Reviewed, UniProtKB/Swiss-Prot Q9KDE6 (SYA_BACHD)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	BH1267

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	879 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 879	879	Alanyl-tRNA synthetase

Sequences

	Sequence		Length	Mass (Da)
	Q9KDE6-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 4BC9C5CC397A7F41	FASTA	879	97,742
	10 20 30 40 50 60 MKYLTSAQVR QMFLDFFKEK GHDVEPSASL VPHDDPSLLW INSGVATLKK YFDGRVIPEN 70 80 90 100 110 120 PRITNAQKSI RTNDIENVGK TARHHTFFEM LGNFSIGDYF KEEAIEWAWE FLTSEKWIGF 130 140 150 160 170 180 DKEKLSVTVH PEDDEAYSYW KEKIGIPEER IIRLEGNFWD IGEGPSGPNT EIFYDRGPEY 190 200 210 220 230 240 GDQPNDPELY PGGENDRYLE VWNLVFSQFN HNPDGSYTPL PKKNIDTGMG LERMVSVIQN 250 260 270 280 290 300 VPTNFETDLF MPIIRATEKI SGTEYGSHHE ADVSFKVIAD HIRTVTFAIG DGALPSNEGR 310 320 330 340 350 360 GYVLRRLLRR AVRYAKQIGI DRPFMYELVP VVGDIMVDFY PEVKEKAAFI QKVVKTEEER 370 380 390 400 410 420 FHETLNEGLS ILEKVIDKAK SEGASTISGS DVFRLYDTYG FPVDLTEEYV EEQGLQVDLD 430 440 450 460 470 480 GFEAEMERQR ERARTARQQA GSMQVQDEVL GQITVDSTFI GYKQLSTETT IETIVLDKTV 490 500 510 520 530 540 ADYVGAGQEA KVILKETPFY AESGGQVADK GIIRGANGFA VVSDVQKAPN GQHLHTVIVK 550 560 570 580 590 600 EGTLQVNDQV QAIVEETERS GIVKNHTATH LLHRALKDVL GEHVNQAGSL VSEERLRFDF 610 620 630 640 650 660 SHFGQVTDEE KEKIERIVNE KIWQAIKVNI STKTLDEAKA IGAMALFGEK YGDIVRVVEV 670 680 690 700 710 720 GDYSIELCGG CHVTNTSEIG LFKIVSESGI GAGVRRIEAV TGKEAFLFMA KQLDLLKETA 730 740 750 760 770 780 ATVKAKNVKD VPVRVEALQQ QIRELQRENE SLNAKLGNME AGSLVNEVQK IEGVPVLAKA 790 800 810 820 830 840 ISGADMDGLR SIVDKLKQEI PSVVIVLGTA SEGKVNIVAG VTKDLINKGY HAGKLVKEVA 850 860 870 TRCGGGGGGR PDMAQAGGKQ PEKLQDALSF VYEYVKSIS

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000004 Genomic DNA. Translation: BAB04986.1.
PIR	C83808.
RefSeq	NP_242133.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	894263.
GenomeReviews	Gene locus BH1267 in contig BA000004_GR.
KEGG	bha:BH1267.
NMPDR	fig|272558.1.peg.1267.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9KDE6.
Enzyme and pathway databases
BioCyc	BHAL272558:BH1267-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	Q9KDE6. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_BACHD

Accession

Primary (citable) accession number: Q9KDE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	October 1, 2000
Last modified:	July 22, 2008
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)