Reviewed, UniProtKB/Swiss-Prot Q9N2D2 (CHYM_CALJA)

Names and origin

Protein names

Recommended name:
    Chymosin
    EC=3.4.23.4
Alternative name(s):
    Preprorennin

Gene names

Name:

CYM

Organism

Callithrix jacchus (Common marmoset)

Taxonomic identifier

9483 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Platyrrhini › Cebidae › Callitrichinae › Callithrix

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydrolyzes a variety of proteins.
Catalytic activity	Broad specificity similar to that of pepsin A. Clots milk by cleavage of a single 105-Ser-Phe-|-Met-Ala-108 bond in kappa-chain of casein.
Enzyme regulation	Inhibited by pepstatin.
Subunit structure	Monomer By similarity.
Developmental stage	Expressed in adult, not neonate-specific as in other organisms.
Sequence similarities	Belongs to the peptidase A1 family.
Biophysicochemical properties	pH dependence: Optimum pH is about 2.5.

Ontologies

Keywords
Biological process	Digestion
Domain	Repeat Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	chymosin activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 16	16
	Propeptide	17 – 58	42	Activation peptide By similarity
	Chain	59 – 381	323	Chymosin
Regions
	Repeat	92 – 102	11	1
	Repeat	274 – 284	11	2
Sites
	Active site	92	1	By similarity
	Active site	274	1	By similarity
Amino acid modifications
	Disulfide bond	105 ↔ 110		By similarity
	Disulfide bond	265 ↔ 269		By similarity
	Disulfide bond	308 ↔ 341		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9N2D2-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: C5820C74C97BB96B	FASTA	381	41,896
	10 20 30 40 50 60 MRGFVVLLAV FALSQASGIV RIPLHKGKSL RRALKERGLL EDFLKNHQHA VSRKHSNSRE 70 80 90 100 110 120 VASEFLTNYL DCQYFGKIYI GTPPQEFTVV FDTGSSDLWV PSVYCNSVAC QNHHRFDPSK 130 140 150 160 170 180 SSTFQNMDKS LSIQYGTGSM QGLLGYDTVT VSSIVDPHQT VGLSTQEPGD VFTYSEFDGI 190 200 210 220 230 240 LGLAYPSLAS EYSVPVFDNM MDRHLVAQDL FSVYMSRNEQ GSMLTLGAID PSYYTGSLHW 250 260 270 280 290 300 IPVTVQEYWQ FTVDSVTVDG VVVACDGGCQ AILDTGTSML VGPGSDIFNI QQAIGATEGQ 310 320 330 340 350 360 YGEFDIDCGT LSSMPTVVFE INGKKYPLPP SAYTNQDQGF CTSGFQGDDS SQQWILGDVF 370 380 IREYYSVFDR ASNLVGLAKA I

References

[1]

"New World monkey pepsinogens A and C, and prochymosins. Purification, characterization of enzymatic properties, cDNA cloning, and molecular evolution." Kageyama T. J. Biochem. 127:761-770(2000) [PubMed: 10788784] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 17-26, FUNCTION, ENZYME REGULATION. Tissue: Gastric mucosa.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB038386 mRNA. Translation: BAA90873.1.
PIR	JC7247.
3D structure databases
HSSP	HSSP built from PDB template 4CMS based on UniProtKB P00794.
SMR	Q9N2D2. Positions 60-381.
ModBase	Search...
Protein family/group databases
MEROPS	A01.006.
Phylogenomic databases
HOVERGEN	Q9N2D2.
Family and domain databases
InterPro	IPR001969. Pept_Asp_AS. IPR009007. Pept_Aspartc_cat. IPR001461. Peptidase_A1. IPR012848. Propep_A1. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 1 hit.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF07966. A1_Propeptide. 1 hit. PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
ProDom	Q9N2D2. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	Q9N2D2.
ProtoNet	Search...

Entry information

Entry name

CHYM_CALJA

Accession

Primary (citable) accession number: Q9N2D2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	October 1, 2000
Last modified:	July 22, 2008
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)