Reviewed, UniProtKB/Swiss-Prot Q9C0V1 (AMT1_SCHPO)

Names and origin

Protein names

Recommended name:
    Ammonium transporter 1

Gene names

Name:	amt1
ORF Names:	SPCPB1C11.01

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	497 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Transporter for ammonium to use as a nitrogen source. Under ammonium limitation acts as an ammonium sensor, generating a signal that leads to pseudohyphal growth.
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the ammonium transporter (TC 2.A.49) family. [View classification]

Ontologies

Keywords
Biological process	Ammonia transport Transport
Cellular component	Membrane
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ammonium transport Ref.2 Inferred from electronic annotation. Source: UniProtKB-KW invasive growth in response to glucose limitation Ref.2 Inferred from mutant phenotype. Source: GeneDB_SPombe methylammonium transport Ref.2 Inferred from mutant phenotype. Source: GeneDB_SPombe response to nutrient Ref.2 Inferred from mutant phenotype. Source: GeneDB_SPombe
Cellular component	Golgi apparatus Ref.3 Inferred from direct assay. Source: GeneDB_SPombe integral to plasma membrane Ref.2 Traceable author statement. Source: GeneDB_SPombe
Molecular function	high affinity secondary active ammonium transmembrane transporter activity Ref.2 Inferred from mutant phenotype. Source: GeneDB_SPombe methylammonium transmembrane transporter activity Ref.2 Inferred from mutant phenotype. Source: GeneDB_SPombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 497	497	Ammonium transporter 1		PRO_0000278387
Regions
	Topological domain	1 – 32	32	Extracellular Potential
	Transmembrane	33 – 53	21	Potential
	Topological domain	54 – 63	10	Cytoplasmic Potential
	Transmembrane	64 – 84	21	Potential
	Topological domain	85 – 122	38	Extracellular Potential
	Transmembrane	123 – 143	21	Potential
	Topological domain	144 – 150	7	Cytoplasmic Potential
	Transmembrane	151 – 171	21	Potential
	Topological domain	172 – 187	16	Extracellular Potential
	Transmembrane	188 – 208	21	Potential
	Topological domain	209 – 223	15	Cytoplasmic Potential
	Transmembrane	224 – 244	21	Potential
	Topological domain	245 – 253	9	Extracellular Potential
	Transmembrane	254 – 274	21	Potential
	Topological domain	275 – 281	7	Cytoplasmic Potential
	Transmembrane	282 – 302	21	Potential
	Topological domain	303	1	Extracellular Potential
	Transmembrane	304 – 324	21	Potential
	Topological domain	325 – 338	14	Cytoplasmic Potential
	Transmembrane	339 – 359	21	Potential
	Topological domain	360 – 394	35	Extracellular Potential
	Transmembrane	395 – 415	21	Potential
	Topological domain	416 – 497	82	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9C0V1-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: F3A5212635AFB9C7	FASTA	497	53,387
	10 20 30 40 50 60 MSSTTDATPT PSGVNGGDSM TVNLNQFYNN GDVAWILTST ALVFIMIPGV GFFYSGLARR 70 80 90 100 110 120 RSAISMLFLS MMSVAIVAFQ WFFWGYSLTF SHEGGPYIGS LANFGLRQTL GRPSSGASSV 130 140 150 160 170 180 PDILFCVFQG MFAAITPALA IGAAADRGRM FPCMVFMFLW TSIVYDPIAF WTWNPNGWLN 190 200 210 220 230 240 KLGSYDFAGG SPVHISSGMA ALAYSIVIGK RCDHGTTKYR PHNVPHVVLG TVFLWFGWFG 250 260 270 280 290 300 FNGGSSAAAN MRGVMAVVVT HLAASVGGIV WCVIDFAKNR HWSVVGFCEG AVAGLVAITP 310 320 330 340 350 360 GSGFVPPWAA VVIGALGAVF CYAATYLKKI IRVDDALDIF AEHGVGGMVG NILTALFAAD 370 380 390 400 410 420 YIEALDGSGT AYTGGWITHH YIQLGYQLAD TVSCAAYSFA VSCALLFVMN YIPGLSLRVS 430 440 450 460 470 480 REDEVLGLDK IELGESAYYY KDSTDEPPPI TTSGVQYTSP TVSDSASNEK EQEHRAQNEA 490 QKEEEYRAES EAQAPAI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[2]	"Ammonium transporter genes in the fission yeast Schizosaccharomyces pombe: role in ammonium uptake and a morphological transition." Mitsuzawa H. Genes Cells 11:1183-1195(2006) [PubMed: 16999738] [Abstract] Cited for: FUNCTION. Strain: FY7406.
[3]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329672 Genomic DNA. Translation: CAC36934.1.
RefSeq	NP_588424.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2539332.
KEGG	spo:SPCPB1C11.01.
NMPDR	fig|4896.1.peg.762.
Organism-specific databases
GeneDB_Spombe	SPCPB1C11.01.
Gene expression databases
ArrayExpress	Q9C0V1.
Family and domain databases
InterPro	IPR001905. Ammonium_transpt. IPR010256. RH_like_transpt. [Graphical view]
PANTHER	PTHR11730. Ammonium_transpt. 1 hit.
Pfam	PF00909. Ammonium_transp. 1 hit. [Graphical view]
TIGRFAMs	TIGR00836. amt. 1 hit.
PROSITE	PS01219. AMMONIUM_TRANSP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMT1_SCHPO

Accession

Primary (citable) accession number: Q9C0V1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	June 1, 2001
Last modified:	November 25, 2008
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)