Reviewed, UniProtKB/Swiss-Prot Q9BXN1 (ASPN_HUMAN)

Names and origin

Protein names

Recommended name:
    Asporin
Alternative name(s):
    Periodontal ligament-associated protein 1
      Short name(s)=PLAP-1

Gene names

Name:	ASPN
Synonyms:	PLAP1, SLRR1C
ORF Names:	UNQ215/PRO241

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Secreted › extracellular space › extracellular matrix.
Tissue specificity	Higher levels in osteoarthritic articular cartilage, aorta, uterus. Moderate expression in small intestine, heart, liver, bladder, ovary, stomach, and in the adrenal, thyroid, and mammary glands. Low expression in trachea, bone marrow, and lung.
Post-translational modification	There is no serine/glycine dipeptide sequence expected for the attachment of O-linked glycosaminoglycans and this is probably not a proteoglycan. The O-linked polysaccharide on 54-Ser is probably the mucin type linked to GalNAc. The N-linked glycan at Asn-281 is composed of variable structures of GlcNAc, mannose, fucose, HexNAc and hexose.
Polymorphism	The poly-Asp region of ASPN is polymorphic and ranges at least from 11 to 17 Asp.
Sequence similarities	Belongs to the small leucine-rich proteoglycan (SLRP) family. Class I subfamily. Contains 12 LRR (leucine-rich) repeats.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Coding sequence diversity	Polymorphism Triplet repeat expansion
Domain	Leucine-rich repeat Repeat Signal
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 14	14	Potential
	Propeptide	15 – 32	18	Potential
	Chain	33 – 379	347	Asporin
Regions
	Repeat	95 – 115	21	LRR-S 1
	Repeat	116 – 139	24	LRR-T 1
	Repeat	140 – 163	24	LRR-T 2
	Repeat	164 – 184	21	LRR-S 2
	Repeat	185 – 208	24	LRR-T 3
	Repeat	209 – 233	25	LRR-T 4
	Repeat	234 – 254	21	LRR-S 3
	Repeat	255 – 278	24	LRR-T 5
	Repeat	279 – 302	24	LRR-T 6
	Repeat	303 – 325	23	LRR-S 4
	Repeat	326 – 355	30	LRR-T 7
	Repeat	356 – 379	24	LRR-T 8
	Compositional bias	36 – 52	17	Poly-Asp
	Compositional bias	76 – 89	14	Cys-rich
Amino acid modifications
	Glycosylation	54	1	O-linked (GalNAc...) Probable
	Glycosylation	281	1	N-linked (GlcNAc...)
	Disulfide bond	332 ↔ 365		By similarity
Experimental info
	Sequence conflict	237 – 242	6	GLPPTL → DNLPSF Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9BXN1-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 786625859E26A96D	FASTA	379	43,302
	10 20 30 40 50 60 MKEYVLLLFL ALCSAKPFFS PSHIALKNMM LKDMEDTDDD DDDDDDDDDD EDNSLFPTRE 70 80 90 100 110 120 PRSHFFPFDL FPMCPFGCQC YSRVVHCSDL GLTSVPTNIP FDTRMLDLQN NKIKEIKEND 130 140 150 160 170 180 FKGLTSLYGL ILNNNKLTKI HPKAFLTTKK LRRLYLSHNQ LSEIPLNLPK SLAELRIHEN 190 200 210 220 230 240 KVKKIQKDTF KGMNALHVLE MSANPLDNNG IEPGAFEGVT VFHIRIAEAK LTSVPKGLPP 250 260 270 280 290 300 TLLELHLDYN KISTVELEDF KRYKELQRLG LGNNKITDIE NGSLANIPRV REIHLENNKL 310 320 330 340 350 360 KKIPSGLPEL KYLQIIFLHS NSIARVGVND FCPTVPKMKK SLYSAISLFN NPVKYWEMQP 370 ATFRCVLSRM SVQLGNFGM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of asporin. A novel member of the leucine-rich repeat protein family closely related to decorin and biglycan." Lorenzo P., Aspberg A., Oennerfjord P., Bayliss M.T., Neame P.J., Heinegaard D. J. Biol. Chem. 276:12201-12211(2001) [PubMed: 11152692] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, GLYCOSYLATION AT SER-54 AND ASN-281, MASS SPECTROMETRY, POLYMORPHISM OF POLY-ASP REGION. Tissue: Cartilage.
[2]	"Expression profile of active genes in human periodontal ligament and isolation of PLAP-1, a novel SLRP family gene." Yamada S., Murakami S., Matoba R., Ozawa Y., Yokokoji T., Nakahira Y., Ikezawa K., Takayama S., Matsubara K., Okada H. Gene 275:279-286(2001) [PubMed: 11587855] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon and Embryo.
[4]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Expression pattern and gene characterization of asporin. A newly discovered member of the leucine-rich repeat protein family." Henry S.P., Takanosu M., Boyd T.C., Mayne P.M., Eberspaecher H., Zhou W., de Crombrugghe B., Hoeoek M., Mayne R. J. Biol. Chem. 276:12212-12221(2001) [PubMed: 11152695] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF316824 mRNA. Translation: AAK35161.1. AY029191 mRNA. Translation: AAK31800.1. AK000136 mRNA. Translation: BAA90967.1. Different initiation. AK027359 mRNA. Translation: BAB55060.1. AY358329 mRNA. Translation: AAQ88695.1.
RefSeq	NP_060150.3.
UniGene	Hs.435655
3D structure databases
HSSP	HSSP built from PDB template 1M0Z based on UniProtKB P07359.
ModBase	Search...
Genome annotation databases
Ensembl	ENSG00000106819. Homo sapiens. [Contig view]
GeneID	54829.
KEGG	hsa:54829.
Organism-specific databases
H-InvDB	HIX0008167.
HGNC	HGNC:14872. ASPN.
HPA	HPA008435.
MIM	608135. gene.
PharmGKB	PA25057.
GenAtlas	Search...
GeneCards	Search...
GeneLynx	Search...
Phylogenomic databases
HOVERGEN	Q9BXN1.
Gene expression databases
ArrayExpress	Q9BXN1.
CleanEx	HS_ASPN.
GermOnline	ENSG00000106819. Homo sapiens.
Family and domain databases
InterPro	IPR001611. LRR. IPR000372. LRR_cys_N. IPR016352. SLRP_I_decor/aspor/byglycan. [Graphical view]
Pfam	PF00560. LRR_1. 6 hits. PF01462. LRRNT. 1 hit. [Graphical view]
PIRSF	PIRSF002490. SLRP_I. 1 hit.
PRINTS	PR00019. LEURICHRPT.
SMART	SM00013. LRRNT. 1 hit. [Graphical view]
ProDom	Q9BXN1. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

ASPN_HUMAN

Accession

Primary (citable) accession number: Q9BXN1
Secondary accession number(s): Q96K79, Q96LD0, Q9NXP3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2002
Last sequence update:	June 1, 2001
Last modified:	July 22, 2008
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)