Reviewed, UniProtKB/Swiss-Prot Q9LWR0 (TIP42_ORYSJ)

Names and origin

Protein names

Recommended name:
    Probable aquaporin TIP4-2
Alternative name(s):
    Tonoplast intrinsic protein 4-2
      Short name=OsTIP4;2

Gene names

Name:	TIP4-2
Ordered Locus Names:	Os01g0232100, LOC_Os01g13130
ORF Names:	P0431F01.26

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Aquaporins facilitate the transport of water and small neutral solutes across cell membranes. May be involved in transport from the vacuolar compartment to the cytoplasm By similarity.
Subcellular location	Vacuole membrane; Multi-pass membrane proteinBy similarity. Note= Tonoplast.
Tissue specificity	Expressed in roots, leaves and anthers.
Domain	Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Sequence similarities	Belongs to the MIP/aquaporin (TC 1.A.8) family. TIP subfamily. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane Vacuole
Domain	Repeat Transmembrane
Gene Ontology (GO)
Cellular component	vacuolar membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 256	256	Probable aquaporin TIP4-2
Regions
	Transmembrane	25 – 45	21	1 Potential
	Transmembrane	59 – 79	21	2 Potential
	Transmembrane	86 – 108	23	3 Potential
	Transmembrane	146 – 166	21	4 Potential
	Transmembrane	178 – 198	21	5 Potential
	Transmembrane	220 – 240	21	6 Potential
	Motif	87 – 89	3	NPA 1
	Motif	201 – 203	3	NPA 2

Sequences

	Sequence		Length	Mass (Da)
	Q9LWR0-1 [UniParc]. Last modified May 1, 2007. Version 2. Checksum: F7AB9405FD24B8BC	FASTA	256	25,599
	10 20 30 40 50 60 MPLLPMTKLE LGHRGEAWEP GCLRAVAGEL LFTFLFVFIG VASTITAGKA AGGAGEAAAV 70 80 90 100 110 120 TAAAMAQALV VAVLATAGFH VSGGHLNPAV TLSLAVGGHI TLFRSALYVA AQLAGSSLAC 130 140 150 160 170 180 LLLRCLTGGA ATPVHALADG VGPVQGVAAE AVFTFTLLLV ICATILDPRR AAPPGTGPLL 190 200 210 220 230 240 TGLLVGANTV AGGALTGASM NPARSFGPAL ATGEWAHHWV YWVGPLAGGP LAVVAYELLF 250 MDVEDAGGAH QPLPQE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[5]	"Identification of 33 rice aquaporin genes and analysis of their expression and function." Sakurai J., Ishikawa F., Yamaguchi T., Uemura M., Maeshima M. Plant Cell Physiol. 46:1568-1577(2005) [PubMed: 16033806] [Abstract] Cited for: NOMENCLATURE, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP001550 Genomic DNA. Translation: BAA92993.1. Different initiation. AP008207 Genomic DNA. Translation: BAF04415.1. AK099190 mRNA. No translation available.
RefSeq	NP_001042501.1.
UniGene	Os.52849
3D structure databases
HSSP	HSSP built from PDB template 1H6I based on UniProtKB P29972.
ModBase	Search...
Genome annotation databases
GeneID	4324277.
KEGG	osa:4324277.
NMPDR	fig|39947.1.peg.5582.
Organism-specific databases
Gramene	Q9LWR0.
Family and domain databases
InterPro	IPR000425. MIP. [Graphical view]
Gene3D	G3DSA:1.20.1080.10. MIP. 1 hit.
PANTHER	PTHR19139. MIP. 1 hit.
Pfam	PF00230. MIP. 1 hit. [Graphical view]
PRINTS	PR00783. MINTRINSICP.
ProDom	PD000295. MIP. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00221. MIP. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

TIP42_ORYSJ

Accession

Primary (citable) accession number: Q9LWR0
Secondary accession number(s): Q0JPB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	May 1, 2007
Last modified:	July 22, 2008
This is version 50 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)