Reviewed, UniProtKB/Swiss-Prot Q9LWR2 (TIP43_ORYSJ)

Names and origin

Protein names

Recommended name:
    Probable aquaporin TIP4-3
Alternative name(s):
    Tonoplast intrinsic protein 4-3
      Short name=OsTIP4;3

Gene names

Name:	TIP4-3
Ordered Locus Names:	Os01g0232000, LOC_Os01g13120
ORF Names:	P0431F01.24

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Aquaporins facilitate the transport of water and small neutral solutes across cell membranes. May be involved in transport from the vacuolar compartment to the cytoplasm By similarity.
Subcellular location	Vacuole membrane; Multi-pass membrane proteinBy similarity. Note= Tonoplast.
Tissue specificity	Expressed in leaves.
Domain	Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Sequence similarities	Belongs to the MIP/aquaporin (TC 1.A.8) family. TIP subfamily. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane Vacuole
Domain	Repeat Transmembrane
Gene Ontology (GO)
Cellular component	vacuolar membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 251	251	Probable aquaporin TIP4-3
Regions
	Transmembrane	20 – 40	21	1 Potential
	Transmembrane	52 – 72	21	2 Potential
	Transmembrane	101 – 121	21	3 Potential
	Transmembrane	142 – 162	21	4 Potential
	Transmembrane	167 – 187	21	5 Potential
	Transmembrane	215 – 235	21	6 Potential
	Motif	82 – 84	3	NPA 1
	Motif	196 – 198	3	NPA 2

Sequences

	Sequence		Length	Mass (Da)
	Q9LWR2-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: E1883CB8F62FE9B0	FASTA	251	25,053
	10 20 30 40 50 60 MAKLALGHHR EATDPGCLRA VVAELLLTFL FVFSGVGSAM AAAKLGGGGD TIMGLTAVAA 70 80 90 100 110 120 AHALVVAVMV SAGLHVSGGH INPAVTLGLA AGGHITLFRS ALYAAAQLLG SSLACLLLAA 130 140 150 160 170 180 LTGGEEAVPV HAPAPGVGAA RAVAMEAVLT FSLLFAVYAT VVDRRRAVGA LGPLLVGLVV 190 200 210 220 230 240 GANILAGGPY SGASMNPARS FGPALAAGEW ADHWIYWVGP LIGGPLAGLV YEGLFMGPPG 250 HEPLPRNDGD F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 106-251. Strain: cv. Nipponbare.
[5]	"Identification of 33 rice aquaporin genes and analysis of their expression and function." Sakurai J., Ishikawa F., Yamaguchi T., Uemura M., Maeshima M. Plant Cell Physiol. 46:1568-1577(2005) [PubMed: 16033806] [Abstract] Cited for: NOMENCLATURE, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP001550 Genomic DNA. Translation: BAA92991.1. AP008207 Genomic DNA. Translation: BAF04414.1. AK069192 mRNA. No translation available.
RefSeq	NP_001042500.1.
UniGene	Os.20516
3D structure databases
HSSP	HSSP built from PDB template 1H6I based on UniProtKB P29972.
ModBase	Search...
Genome annotation databases
GeneID	4324276.
KEGG	osa:4324276.
NMPDR	fig|39947.1.peg.5580.
Organism-specific databases
Gramene	Q9LWR2.
Family and domain databases
InterPro	IPR012269. Aquaporin. IPR000425. MIP. [Graphical view]
Gene3D	G3DSA:1.20.1080.10. MIP. 1 hit.
PANTHER	PTHR19139. MIP. 1 hit.
Pfam	PF00230. MIP. 1 hit. [Graphical view]
PRINTS	PR00783. MINTRINSICP.
ProDom	PD000295. MIP. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00861. MIP. 1 hit.
PROSITE	PS00221. MIP. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

TIP43_ORYSJ

Accession

Primary (citable) accession number: Q9LWR2
Secondary accession number(s): Q0JPB4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	October 1, 2000
Last modified:	July 22, 2008
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)