Reviewed,
UniProtKB/Swiss-Prot Q9JK82 (EXT1_CRIGR)
Last modified
November 25, 2008.
Version 43.
History...
Clusters with 100%,
90%,
50% identity |
 Documents (2) |
 Third-party data |
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Names and origin
| Protein names | Recommended name: Exostosin-1 EC=2.4.1.224 EC=2.4.1.225 Alternative name(s): Heparan sulfate copolymerase Multiple exostoses protein 1 homolog | ||
| Gene names |
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| Organism | Cricetulus griseus (Chinese hamster) | ||
| Taxonomic identifier | 10029 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus |
Protein attributes
| Sequence length | 746 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Glycosyltransferase required for the biosynthesis of heparan-sulfate and responsible for the alternating addition of beta-1-4-linked glucuronic acid (GlcA) and alpha-1-4-linked N-acetylglucosamine (GlcNAc) units to nascent heparan sulfate chains. |
| Catalytic activity | UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan = UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan. UDP-alpha-D-glucuronate + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan. |
| Cofactor | Divalent cations. |
| Pathway | |
| Subunit structure | Forms a homo/hetero-oligomeric complex with EXT2 By similarity. |
| Subcellular location | Endoplasmic reticulum membrane; Single-pass type II membrane proteinBy similarity. Golgi apparatus membrane; Single-pass type II membrane proteinBy similarity. Note= The EXT1/EXT2 complex is localized in the Golgi apparatus By similarity. |
| Sequence similarities | Belongs to the glycosyltransferase 47 family. |
Ontologies
Keywords | |
|---|---|
| Cellular component | Endoplasmic reticulum Golgi apparatus Membrane |
| Domain | Signal-anchor Transmembrane |
| Molecular function | Glycosyltransferase Transferase |
| PTM | Glycoprotein |
Gene Ontology (GO) | |
| Biological process | heparan sulfate proteoglycan biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB |
| Cellular component | Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to endoplasmic reticulum membraneInferred from sequence or structural similarity. Source: UniProtKB |
| Molecular function | N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activityInferred from sequence or structural similarity. Source: UniProtKB |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 746 | 746 | Exostosin-1 | PRO_0000149647 | |||||
Regions | |||||||||
| Topological domain | 1 – 5 | 5 | Cytoplasmic Potential | ||||||
| Transmembrane | 6 – 26 | 21 | Signal-anchor for type II membrane protein Potential | ||||||
| Topological domain | 27 – 746 | 720 | Lumenal Potential | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 89 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 330 | 1 | N-linked (GlcNAc...) Potential | ||||||
Experimental info | |||||||||
| Mutagenesis | 268 | 1 | G → E: Reduced GlcA transferase activity | ||||||
| Mutagenesis | 298 | 1 | C → Y: Reduced GlcA transferase activity | ||||||
| Mutagenesis | 341 | 1 | R → K: Reduced GlcA transferase activity | ||||||
| Mutagenesis | 344 | 1 | S → F: Reduced GlcA transferase activity | ||||||
| Mutagenesis | 346 | 1 | R → K: Reduced GlcA transferase activity | ||||||
| Mutagenesis | 349 | 1 | E → K: Reduced GlcA transferase activity | ||||||
Sequences
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References
| [1] | "Location of the glucuronosyltransferase domain in the heparan sulfate copolymerase EXT1 by analysis of Chinese hamster ovary cell mutants." Wei G., Bai X., Gabb M.M.G., Bame K.J., Koshy T.I., Spear P.G., Esko J.D. J. Biol. Chem. 275:27733-27740(2000) [PubMed: 10864928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION OF MUTANTS GLY-268; CYS-298; ARG-341; SER-344; ARG-346 AND GLU-349. |
Cross-references
Sequence databases | |
|---|---|
| AF252858 mRNA. Translation: AAF71276.1. | |
3D structure databases | |
| HSSP | HSSP built from PDB template 1OMX based on UniProtKB Q9ES89. |
| ModBase | Search... |
Phylogenomic databases | |
| HOVERGEN | Q9JK82. |
Family and domain databases | |
| InterPro | IPR004263. Exostosin. IPR015338. HexNAc_Trfase_a. [Graphical view] |
| Pfam | PF03016. Exostosin. 1 hit. PF09258. EXTL2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | EXT1_CRIGR | ||||||||
| Accession | Primary (citable) accession number: Q9JK82 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
