Rho guanine nucleotide exchange factor 3

Names and origin

Protein names

Recommended name:
    Rho guanine nucleotide exchange factor 3
Alternative name(s):
    Exchange factor found in platelets and leukemic and neuronal tissues
      Short name=XPLN

Gene names

Name:

ARHGEF3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	Acts as guanine nucleotide exchange factor (GEF) for RhoA and RhoB GTPases.
Subunit structure	Interacts with RHOA and RHOB.
Subcellular location	CytoplasmBy similarity.
Tissue specificity	Widely expressed. Highest levels are found in adult brain and skeletal muscle. Lower levels are found in heart and kidney.
Sequence similarities	Contains 1 DH (DBL-homology) domain. Contains 1 PH domain.

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Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	Guanine-nucleotide releasing factor
Gene Ontology (GO)
Biological process	Rho protein signal transduction Ref.1 Traceable author statement. Source: ProtInc
Cellular component	intracellular Inferred from direct assay. Source: LIFEdb
Molecular function	Rho guanyl-nucleotide exchange factor activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

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Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NR81-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NR81-2) The sequence of this isoform differs from the canonical sequence as follows: 1-32: MVAKDYPFYLTVKRANCSLELPPASGPAKDAE → MDSSTAMNQC...AVSLCSLDIS
Notes: No experimental confirmation available.

Isoform 3 (identifier: Q9NR81-3) The sequence of this isoform differs from the canonical sequence as follows: 1-32: MVAKDYPFYLTVKRANCSLELPPASGPAKDAE → MIEVCHHNWLLWLCPSKFGIFMCCLASTTGQMELRRTR
Notes: No experimental confirmation available.

Isoform 4 (identifier: Q9NR81-4) The sequence of this isoform differs from the canonical sequence as follows: 1-202: Missing. 203-204: GW → MK
Notes: No experimental confirmation available.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Chain

1 – 526

526

Rho guanine nucleotide exchange factor 3

Regions

Domain

122 – 304

183

DH

Domain

291 – 449

159

PH

Natural variations

Alternative sequence

1 – 202

202

Missing in isoform 4.

Alternative sequence

1 – 32

32

MVAKD…AKDAE → MDSSTAMNQCSCRGMEENKE RPKRQRQNNFPMFPSPKAWN FRGRKRKQSTQDEDAVSLCS LDIS in isoform 2.

Alternative sequence

1 – 32

32

MVAKD…AKDAE → MIEVCHHNWLLWLCPSKFGI FMCCLASTTGQMELRRTR in isoform 3.

Alternative sequence

203 – 204

2

GW → MK in isoform 4.

Natural variant

13

1

K → R: dbSNP rs3732507.

Natural variant

335

1

L → V: dbSNP rs3772219.

Experimental info

Sequence conflict

410

1

I → T in AAP97313. Ref.3

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Sequences

Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 0FE8248AB52D1CBC Show »	FASTA	526	59,783
10 20 30 40 50 60 MVAKDYPFYL TVKRANCSLE LPPASGPAKD AEEPSNKRVK PLSRVTSLAN LIPPVKATPL 70 80 90 100 110 120 KRFSQTLQRS ISFRSESRPD ILAPRPWSRN AAPSSTKRRD SKLWSETFDV CVNQMLTSKE 130 140 150 160 170 180 IKRQEAIFEL SQGEEDLIED LKLAKKAYHD PMLKLSIMTE QELNQIFGTL DSLIPLHEEL 190 200 210 220 230 240 LSQLRDVRKP DGSTEHVGPI LVGWLPCLSS YDSYCSNQVA AKALLDHKKQ DHRVQDFLQR 250 260 270 280 290 300 CLESPFSRKL DLWNFLDIPR SRLVKYPLLL REILRHTPND NPDQQHLEEA INIIQGIVAE 310 320 330 340 350 360 INTKTGESEC RYYKERLLYL EEGQKDSLID SSRVLCCHGE LKNNRGVKLH VFLFQEVLVI 370 380 390 400 410 420 TRAVTHNEQL CYQLYRQPIP VKDLLLEDLQ DGEVRLGGSL RGAFSNNERI KNFFRVSFKN 430 440 450 460 470 480 GSQSQTHSLQ ANDTFNKQQW LNCIRQAKET VLCAAGQAGV LDSEGSFLNP TTGSRELQGE 490 500 510 520 TKLEQMDQSD SESDCSMDTS EVSLDCERME QTDSSCGNSR HGESNV « Hide
Isoform 2 [UniParc]. Checksum: C01C434F0858A58C Show »		558	63,755
10 20 30 40 50 60 MDSSTAMNQC SCRGMEENKE RPKRQRQNNF PMFPSPKAWN FRGRKRKQST QDEDAVSLCS 70 80 90 100 110 120 LDISEPSNKR VKPLSRVTSL ANLIPPVKAT PLKRFSQTLQ RSISFRSESR PDILAPRPWS 130 140 150 160 170 180 RNAAPSSTKR RDSKLWSETF DVCVNQMLTS KEIKRQEAIF ELSQGEEDLI EDLKLAKKAY 190 200 210 220 230 240 HDPMLKLSIM TEQELNQIFG TLDSLIPLHE ELLSQLRDVR KPDGSTEHVG PILVGWLPCL 250 260 270 280 290 300 SSYDSYCSNQ VAAKALLDHK KQDHRVQDFL QRCLESPFSR KLDLWNFLDI PRSRLVKYPL 310 320 330 340 350 360 LLREILRHTP NDNPDQQHLE EAINIIQGIV AEINTKTGES ECRYYKERLL YLEEGQKDSL 370 380 390 400 410 420 IDSSRVLCCH GELKNNRGVK LHVFLFQEVL VITRAVTHNE QLCYQLYRQP IPVKDLLLED 430 440 450 460 470 480 LQDGEVRLGG SLRGAFSNNE RIKNFFRVSF KNGSQSQTHS LQANDTFNKQ QWLNCIRQAK 490 500 510 520 530 540 ETVLCAAGQA GVLDSEGSFL NPTTGSRELQ GETKLEQMDQ SDSESDCSMD TSEVSLDCER 550 MEQTDSSCGN SRHGESNV « Hide
Isoform 3 [UniParc]. Checksum: 64940517719915C0 Show »		532	60,813
10 20 30 40 50 60 MIEVCHHNWL LWLCPSKFGI FMCCLASTTG QMELRRTREP SNKRVKPLSR VTSLANLIPP 70 80 90 100 110 120 VKATPLKRFS QTLQRSISFR SESRPDILAP RPWSRNAAPS STKRRDSKLW SETFDVCVNQ 130 140 150 160 170 180 MLTSKEIKRQ EAIFELSQGE EDLIEDLKLA KKAYHDPMLK LSIMTEQELN QIFGTLDSLI 190 200 210 220 230 240 PLHEELLSQL RDVRKPDGST EHVGPILVGW LPCLSSYDSY CSNQVAAKAL LDHKKQDHRV 250 260 270 280 290 300 QDFLQRCLES PFSRKLDLWN FLDIPRSRLV KYPLLLREIL RHTPNDNPDQ QHLEEAINII 310 320 330 340 350 360 QGIVAEINTK TGESECRYYK ERLLYLEEGQ KDSLIDSSRV LCCHGELKNN RGVKLHVFLF 370 380 390 400 410 420 QEVLVITRAV THNEQLCYQL YRQPIPVKDL LLEDLQDGEV RLGGSLRGAF SNNERIKNFF 430 440 450 460 470 480 RVSFKNGSQS QTHSLQANDT FNKQQWLNCI RQAKETVLCA AGQAGVLDSE GSFLNPTTGS 490 500 510 520 530 RELQGETKLE QMDQSDSESD CSMDTSEVSL DCERMEQTDS SCGNSRHGES NV « Hide
Isoform 4 [UniParc]. Checksum: DF90022C9392296E Show »		324	36,981
10 20 30 40 50 60 MKLPCLSSYD SYCSNQVAAK ALLDHKKQDH RVQDFLQRCL ESPFSRKLDL WNFLDIPRSR 70 80 90 100 110 120 LVKYPLLLRE ILRHTPNDNP DQQHLEEAIN IIQGIVAEIN TKTGESECRY YKERLLYLEE 130 140 150 160 170 180 GQKDSLIDSS RVLCCHGELK NNRGVKLHVF LFQEVLVITR AVTHNEQLCY QLYRQPIPVK 190 200 210 220 230 240 DLLLEDLQDG EVRLGGSLRG AFSNNERIKN FFRVSFKNGS QSQTHSLQAN DTFNKQQWLN 250 260 270 280 290 300 CIRQAKETVL CAAGQAGVLD SEGSFLNPTT GSRELQGETK LEQMDQSDSE SDCSMDTSEV 310 320 SLDCERMEQT DSSCGNSRHG ESNV « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of two novel human RhoGEFs, ARHGEF3 and ARHGEF4, in 3p13-21 and 2q22." Thiesen S., Kuebart S., Ropers H.-H., Nothwang H.G. Biochem. Biophys. Res. Commun. 273:364-369(2000) [PubMed: 10873612] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. Poustka A. Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[3]	Guo J.H., Yu L. Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT VAL-335.
[4]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno F.R. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Brain.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4), VARIANT VAL-335. Tissue: Hippocampus and Uterus.
[6]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 71-526 (ISOFORMS 1/2/3). Tissue: Placenta.
[7]	"XPLN, a guanine nucleotide exchange factor for RhoA and RhoB, but not RhoC." Arthur W.T., Ellerbroek S.M., Der C.J., Burridge K., Wennerberg K. J. Biol. Chem. 277:42964-42972(2002) [PubMed: 12221096] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	AF249744 mRNA. Translation: AAF79954.1. AL136832 mRNA. Translation: CAB66766.1. AF433662 mRNA. Translation: AAP97313.1. AB209661 mRNA. Translation: BAD92898.1. Different initiation. BC054345 mRNA. Translation: AAH54345.2. BC068513 mRNA. Translation: AAH68513.1. Different initiation. BC098122 mRNA. Translation: AAH98122.2. BC098272 mRNA. Translation: AAH98272.2. BC099715 mRNA. Translation: AAH99715.1. BC104723 mRNA. Translation: AAI04724.2. AK024340 mRNA. Translation: BAB14891.1. Different initiation.
RefSeq	NP_001122088.1. NP_062455.1.
UniGene	Hs.476402
3D structure databases
HSSP	HSSP built from PDB template 1KI1 based on UniProtKB Q15811.
ModBase	Search...
Genome annotation databases
Ensembl	ENSG00000163947. Homo sapiens. [Contig view]
GeneID	50650.
KEGG	hsa:50650.
Organism-specific databases
H-InvDB	HIX0003388.
HGNC	HGNC:683. ARHGEF3.
PharmGKB	PA24973.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	Q9NR81.
Enzyme and pathway databases
Reactome	REACT_11044. Signaling by Rho GTPases.
Gene expression databases
ArrayExpress	Q9NR81.
CleanEx	HS_ARHGEF3.
GermOnline	ENSG00000163947. Homo sapiens.
Family and domain databases
InterPro	IPR000219. DH-domain. IPR001331. GDS_CDC24_CS. IPR001849. PH. IPR015721. RhoGEF_like. [Graphical view]
Gene3D	G3DSA:1.20.900.10. RhoGEF. 1 hit.
PANTHER	PTHR22825. RhoGEF_like. 1 hit.
Pfam	PF00169. PH. 1 hit. PF00621. RhoGEF. 1 hit. [Graphical view]
SMART	SM00233. PH. 1 hit. SM00325. RhoGEF. 1 hit. [Graphical view]
PROSITE	PS00741. DH_1. 1 hit. PS50010. DH_2. 1 hit. PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProDom	Q9NR81. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

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Entry information

Entry name

ARHG3_HUMAN

Accession

Primary (citable) accession number: Q9NR81
Secondary accession number(s): Q4FZB6

Q9H7T4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	October 1, 2000
Last modified:	September 2, 2008
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Index of human polymorphisms and disease mutations

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents