Reviewed, UniProtKB/Swiss-Prot Q9C0K7 (STRAB_HUMAN)

Names and origin

Protein names

Recommended name:
    STE20-related kinase adapter protein beta
      Short name=STRAD beta
Alternative name(s):
    Pseudokinase ALS2CR2
    Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 2 protein
    ILP-interacting protein
    CALS-21

Gene names

Name:	STRADB
Synonyms:	ALS2CR2, ILPIP
ORF Names:	PRO1038

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Pseudokinase which, in complex with CAB39, binds to and activates STK11. Relocates STK11 from the nucleus to the cytoplasm. Plays an essential role in STK11-mediated G1 cell cycle arrest.
Subunit structure	Interacts with BIRC4/XIAP. These two proteins are likely to coexist in a complex with TAK1, TRAF6, TAB1 and TAB2.
Subcellular location	Nucleus. Cytoplasm.
Tissue specificity	Highly expressed in heart, skeletal muscle, testis, liver and colon.
Domain	The protein kinase domain is predicted to be catalytically inactive.
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 protein kinase domain.
Caution	Ser-184 is present instead of the conserved Asp which is expected to be an active site residue.

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding
Gene Ontology (GO)
Biological process	activation of protein kinase activity Ref.7 Inferred from direct assay. Source: UniProtKB cell cycle Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Ref.7 Inferred from electronic annotation. Source: InterPro protein export from nucleus Ref.7 Inferred from direct assay. Source: UniProtKB
Cellular component	cytoplasm Ref.7 Inferred from direct assay. Source: UniProtKB nucleus Ref.7 Inferred from direct assay. Source: UniProtKB
Molecular function	ATP binding Non-traceable author statement. Source: UniProtKB protein binding Inferred from physical interaction. Source: IntAct protein kinase activity Ref.7 Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9C0K7-1)

Also known as: ILPIP-alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9C0K7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     372-377: MKEESQ → PYFEFL
     378-418: Missing.

Notes: No experimental confirmation available.

Isoform 3 (identifier: Q9C0K7-3)

Also known as: ILPIP-beta;

The sequence of this isoform differs from the canonical sequence as follows:
     1-138: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 418	418	STE20-related kinase adapter protein beta		PRO_0000085617
Regions
	Domain	58 – 369	312	Protein kinase
	Nucleotide binding	64 – 72	9	ATP By similarity
Sites
	Binding site	89	1	ATP By similarity
Natural variations
	Alternative sequence	1 – 138	138	Missing in isoform 3.		VSP_016625
	Alternative sequence	372 – 377	6	MKEESQ → PYFEFL in isoform 2.		VSP_016623
	Alternative sequence	378 – 418	41	Missing in isoform 2.		VSP_016624
	Natural variant	155	1	G → E in a metastatic melanoma sample; somatic mutation.		VAR_041335
	Natural variant	386	1	P → L		VAR_041336

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (ILPIP-alpha) [UniParc]. Last modified June 1, 2001. Version 1. Checksum: A63ED6CCE1E18C96	FASTA	418	47,026
	10 20 30 40 50 60 MSLLDCFCTS RTQVESLRPE KQSETSIHQY LVDEPTLSWS RPSTRASEVL CSTNVSHYEL 70 80 90 100 110 120 QVEIGRGFDN LTSVHLARHT PTGTLVTIKI TNLENCNEER LKALQKAVIL SHFFRHPNIT 130 140 150 160 170 180 TYWTVFTVGS WLWVISPFMA YGSASQLLRT YFPEGMSETL IRNILFGAVR GLNYLHQNGC 190 200 210 220 230 240 IHRSIKASHI LISGDGLVTL SGLSHLHSLV KHGQRHRAVY DFPQFSTSVQ PWLSPELLRQ 250 260 270 280 290 300 DLHGYNVKSD IYSVGITACE LASGQVPFQD MHRTQMLLQK LKGPPYSPLD ISIFPQSESR 310 320 330 340 350 360 MKNSQSGVDS GIGESVLVSS GTHTVNSDRL HTPSSKTFSP AFFSLVQLCL QQDPEKRPSA 370 380 390 400 410 SSLLSHVFFK QMKEESQDSI LSLLPPAYNK PSISLPPVLP WTEPECDFPD EKDSYWEF
	Isoform 2 [UniParc]. Checksum: C869F81FDED0839A		377	42,371
	10 20 30 40 50 60 MSLLDCFCTS RTQVESLRPE KQSETSIHQY LVDEPTLSWS RPSTRASEVL CSTNVSHYEL 70 80 90 100 110 120 QVEIGRGFDN LTSVHLARHT PTGTLVTIKI TNLENCNEER LKALQKAVIL SHFFRHPNIT 130 140 150 160 170 180 TYWTVFTVGS WLWVISPFMA YGSASQLLRT YFPEGMSETL IRNILFGAVR GLNYLHQNGC 190 200 210 220 230 240 IHRSIKASHI LISGDGLVTL SGLSHLHSLV KHGQRHRAVY DFPQFSTSVQ PWLSPELLRQ 250 260 270 280 290 300 DLHGYNVKSD IYSVGITACE LASGQVPFQD MHRTQMLLQK LKGPPYSPLD ISIFPQSESR 310 320 330 340 350 360 MKNSQSGVDS GIGESVLVSS GTHTVNSDRL HTPSSKTFSP AFFSLVQLCL QQDPEKRPSA 370 SSLLSHVFFK QPYFEFL
	Isoform 3 (ILPIP-beta) [UniParc]. Checksum: 9D433EAA77D5CF08		280	31,219
	10 20 30 40 50 60 MAYGSASQLL RTYFPEGMSE TLIRNILFGA VRGLNYLHQN GCIHRSIKAS HILISGDGLV 70 80 90 100 110 120 TLSGLSHLHS LVKHGQRHRA VYDFPQFSTS VQPWLSPELL RQDLHGYNVK SDIYSVGITA 130 140 150 160 170 180 CELASGQVPF QDMHRTQMLL QKLKGPPYSP LDISIFPQSE SRMKNSQSGV DSGIGESVLV 190 200 210 220 230 240 SSGTHTVNSD RLHTPSSKTF SPAFFSLVQL CLQQDPEKRP SASSLLSHVF FKQMKEESQD 250 260 270 280 SILSLLPPAY NKPSISLPPV LPWTEPECDF PDEKDSYWEF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of three novel genes, ALS2CR1, ALS2CR2, and ALS2CR3, in the juvenile amyotrophic lateral sclerosis (ALS2) critical region at chromosome 2q33-q34: candidate genes for ALS2." Hadano S., Yanagisawa Y., Skaug J., Fichter K., Nasir J., Martindale D., Koop B.F., Scherer S.W., Nicholson D.W., Rouleau G.A., Ikeda J.-E., Hayden M.R. Genomics 71:200-213(2001) [PubMed: 11161814] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]	"ILPIP, a novel anti-apoptotic protein that enhances XIAP-mediated activation of JNK1 and protection against apoptosis." Sanna M.G., Da Silva Correia J., Luo Y., Chuang B., Paulson L.M., Nguyen B., Deveraux Q.L., Ulevitch R.J. J. Biol. Chem. 277:30454-30462(2002) [PubMed: 12048196] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), TISSUE SPECIFICITY, INTERACTION WITH BIRC4.
[3]	"Functional prediction of the coding sequences of 121 new genes deduced by analysis of cDNA clones from human fetal liver." Zhang C., Yu Y., Zhang S., Wei H., Zhou G., Ouyang S., Luo L., Bi J., Liu M., He F. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Fetal liver.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Placenta.
[7]	"MO25alpha/beta interact with STRADalpha/beta enhancing their ability to bind, activate and localize LKB1 in the cytoplasm." Boudeau J., Baas A.F., Deak M., Morrice N.A., Kieloch A., Schutkowski M., Prescott A.R., Clevers H.C., Alessi D.R. EMBO J. 22:5102-5114(2003) [PubMed: 14517248] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH STK11 AND CAB39.
[8]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] GLU-155 AND LEU-386.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB038950 mRNA. Translation: BAB32500.1. AY093697 mRNA. Translation: AAM19143.1. AF116618 mRNA. Translation: AAF71042.1. AK027637 mRNA. Translation: BAB55254.1. AC007282 Genomic DNA. Translation: AAY14692.1. BC008302 mRNA. Translation: AAH08302.1. BC090871 mRNA. Translation: AAH90871.1.
RefSeq	NP_061041.2.
UniGene	Hs.652338
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9C0K7.
PTM databases
PhosphoSite	Q9C0K7.
Genome annotation databases
Ensembl	ENSG00000082146. Homo sapiens. [Contig view]
GeneID	55437.
KEGG	hsa:55437.
Organism-specific databases
H-InvDB	HIX0002739. HIX0029700.
HGNC	HGNC:13205. STRADB.
MIM	607333. gene.
PharmGKB	PA24743.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	Q9C0K7.
HOVERGEN	Q9C0K7.
Gene expression databases
CleanEx	HS_ALS2CR2.
GermOnline	ENSG00000082146. Homo sapiens.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	Q9C0K7.
NextBio	59838.
SOURCE	Search...

Entry information

Entry name

STRAB_HUMAN

Accession

Primary (citable) accession number: Q9C0K7
Secondary accession number(s): Q5BKY7, Q9P1L0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	June 1, 2001
Last modified:	November 25, 2008
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)