Reviewed, UniProtKB/Swiss-Prot Q9BRR6 (ADPGK_HUMAN)

Names and origin

Protein names

Recommended name:
    ADP-dependent glucokinase
      Short name=ADP-GK
      Short name=ADPGK
    EC=2.7.1.147
Alternative name(s):
    RbBP-35

Gene names

Name:	ADPGK
ORF Names:	PSEC0260

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	497 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency By similarity.
Catalytic activity	ADP + D-glucose = AMP + D-glucose 6-phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Carbohydrate degradation; glycolysis.
Subunit structure	Monomer By similarity.
Subcellular location	SecretedPotential.
Sequence similarities	Belongs to the ADP-dependent glucokinase family. Contains 1 ADPK (ADP-dependent kinase) domain.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Ligand	Magnesium Metal-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
None. [Check GOA]

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9BRR6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9BRR6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     313-313: Missing.

Isoform 3 (identifier: Q9BRR6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-239: Missing.

Isoform 4 (identifier: Q9BRR6-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-274: Missing.
     275-280: RKRLLE → MAASCR
     313-313: Missing.

Isoform 5 (identifier: Q9BRR6-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-122: Missing.
     404-469: AAGARVAGTQ...ISFHFTPVLV → LGSSCGWDTG...ILPLHTSIGV
     470-497: Missing.

Isoform 6 (identifier: Q9BRR6-6)

The sequence of this isoform differs from the canonical sequence as follows:
     79-497: Missing.

Notes: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 497	475	ADP-dependent glucokinase
Regions
	Domain	52 – 497	446	ADPK
Sites
	Active site	481	1	Proton acceptor By similarity
	Metal binding	297	1	Magnesium By similarity
	Metal binding	328	1	Magnesium By similarity
	Metal binding	481	1	Magnesium By similarity
Natural variations
	Alternative sequence	1 – 274	274	Missing in isoform 4.
	Alternative sequence	1 – 239	239	Missing in isoform 3.
	Alternative sequence	1 – 122	122	Missing in isoform 5.
	Alternative sequence	79 – 497	419	Missing in isoform 6.
	Alternative sequence	275 – 280	6	RKRLLE → MAASCR in isoform 4.
	Alternative sequence	313	1	Missing in isoform 2 and isoform 4.
	Alternative sequence	404 – 469	66	AAGAR…TPVLV → LGSSCGWDTGLRHRNHRHQP SVSEGTPRVHDFPFGGRLQD CIKPKQASSRMAQRGNILPL HTSIGV in isoform 5.
	Alternative sequence	470 – 497	28	Missing in isoform 5.

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: B758E977CDA88F8F	FASTA	497	54,089
	10 20 30 40 50 60 MALWRGSAYA GFLALAVGCV FLLEPELPGS ALRSLWSSLC LGPAPAPPGP VSPEGRLAAA 70 80 90 100 110 120 WDALIVRPVR RWRRVAVGVN ACVDVVLSGV KLLQALGLSP GNGKDHSILH SRNDLEEAFI 130 140 150 160 170 180 HFMGKGAAAE RFFSDKETFH DIAQVASEFP GAQHYVGGNA ALIGQKFAAN SDLKVLLCGP 190 200 210 220 230 240 VGPKLHELLD DNVFVPPESL QEVDEFHLIL EYQAGEEWGQ LKAPHANRFI FSHDLSNGAM 250 260 270 280 290 300 NMLEVFVSSL EEFQPDLVVL SGLHMMEGQS KELQRKRLLE VVTSISDIPT GIPVHLELAS 310 320 330 340 350 360 MTNRELMSSI VHQQVFPAVT SLGLNEQELL FLTQSASGPH SSLSSWNGVP DVGMVSDILF 370 380 390 400 410 420 WILKEHGRSK SRASDLTRIH FHTLVYHILA TVDGHWANQL AAVAAGARVA GTQACATETI 430 440 450 460 470 480 DTSRVSLRAP QEFMTSHSEA GSRIVLNPNK PVVEWHREGI SFHFTPVLVC KDPIRTVGLG 490 DAISAEGLFY SEVHPHY
	Isoform 2 [UniParc]. Checksum: 5ACBE523E6EE8828		496	53,961
	10 20 30 40 50 60 MALWRGSAYA GFLALAVGCV FLLEPELPGS ALRSLWSSLC LGPAPAPPGP VSPEGRLAAA 70 80 90 100 110 120 WDALIVRPVR RWRRVAVGVN ACVDVVLSGV KLLQALGLSP GNGKDHSILH SRNDLEEAFI 130 140 150 160 170 180 HFMGKGAAAE RFFSDKETFH DIAQVASEFP GAQHYVGGNA ALIGQKFAAN SDLKVLLCGP 190 200 210 220 230 240 VGPKLHELLD DNVFVPPESL QEVDEFHLIL EYQAGEEWGQ LKAPHANRFI FSHDLSNGAM 250 260 270 280 290 300 NMLEVFVSSL EEFQPDLVVL SGLHMMEGQS KELQRKRLLE VVTSISDIPT GIPVHLELAS 310 320 330 340 350 360 MTNRELMSSI VHQVFPAVTS LGLNEQELLF LTQSASGPHS SLSSWNGVPD VGMVSDILFW 370 380 390 400 410 420 ILKEHGRSKS RASDLTRIHF HTLVYHILAT VDGHWANQLA AVAAGARVAG TQACATETID 430 440 450 460 470 480 TSRVSLRAPQ EFMTSHSEAG SRIVLNPNKP VVEWHREGIS FHFTPVLVCK DPIRTVGLGD 490 AISAEGLFYS EVHPHY
	Isoform 3 [UniParc]. Checksum: 14A1E87AB18DDB1B		258	28,413
	10 20 30 40 50 60 MNMLEVFVSS LEEFQPDLVV LSGLHMMEGQ SKELQRKRLL EVVTSISDIP TGIPVHLELA 70 80 90 100 110 120 SMTNRELMSS IVHQQVFPAV TSLGLNEQEL LFLTQSASGP HSSLSSWNGV PDVGMVSDIL 130 140 150 160 170 180 FWILKEHGRS KSRASDLTRI HFHTLVYHIL ATVDGHWANQ LAAVAAGARV AGTQACATET 190 200 210 220 230 240 IDTSRVSLRA PQEFMTSHSE AGSRIVLNPN KPVVEWHREG ISFHFTPVLV CKDPIRTVGL 250 GDAISAEGLF YSEVHPHY
	Isoform 4 [UniParc]. Checksum: F387E8FABA303003		222	24,130
	10 20 30 40 50 60 MAASCRVVTS ISDIPTGIPV HLELASMTNR ELMSSIVHQV FPAVTSLGLN EQELLFLTQS 70 80 90 100 110 120 ASGPHSSLSS WNGVPDVGMV SDILFWILKE HGRSKSRASD LTRIHFHTLV YHILATVDGH 130 140 150 160 170 180 WANQLAAVAA GARVAGTQAC ATETIDTSRV SLRAPQEFMT SHSEAGSRIV LNPNKPVVEW 190 200 210 220 HREGISFHFT PVLVCKDPIR TVGLGDAISA EGLFYSEVHP HY
	Isoform 5 [UniParc]. Checksum: 6D5F512F495A60F6		347	38,254
	10 20 30 40 50 60 MGKGAAAERF FSDKETFHDI AQVASEFPGA QHYVGGNAAL IGQKFAANSD LKVLLCGPVG 70 80 90 100 110 120 PKLHELLDDN VFVPPESLQE VDEFHLILEY QAGEEWGQLK APHANRFIFS HDLSNGAMNM 130 140 150 160 170 180 LEVFVSSLEE FQPDLVVLSG LHMMEGQSKE LQRKRLLEVV TSISDIPTGI PVHLELASMT 190 200 210 220 230 240 NRELMSSIVH QQVFPAVTSL GLNEQELLFL TQSASGPHSS LSSWNGVPDV GMVSDILFWI 250 260 270 280 290 300 LKEHGRSKSR ASDLTRIHFH TLVYHILATV DGHWANQLAA VLGSSCGWDT GLRHRNHRHQ 310 320 330 340 PSVSEGTPRV HDFPFGGRLQ DCIKPKQASS RMAQRGNILP LHTSIGV
	Isoform 6. Checksum: CE817CAB68D45EB9		78	8,252
	10 20 30 40 50 60 MALWRGSAYA GFLALAVGCV FLLEPELPGS ALRSLWSSLC LGPAPAPPGP VSPEGRLAAA 70 WDALIVRPVR RWRRVAVG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"RbBP-35 interacted with pRb." Fan Z.S., Ao S.Z. Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5). Tissue: Lymph node.
[2]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Tissue: Testis.
[3]	"HRI human cDNA sequencing project." Ota T., Nishikawa T., Suzuki Y., Kawai-Hio Y., Hayashi K., Ishii S., Saito K., Yamamoto J., Wakamatsu A., Nagai T., Nakamura Y., Nagahari K., Sugano S., Isogai T. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Teratocarcinoma.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Heart.
[5]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 6). Tissue: Brain and Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF204270 mRNA. Translation: AAL31473.1. AL136873 mRNA. Translation: CAB66807.1. AK075560 mRNA. Translation: BAC11699.1. AK055526 mRNA. Translation: BAB70941.1. CR533447 mRNA. Translation: CAG38478.1. BC006112 mRNA. Translation: AAH06112.1. BC018074 mRNA. Translation: AAH18074.1.
UniGene	Hs.654636
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSG00000159322. Homo sapiens. [Contig view]
Organism-specific databases
HGNC	HGNC:25250. ADPGK.
MIM	611861. gene.
PharmGKB	PA134971928.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	Q9BRR6.
HOVERGEN	Q9BRR6.
Gene expression databases
ArrayExpress	Q9BRR6.
CleanEx	HS_ADPGK.
GermOnline	ENSG00000159322. Homo sapiens.
Family and domain databases
InterPro	IPR007666. ADP_PFK/GK. [Graphical view]
PANTHER	PTHR21208. ADP_PFK/GK. 1 hit.
Pfam	PF04587. ADP_PFK_GK. 1 hit. [Graphical view]
PROSITE	PS51255. ADPK. 1 hit. [Graphical view]
ProDom	Q9BRR6. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	Q9BRR6.
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

ADPGK_HUMAN

Accession

Primary (citable) accession number: Q9BRR6
Secondary accession number(s): Q49AU7 , Q8NBI1, Q8WZ90, Q96NF8, Q9H0A7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	June 1, 2001
Last modified:	September 2, 2008
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)