520 530 540 SASAGQTVEV LLDRTSFYAE SGGQAGDTGV IEAHGRESRV LDTQKQAGEL HVHRVELASD 550 560 570 580 590 600 LKVGDQVVAS VDADRRNTTR SNHSAAHLVH AALHHVLGPH VAQKGQMVDG ERMRFDFSHG 610 620 630 640 650 660 GPLTAEELDR IEAEVNEVIR QNVPAETKEM APQEAIEAGA VALFGEKYGD SVRVLTLGKS 670 680 690 700 710 720 LADEAKAYSV ELCGGTHVAR TGDIALFKIV SEQGVAAGVR RIEALTGEAA RRFLLDQAGV 730 740 750 760 770 780 AKALADQFKT PVAEVATRVD ALIADRKRLE KELAEAKKQL ALGGGGAASG PEDVNGTALI 790 800 810 820 830 840 ARVLDGVGGK ELRGVAEEFK KQLASGVVAL VGTSDGKAAV TVAVTADLTG KFSAADLAKA 850 860 870 880 AVIAMGGQGA GGKADFAQGG APDDSKAEEG LAAVKALLAG

References

[1]

"Complete genome sequence of Caulobacter crescentus." Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E., Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R., Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B., DeBoy R.T., Dodson R.J. , Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R., Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O., Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001) [PubMed: 11259647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19089 / CB15.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005673 Genomic DNA. Translation: AAK24500.1.
PIR	H87562.
RefSeq	NP_421332.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	943927.
GenomeReviews	Gene locus CC_2529 in contig AE005673_GR.
KEGG	ccr:CC_2529.
NMPDR	fig|190650.1.peg.2512.
TIGR	CC_2529.
Phylogenomic databases
HOGENOM	Q9A5C1.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	Q9A5C1. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_CAUCR

Accession

Primary (citable) accession number: Q9A5C1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2002
Last sequence update:	June 1, 2001
Last modified:	July 22, 2008
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)