Reviewed, UniProtKB/Swiss-Prot Q9FJP9 (DHSB3_ARATH)

Names and origin

Protein names

Recommended name:
    Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 3, mitochondrial
    EC=1.3.5.1
Alternative name(s):
    Iron-sulfur subunit of complex II
      Short name=Ip

Gene names

Name:	SDH2-3
Ordered Locus Names:	At5g65165
ORF Names:	MQN23.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) By similarity.
Catalytic activity	Succinate + ubiquinone = fumarate + ubiquinol.
Cofactor	Binds 1 2Fe-2S cluster By similarity. Binds 1 3Fe-4S cluster By similarity. Binds 1 4Fe-4S cluster By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b composed of a large and a small subunit By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix sideBy similarity.
Developmental stage	Expressed during seed development. Detected during seed maturation and decreased during germination.
Sequence similarities	Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 4Fe-4S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	2Fe-2S 3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Transit peptide	1 – 22	22	Mitochondrion Potential
	Chain	23 – 309	287	Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 3, mitochondrial
Regions
	Domain	69 – 160	92	2Fe-2S ferredoxin-type
	Domain	202 – 232	31	4Fe-4S ferredoxin-type
Sites
	Metal binding	120	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	125	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	128	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	140	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	212	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	215	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	218	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	222	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	270	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	276	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	280	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Binding site	227	1	Ubiquinone; shared with DHSD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9FJP9-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: F69189C234D87A1D	FASTA	309	34,983
	10 20 30 40 50 60 MSSVLRLLGR RICNPAAEKV RLSSSLSGGG DFPILNGHKA AQDLSKDTLK SQDITKEKEG 70 80 90 100 110 120 QHKEVKKEFK IYRWNPDKPN SKPFLQSFFV DLSSCGPMVL DVLQKIKAED DASLSYRRSC 130 140 150 160 170 180 REGICGSCSM NIDGTNTVAC LKPINPNTSK PTIITPLPHM YVIKDLVVDL TNFYQQYKSM 190 200 210 220 230 240 EPWLKTRKPP KDGREHRQSP KDRKKLDGLY ECILCACCTT SCPSYWWNPE EFPGPAALLQ 250 260 270 280 290 300 AYRWISDSRD EYREERLQAI TESETKVYRC RAIKNCTATC PKGLNPASAI LKMKSKHLLS DPLVRTESV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Three different genes encode the iron-sulphur subunit of succinate dehydrogenase in Arabidopsis thaliana." Figueroa P., Leon G., Elorza A., Holuigue L., Jordana X. Plant Mol. Biol. 46:241-250(2001) [PubMed: 11442063] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Leaf.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones." Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S. DNA Res. 5:203-216(1998) [PubMed: 9734815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"A nuclear gene for the iron-sulfur subunit of mitochondrial complex II is specifically expressed during Arabidopsis seed development and germination." Elorza A., Roschzttardtz H., Gomez I., Mouras A., Holuigue L., Araya A., Jordana X. Plant Cell Physiol. 47:14-21(2006) [PubMed: 16249327] [Abstract] Cited for: DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ278912 mRNA. Translation: CAC19857.1. AB013395 Genomic DNA. Translation: BAB11652.1.
RefSeq	NP_680465.2.
UniGene	At.45474
3D structure databases
HSSP	HSSP built from PDB template 1NEK based on UniProtKB P07014.
ModBase	Search...
Genome annotation databases
GeneID	836640.
GenomeReviews	Gene locus AT5G65165 in contig BA000015_GR.
KEGG	ath:AT5G65165.
NMPDR	fig|3702.1.peg.28594.
Organism-specific databases
GeneFarm	2172. 175.
TAIR	At5g65165.
Family and domain databases
InterPro	IPR006058. 2Fe2S_fd_BS. IPR001450. 4Fe4S_Fe_S_bd. IPR012675. b-grasp_ferredoxin-like. IPR001041. Ferredoxin. IPR012285. Fum_reductase_C. IPR004489. Succ_DHase/fum_Rdtase_Fe-S. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit. G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
TIGRFAMs	TIGR00384. dhsB. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. False negative. PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 1 hit. [Graphical view]
ProDom	Q9FJP9. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

DHSB3_ARATH

Accession

Primary (citable) accession number: Q9FJP9
Secondary accession number(s): Q9G3L8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	March 1, 2001
Last modified:	September 2, 2008
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)