Reviewed, UniProtKB/Swiss-Prot Q9NVJ2 (ARL8B_HUMAN)

Names and origin

Protein names

Recommended name:
    ADP-ribosylation factor-like protein 8B
Alternative name(s):
    ADP-ribosylation factor-like protein 10C
    Novel small G protein indispensable for equal chromosome segregation 1

Gene names

Name:	ARL8B
Synonyms:	ARL10C, GIE1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	186 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play a role in lysosomes motility (Ref.6). May play a role in chromosomes segregation (Ref.1).
Subunit structure	Interacts with tubulin.
Subcellular location	Late endosome membrane. Lysosome membrane. Note= According to Ref.1 it localizes with microtubules at the spindle mid-zone during mitosis.
Tissue specificity	Ubiquitously expressed.
Sequence similarities	Belongs to the small GTPase superfamily. Arf family.

Ontologies

Keywords
Cellular component	Endosome Lysosome Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Acetylation
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	chromosome segregation Inferred from mutant phenotype. Source: UniProtKB
Cellular component	cytoplasm Ref.1 Inferred from direct assay. Source: UniProtKB midbody Ref.1 Inferred from direct assay. Source: UniProtKB spindle midzone Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	GDP binding Ref.1 Inferred from direct assay. Source: UniProtKB GTP binding Ref.1 Inferred from direct assay. Source: UniProtKB GTPase activity Ref.1 Non-traceable author statement. Source: UniProtKB alpha-tubulin binding Ref.1 Inferred from direct assay. Source: UniProtKB beta-tubulin binding Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Chain

1 – 186

186

ADP-ribosylation factor-like protein 8B

Regions

Nucleotide binding

29 – 35

7

GTP

Nucleotide binding

71 – 75

5

GTP By similarity

Nucleotide binding

130 – 133

4

GTP

Region

1 – 19

19

Mediates targeting to membranes

Amino acid modifications

Modified residue

1

1

N-acetylmethionine

Experimental info

Mutagenesis

2

1

L → A: Diffuse cytoplasmic distribution and loss of localization to lysosomes. No effect on acetylation

Mutagenesis

2

1

L → F: No effect on localization and acetylation

Mutagenesis

5 – 12

8

ISRLLDWF → ASRALDWA: Diffuse cytoplasmic distribution and loss of localization to lysosomes. No effect on acetylation

Mutagenesis

34

1

T → N: Preferentially binds GDP. Alters chromosome segregation

Mutagenesis

49 – 58

10

Missing: Alters chromosome segregation

Mutagenesis

70

1

W → R: Preferentially binds GTP

Mutagenesis

74 – 85

12

Missing: Alters chromosome segregation

Mutagenesis

75

1

Q → L: Prevents GTP hydrolysis. No effect on localization. Alters lysosomes cellular distribution and motility

Mutagenesis

130

1

N → I: Loss of GTP/GDP-binding. Affects chromosome segregation

Secondary structure

1

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186

Helix Strand Turn

Details...

Beta strand

19 – 26

8

Helix

33 – 40

8

Beta strand

55 – 62

8

Beta strand

65 – 72

8

Helix

76 – 81

6

Helix

82 – 86

5

Beta strand

90 – 97

8

Helix

101 – 103

3

Helix

104 – 115

12

Helix

118 – 120

3

Beta strand

125 – 130

6

Helix

140 – 146

7

Helix

149 – 151

3

Beta strand

157 – 161

5

Turn

164 – 167

4

Helix

170 – 178

9

Sequences

	Sequence		Length	Mass (Da)
	Q9NVJ2-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 5030B01AFD749223	FASTA	186	21,539
	10 20 30 40 50 60 MLALISRLLD WFRSLFWKEE MELTLVGLQY SGKTTFVNVI ASGQFSEDMI PTVGFNMRKV 70 80 90 100 110 120 TKGNVTIKIW DIGGQPRFRS MWERYCRGVN AIVYMIDAAD REKIEASRNE LHNLLDKPQL 130 140 150 160 170 180 QGIPVLVLGN KRDLPNALDE KQLIEKMNLS AIQDREICCY SISCKEKDNI DITLQWLIQH SKSRRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Novel small GTPase subfamily capable of associating with tubulin is required for chromosome segregation." Okai T., Araki Y., Tada M., Tateno T., Kontani K., Katada T. J. Cell Sci. 117:4705-4715(2004) [PubMed: 15331635] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF THR-34; 49-MET--ARG-58; TRP-70; 74-GLY--TYR-85 AND ASN-130, SUBCELLULAR LOCATION, INTERACTION WITH TUBULIN. Tissue: Brain.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Teratocarcinoma.
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryonic carcinoma and Testis.
[5]	Bienvenut W.V. Submitted (JUN-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 69-77; 147-155 AND 166-182, MASS SPECTROMETRY. Tissue: B-cell lymphoma.
[6]	"An N-terminally acetylated Arf-like GTPase is localised to lysosomes and affects their motility." Hofmann I., Munro S. J. Cell Sci. 119:1494-1503(2006) [PubMed: 16537643] [Abstract] Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, ACETYLATION AT MET-1, MUTAGENESIS OF LEU-2; 5-ILE--PHE-12 AND GLN-75.
[7]	"GTP-like conformation of GDP-bound ARL10C GTPase." Structural genomics consortium (SGC) Submitted (AUG-2005) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 18-184 IN COMPLEX WITH GDP.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AB118751 mRNA. Translation: BAD23992.1.
AK001564 mRNA. Translation: BAA91759.1.
CR457264 mRNA. Translation: CAG33545.1.
BC013131 mRNA. Translation: AAH13131.1.
BC063125 mRNA. Translation: AAH63125.1.

RefSeq

NP_060654.1.

UniGene

Hs.250009

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2AL7	X-ray	1.85	A	18-184	[»]

ModBase

Search...

Genome annotation databases

Ensembl

ENSG00000134108. Homo sapiens. [Contig view]

GeneID

55207.

KEGG

hsa:55207.

Organism-specific databases

H-InvDB

HIX0003016.

HGNC

HGNC:25564. ARL8B.

PharmGKB

PA134959531.

GenAtlas

Search...

GeneCards

Search...

GeneLynx

Search...

Phylogenomic databases

HOGENOM

Q9NVJ2.

HOVERGEN

Q9NVJ2.

Gene expression databases

ArrayExpress

Q9NVJ2.

CleanEx

HS_ARL8B.

GermOnline

ENSG00000134108. Homo sapiens.

Family and domain databases

InterPro

IPR006688. ARF.
IPR006689. ARF/SAR.
IPR001806. Ras_trnsfrmng.
IPR005225. Small_GTP_bd.
[Graphical view]

PANTHER

PTHR11711. ARF/SAR. 1 hit.

Pfam

PF00025. Arf. 1 hit.
[Graphical view]

PRINTS

PR00449. RASTRNSFRMNG.
PR00328. SAR1GTPBP.

SMART

SM00177. ARF. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00231. small_GTP. 1 hit.

ProDom

Q9NVJ2.
[Graphical view] [Entries sharing at least one domain]

BLOCKS

Search...

Other Resources

LinkHub

Q9NVJ2.

ProtoNet

Search...

Entry information

Entry name

ARL8B_HUMAN

Accession

Primary (citable) accession number: Q9NVJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	October 1, 2000
Last modified:	July 22, 2008
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)