Reviewed, UniProtKB/Swiss-Prot Q9CA71 (FUT3_ARATH)

Names and origin

Protein names

Recommended name:
    Fucosyltransferase 3
      Short name=AtFUT3
    EC=2.4.1.-

Gene names

Name:	FUT3
Ordered Locus Names:	At1g74420
ORF Names:	F1M20.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	521 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May be involved in cell wall biosynthesis. May act as a fucosyltransferase.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane proteinBy similarity. Note= Membrane-bound form in trans cisternae of Golgi By similarity.
Tissue specificity	Expressed in roots, stems, leaves, flowers, siliques and seedlings.
Sequence similarities	Belongs to the glycosyltransferase 37 family.
Sequence caution	The sequence AAG52352.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall biogenesis Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	galactoside 2-alpha-L-fucosyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Notes: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9CA71-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 521	521	Fucosyltransferase 3		PRO_0000193912
Regions
	Topological domain	1 – 39	39	Cytoplasmic Potential
	Transmembrane	40 – 60	21	Signal-anchor for type II membrane protein Potential
	Topological domain	61 – 521	461	Lumenal Potential
	Compositional bias	404 – 407	4	Poly-Ser
Amino acid modifications
	Glycosylation	152	1	N-linked (GlcNAc...) Potential
	Glycosylation	222	1	N-linked (GlcNAc...) Potential
	Glycosylation	493	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 29, 2007. Version 3. Checksum: D2AB9A0FD84CAFFC	FASTA	521	58,989
	10 20 30 40 50 60 MKRGKKNSDA GDRLTNSDTR TGSSELNAMM KPSLSSMKTM GLLLAVLMVA SVMFSLSVVL 70 80 90 100 110 120 RDPPSDDVIE TEAASRVLQS RLHQDGGLSE KKAQLGNINL VPSFDKESCL SRYEASLYRK 130 140 150 160 170 180 ESPFKQSSYL DYRLQRYEDL HRRCGPFTRS YNLTLDKLKS GDRSDGEVSG CRYVIWLNSN 190 200 210 220 230 240 GDLGNRMLSL ASAFLYALLT NRFLLVELGV DMADLFCEPF PNTTWFLPPE FPLNSHFNEQ 250 260 270 280 290 300 SLLRNSGNPM VAYRHVVRDS SDQQKLFFCE DSQVLLEETP WLILKADSFF LPSLFSVSSF 310 320 330 340 350 360 KQELQMLFPE KDTAFHFLSQ YLFHPTNVVW GLITRYYNAY LAKADQRIGI YIGVSESGNE 370 380 390 400 410 420 QFQHLIDQIL ACGTRHKLLP EVDKQRNLPS SQVLNRKSKA VFISSSSPGY FKSIRDVYWE 430 440 450 460 470 480 NPTVMGEIIS VHKPSYKDYQ KTPRNMESKR AWAEIYLLSC SDALVVTGLW SSLVEVAHGL 490 500 510 520 GGLKPWVLNK AENGTAHEPY CVKARSIEPC SQATLFHGCK D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Characterization of a family of Arabidopsis genes related to xyloglucan fucosyltransferase1." Sarria R., Wagner T.A., O'Neill M.A., Faik A., Wilkerson C.G., Keegstra K., Raikhel N.V. Plant Physiol. 127:1595-1606(2001) [PubMed: 11743104] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-521.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC011765 Genomic DNA. Translation: AAG52352.1. Sequence problems. AK175419 mRNA. Translation: BAD43182.1. AK176710 mRNA. Translation: BAD44473.1. AF417473 mRNA. Translation: AAL50622.1. Different initiation.
RefSeq	NP_177582.3.
UniGene	At.28630
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	843783.
GenomeReviews	Gene locus AT1G74420 in contig CT485782_GR.
KEGG	ath:AT1G74420.
NMPDR	fig|3702.1.peg.6954.
Organism-specific databases
TAIR	At1g74420.
Family and domain databases
InterPro	IPR004938. XG_FTase. [Graphical view]
Pfam	PF03254. XG_FTase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUT3_ARATH

Accession

Primary (citable) accession number: Q9CA71
Secondary accession number(s): Q67XV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	May 29, 2007
Last modified:	November 25, 2008
This is version 43 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)