Reviewed, UniProtKB/Swiss-Prot Q9DCU9 (DAPAL_MOUSE)

Names and origin

Protein names	Recommended name:     Dihydrodipicolinate synthase-like, mitochondrial       Short name=DHDPS-like protein     EC=4.-.-.-
Organism	Mus musculus (Mouse)
Taxonomic identifier	10090 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probable lyase By similarity.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the DHDPS family.
Sequence caution	The sequence AAH16430.1 differs from that shown. Reason: Erroneous termination at position 322. Translated as stop. The sequence BAB27226.1 differs from that shown. Reason: Frameshift at position 270.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Schiff base
Molecular function	Lyase
Gene Ontology (GO)
Cellular component	mitochondrion Ref.3 Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Transit peptide	1 – 25	25	Mitochondrion Potential
	Chain	26 – 321	296	Dihydrodipicolinate synthase-like, mitochondrial
Regions
	Region	71 – 72	2	Substrate binding By similarity
Sites
	Active site	190	1	Schiff-base intermediate with substrate By similarity
	Site	162	1	Involved in proton transfer during cleavage By similarity
Experimental info
	Sequence conflict	25	1	K → M in BAB27226. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9DCU9-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 75B3DC959756D5A7	FASTA	321	34,644
	10 20 30 40 50 60 MLGPQIWASM RQGLSRGLSR NVKGKKVDIA GIYPPVTTPF TATAEVDYGK LEENLNRLAT 70 80 90 100 110 120 FPFRGFVVQG STGEFPFLTS LERLEVVSRV RQAIPKDKFL IAGSGCESTQ ATVEMTVSMA 130 140 150 160 170 180 QVGADVAMVV TPCYYRGRMS SAALIHHYTK VADVSPIPVV LYSVPANTGL ELPVDAVVTL 190 200 210 220 230 240 SQHPNIIGLK DSGGDVTRIG LIVHKTSKQD FQVLAGSAGF LLASYAVGAV GGICGLANVL 250 260 270 280 290 300 GAQVCQLERL CLTGQWEAAQ ELQHRLIEPN TAVTRRFGIP GLKKTMDWFG YYGGPCRAPL 310 320 QELSPTEEEA LRLDFSNNGW L

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Kidney and Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Kidney.
[3]	"Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria." Mootha V.K., Bunkenborg J., Olsen J.V., Hjerrild M., Wisniewski J.R., Stahl E., Bolouri M.S., Ray H.N., Sihag S., Kamal M., Patterson N., Lander E.S., Mann M. Cell 115:629-640(2003) [PubMed: 14651853] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK002457 mRNA. Translation: BAB22114.1. AK010857 mRNA. Translation: BAB27226.1. Frameshift. BC016430 mRNA. Translation: AAH16430.1. Sequence problems.
RefSeq	NP_080428.1.
UniGene	Mm.24196
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000025176. Mus musculus. [Contig view]
GeneID	67432.
KEGG	mmu:67432.
Organism-specific databases
MGI	MGI:1914682. 0610010D20Rik.
Phylogenomic databases
HOVERGEN	Q9DCU9.
Gene expression databases
ArrayExpress	Q9DCU9.
CleanEx	MM_0610010D20RIK.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR002220. DHDPS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PRINTS	PR00146. DHPICSNTHASE.
ProDom	PD001859. DHDPS. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00665. DHDPS_1. False negative. PS00666. DHDPS_2. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

DAPAL_MOUSE

Accession

Primary (citable) accession number: Q9DCU9
Secondary accession number(s): Q91W74, Q9CY60

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	June 1, 2001
Last modified:	July 22, 2008
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)