Reviewed, UniProtKB/Swiss-Prot Q9ERZ4 (ACM2_MOUSE)

Names and origin

Protein names

Recommended name:
    Muscarinic acetylcholine receptor M2

Gene names

Name:	Chrm2
Synonyms:	Chrm-2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	acetylcholine receptor signaling, muscarinic pathway Inferred from mutant phenotype. Source: MGI
Molecular function	muscarinic acetylcholine receptor activity Inferred from mutant phenotype. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 466	466	Muscarinic acetylcholine receptor M2
Regions
	Topological domain	1 – 22	22	Extracellular Potential
	Transmembrane	23 – 45	23	1 Potential
	Topological domain	46 – 59	14	Cytoplasmic Potential
	Transmembrane	60 – 80	21	2 Potential
	Topological domain	81 – 97	17	Extracellular Potential
	Transmembrane	98 – 119	22	3 Potential
	Topological domain	120 – 139	20	Cytoplasmic Potential
	Transmembrane	140 – 162	23	4 Potential
	Topological domain	163 – 184	22	Extracellular Potential
	Transmembrane	185 – 207	23	5 Potential
	Topological domain	208 – 388	181	Cytoplasmic Potential
	Transmembrane	389 – 409	21	6 Potential
	Topological domain	410 – 423	14	Extracellular Potential
	Transmembrane	424 – 443	20	7 Potential
	Topological domain	444 – 466	23	Cytoplasmic Potential
Amino acid modifications
	Modified residue	232	1	Phosphoserine
	Modified residue	234	1	Phosphoserine
	Modified residue	446	1	Phosphothreonine Potential
	Modified residue	450	1	Phosphothreonine Potential
	Modified residue	465	1	Phosphothreonine Potential
	Glycosylation	2	1	N-linked (GlcNAc...) Potential
	Glycosylation	3	1	N-linked (GlcNAc...) Potential
	Glycosylation	6	1	N-linked (GlcNAc...) Potential
	Disulfide bond	96 ↔ 176		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ERZ4-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: CDCF281AD28276F8	FASTA	466	51,515
	10 20 30 40 50 60 MNNSTNSSNN GLAITSPYKT FEVVFIVLVA GSLSLVTIIG NILVMVSIKV NRHLQTVNNY 70 80 90 100 110 120 FLFSLACADL IIGVFSMNLY TLYTVIGYWP LGPVVCDLWL ALDYVVSNAS VMNLLIISFD 130 140 150 160 170 180 RYFCVTKPLT YPVKRTTKMA GMMIAAAWVL SFILWAPAIL FWQFIVGVRT VEDGECYIQF 190 200 210 220 230 240 FSNAAVTFGT AIAAFYLPVI IMTVLYWHIS RASKSRIKKE KKEPVANQDP VSPSLVQGRI 250 260 270 280 290 300 VKPNNNNMPG GDGGLEHNKI QNGKALRDGG TENCVQGEEK ESSNDSTSVS AVASNMRDDE 310 320 330 340 350 360 ITQDENTVST SLGHSKDDNS RQTCIKIVTK TQKGDACTPT STTVELVGSS GQNGDEKQNI 370 380 390 400 410 420 VARKIVKMTK QPAKKKPPPS REKKVTRTIL AILLAFIITW APYNVMVLIN TFCAPCIPNT 430 440 450 460 VWTIGYWLCY INSTINPACY ALCNATFKKT FKHLLMCHYK NIGATR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation, sequence and functional expression of mouse muscarinic acetylcholine receptor genes." Gomeza J., Wess J. Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed: 17114649] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232 AND SER-234, MASS SPECTROMETRY. Tissue: Brain cortex.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF264049 Genomic DNA. Translation: AAG14343.1.
RefSeq	NP_987076.1.
UniGene	Mm.448632
3D structure databases
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q9ERZ4.
Genome annotation databases
Ensembl	ENSMUSG00000045613. Mus musculus. [Contig view]
GeneID	243764.
KEGG	mmu:243764.
Organism-specific databases
MGI	MGI:88397. Chrm2.
Phylogenomic databases
HOGENOM	Q9ERZ4.
HOVERGEN	Q9ERZ4.
Gene expression databases
CleanEx	MM_CHRM2.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR001065. Musac_M2_rcpt. IPR000995. Musac_rcpt. [Graphical view]
PANTHER	PTHR19266:SF32. Musac_M2_rcpt. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00243. MUSCARINICR. PR00539. MUSCRINICM2R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProDom	Q9ERZ4. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

ACM2_MOUSE

Accession

Primary (citable) accession number: Q9ERZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	March 1, 2001
Last modified:	July 22, 2008
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)