Reviewed, UniProtKB/Swiss-Prot Q9P2N4 (ATS9_HUMAN)

Names and origin

Protein names

Recommended name:
    A disintegrin and metalloproteinase with thrombospondin motifs 9
      Short name=ADAMTS-9
      Short name=ADAM-TS 9
      Short name=ADAM-TS9
    EC=3.4.24.-

Gene names

Name:	ADAMTS9
Synonyms:	KIAA1312

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1935 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cleaves the large aggregating proteoglycans, aggrecan and versican.
Catalytic activity	Cleaves aggrecan at the 1838-Glu-|-Ala-1839 site and versican at the 1428-Glu-|-Ala-1429 site.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Secreted › extracellular space › extracellular matrixBy similarity.
Tissue specificity	Highly expressed in all fetal tissues. Expressed in a number of adult tissues with highest expression in heart, placenta and skeletal muscle.
Domain	The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix By similarity. The ancillary domains, including the TSRs domain, are required for specific extracellular localization and for its versicanase and aggrecanase activities. The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational modification	The precursor is cleaved by a furin endopeptidase By similarity.
Sequence similarities	Contains 1 disintegrin domain. Contains 1 GON domain. Contains 1 peptidase M12B domain. Contains 15 TSP type-1 domains.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Coding sequence diversity	Alternative splicing
Domain	Repeat Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Cleavage on pair of basic residues Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	glycoprotein catabolic process Ref.1 Traceable author statement. Source: ProtInc multicellular organismal development Ref.1 Traceable author statement. Source: ProtInc
Molecular function	metallopeptidase activity Ref.1 Ref.2 Traceable author statement. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9P2N4-3)

Also known as: ADAMTS-9B;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9P2N4-1)

Also known as: Long;

The sequence of this isoform differs from the canonical sequence as follows:
     1624-1629: CSVTCG → VPSWEL
     1630-1935: Missing.

Notes: May result from the retention of an intron in the cDNA leading to a prematurate stop codon.

Isoform 3 (identifier: Q9P2N4-2)

Also known as: Short;

The sequence of this isoform differs from the canonical sequence as follows:
     1064-1072: CLVTCGKGH → VRWEGCYFP
     1073-1935: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Propeptide	19 – 287	269
	Chain	288 – 1935	1648	A disintegrin and metalloproteinase with thrombospondin motifs 9
Regions
	Domain	293 – 499	207	Peptidase M12B
	Domain	509 – 587	79	Disintegrin
	Domain	588 – 643	56	TSP type-1 1
	Domain	878 – 936	59	TSP type-1 2
	Domain	939 – 997	59	TSP type-1 3
	Domain	998 – 1049	52	TSP type-1 4
	Domain	1052 – 1109	58	TSP type-1 5
	Domain	1110 – 1166	57	TSP type-1 6
	Domain	1182 – 1240	59	TSP type-1 7
	Domain	1241 – 1296	56	TSP type-1 8
	Domain	1328 – 1379	52	TSP type-1 9
	Domain	1382 – 1440	59	TSP type-1 10
	Domain	1441 – 1494	54	TSP type-1 11
	Domain	1497 – 1555	59	TSP type-1 12
	Domain	1556 – 1611	56	TSP type-1 13
	Domain	1612 – 1676	65	TSP type-1 14
	Domain	1677 – 1734	58	TSP type-1 15
	Domain	1735 – 1935	201	GON
	Region	753 – 877	125	Spacer
	Motif	221 – 228	8	Cysteine switch By similarity
	Compositional bias	88 – 96	9	Poly-Ser
	Compositional bias	644 – 752	109	Cys-rich
Sites
	Active site	435	1	By similarity
	Metal binding	223	1	Zinc; in inhibited form By similarity
	Metal binding	434	1	Zinc; catalytic By similarity
	Metal binding	438	1	Zinc; catalytic By similarity
	Metal binding	444	1	Zinc; catalytic By similarity
Amino acid modifications
	Glycosylation	112	1	N-linked (GlcNAc...) Potential
	Glycosylation	135	1	N-linked (GlcNAc...) Potential
	Glycosylation	271	1	N-linked (GlcNAc...) Potential
	Glycosylation	749	1	N-linked (GlcNAc...) Potential
	Glycosylation	840	1	N-linked (GlcNAc...) Potential
	Glycosylation	1213	1	N-linked (GlcNAc...) Potential
	Glycosylation	1267	1	N-linked (GlcNAc...) Potential
	Glycosylation	1788	1	N-linked (GlcNAc...) Potential
	Glycosylation	1806	1	N-linked (GlcNAc...) Potential
	Disulfide bond	412 ↔ 494		By similarity
	Disulfide bond	450 ↔ 478		By similarity
	Disulfide bond	600 ↔ 637		By similarity
	Disulfide bond	604 ↔ 642		By similarity
	Disulfide bond	615 ↔ 627		By similarity
	Disulfide bond	890 ↔ 931		By similarity
	Disulfide bond	894 ↔ 935		By similarity
	Disulfide bond	904 ↔ 918		By similarity
	Disulfide bond	1250 ↔ 1290		By similarity
	Disulfide bond	1254 ↔ 1295		By similarity
	Disulfide bond	1265 ↔ 1278		By similarity
	Disulfide bond	1624 ↔ 1670		By similarity
	Disulfide bond	1628 ↔ 1675		By similarity
	Disulfide bond	1639 ↔ 1659		By similarity
Natural variations
	Alternative sequence	1064 – 1072	9	CLVTCGKGH → VRWEGCYFP in isoform 3.
	Alternative sequence	1073 – 1935	863	Missing in isoform 3.
	Alternative sequence	1624 – 1629	6	CSVTCG → VPSWEL in isoform 2.
	Alternative sequence	1630 – 1935	306	Missing in isoform 2.
Experimental info
	Sequence conflict	46	1	S → G in AAF89106. Ref.1
	Sequence conflict	96	1	P → S in AAF89106. Ref.1
	Sequence conflict	182	1	D → G in AAO15765. Ref.2
	Sequence conflict	367	1	F → L in AAF89106. Ref.1
	Sequence conflict	1117	1	V → G in BAA92550. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (ADAMTS-9B) [UniParc]. Last modified June 16, 2003. Version 3. Checksum: FD3D51E88300A3C6	FASTA	1,935	216,557
	10 20 30 40 50 60 MQFVSWATLL TLLVRDLAEM GSPDAAAAVR KDRLHPRQVK LLETLSEYEI VSPIRVNALG 70 80 90 100 110 120 EPFPTNVHFK RTRRSINSAT DPWPAFASSS SSSTSPQAHY RLSAFGQQFL FNLTANAGFI 130 140 150 160 170 180 APLFTVTLLG TPGVNQTKFY SEEEAELKHC FYKGYVNTNS EHTAVISLCS GMLGTFRSHD 190 200 210 220 230 240 GDYFIEPLQS MDEQEDEEEQ NKPHIIYRRS APQREPSTGR HACDTSEHKN RHSKDKKKTR 250 260 270 280 290 300 ARKWGERINL AGDVAALNSG LATEAFSAYG NKTDNTREKR THRRTKRFLS YPRFVEVLVV 310 320 330 340 350 360 ADNRMVSYHG ENLQHYILTL MSIVASIYKD PSIGNLINIV IVNLIVIHNE QDGPSISFNA 370 380 390 400 410 420 QTTLKNFCQW QHSKNSPGGI HHDTAVLLTR QDICRAHDKC DTLGLAELGT ICDPYRSCSI 430 440 450 460 470 480 SEDSGLSTAF TIAHELGHVF NMPHDDNNKC KEEGVKSPQH VMAPTLNFYT NPWMWSKCSR 490 500 510 520 530 540 KYITEFLDTG YGECLLNEPE SRPYPLPVQL PGILYNVNKQ CELIFGPGSQ VCPYMMQCRR 550 560 570 580 590 600 LWCNNVNGVH KGCRTQHTPW ADGTECEPGK HCKYGFCVPK EMDVPVTDGS WGSWSPFGTC 610 620 630 640 650 660 SRTCGGGIKT AIRECNRPEP KNGGKYCVGR RMKFKSCNTE PCLKQKRDFR DEQCAHFDGK 670 680 690 700 710 720 HFNINGLLPN VRWVPKYSGI LMKDRCKLFC RVAGNTAYYQ LRDRVIDGTP CGQDTNDICV 730 740 750 760 770 780 QGLCRQAGCD HVLNSKARRD KCGVCGGDNS SCKTVAGTFN TVHYGYNTVV RIPAGATNID 790 800 810 820 830 840 VRQHSFSGET DDDNYLALSS SKGEFLLNGN FVVTMAKREI RIGNAVVEYS GSETAVERIN 850 860 870 880 890 900 STDRIEQELL LQVLSVGKLY NPDVRYSFNI PIEDKPQQFY WNSHGPWQAC SKPCQGERKR 910 920 930 940 950 960 KLVCTRESDQ LTVSDQRCDR LPQPGHITEP CGTDCDLRWH VASRSECSAQ CGLGYRTLDI 970 980 990 1000 1010 1020 YCAKYSRLDG KTEKVDDGFC SSHPKPSNRE KCSGECNTGG WRYSAWTECS KSCDGGTQRR 1030 1040 1050 1060 1070 1080 RAICVNTRND VLDDSKCTHQ EKVTIQRCSE FPCPQWKSGD WSECLVTCGK GHKHRQVWCQ 1090 1100 1110 1120 1130 1140 FGEDRLNDRM CDPETKPTSM QTCQQPECAS WQAGPWVQCS VTCGQGYQLR AVKCIIGTYM 1150 1160 1170 1180 1190 1200 SVVDDNDCNA ATRPTDTQDC ELPSCHPPPA APETRRSTYS APRTQWRFGS WTPCSATCGK 1210 1220 1230 1240 1250 1260 GTRMRYVSCR DENGSVADES ACATLPRPVA KEECSVTPCG QWKALDWSSC SVTCGQGRAT 1270 1280 1290 1300 1310 1320 RQVMCVNYSD HVIDRSECDQ DYIPETDQDC SMSPCPQRTP DSGLAQHPFQ NEDYRPRSAS 1330 1340 1350 1360 1370 1380 PSRTHVLGGN QWRTGPWGAC SSTCAGGSQR RVVVCQDENG YTANDCVERI KPDEQRACES 1390 1400 1410 1420 1430 1440 GPCPQWAYGN WGECTKLCGG GIRTRLVVCQ RSNGERFPDL SCEILDKPPD REQCNTHACP 1450 1460 1470 1480 1490 1500 HDAAWSTGPW SSCSVSCGRG HKQRNVYCMA KDGSHLESDY CKHLAKPHGH RKCRGGRCPK 1510 1520 1530 1540 1550 1560 WKAGAWSQCS VSCGRGVQQR HVGCQIGTHK IARETECNPY TRPESERDCQ GPRCPLYTWR 1570 1580 1590 1600 1610 1620 AEEWQECTKT CGEGSRYRKV VCVDDNKNEV HGARCDVSKR PVDRESCSLQ PCEYVWITGE 1630 1640 1650 1660 1670 1680 WSECSVTCGK GYKQRLVSCS EIYTGKENYE YSYQTTINCP GTQPPSVHPC YLRECPVSAT 1690 1700 1710 1720 1730 1740 WRVGNWGSCS VSCGVGVMQR SVQCLTNEDQ PSHLCHTDLK PEERKTCRNV YNCELPQNCK 1750 1760 1770 1780 1790 1800 EVKRLKGASE DGEYFLMIRG KLLKIFCAGM HSDHPKEYVT LVHGDSENFS EVYGHRLHNP 1810 1820 1830 1840 1850 1860 TECPYNGSRR DDCQCRKDYT AAGFSSFQKI RIDLTSMQII TTDLQFARTS EGHPVPFATA 1870 1880 1890 1900 1910 1920 GDCYSAAKCP QGRFSINLYG TGLSLTESAR WISQGNYAVS DIKKSPDGTR VVGKCGGYCG 1930 KCTPSSGTGL EVRVL
	Isoform 2 (Long) [UniParc]. Checksum: 5227DFD03ED67249		1,629	182,732
	10 20 30 40 50 60 MQFVSWATLL TLLVRDLAEM GSPDAAAAVR KDRLHPRQVK LLETLSEYEI VSPIRVNALG 70 80 90 100 110 120 EPFPTNVHFK RTRRSINSAT DPWPAFASSS SSSTSPQAHY RLSAFGQQFL FNLTANAGFI 130 140 150 160 170 180 APLFTVTLLG TPGVNQTKFY SEEEAELKHC FYKGYVNTNS EHTAVISLCS GMLGTFRSHD 190 200 210 220 230 240 GDYFIEPLQS MDEQEDEEEQ NKPHIIYRRS APQREPSTGR HACDTSEHKN RHSKDKKKTR 250 260 270 280 290 300 ARKWGERINL AGDVAALNSG LATEAFSAYG NKTDNTREKR THRRTKRFLS YPRFVEVLVV 310 320 330 340 350 360 ADNRMVSYHG ENLQHYILTL MSIVASIYKD PSIGNLINIV IVNLIVIHNE QDGPSISFNA 370 380 390 400 410 420 QTTLKNFCQW QHSKNSPGGI HHDTAVLLTR QDICRAHDKC DTLGLAELGT ICDPYRSCSI 430 440 450 460 470 480 SEDSGLSTAF TIAHELGHVF NMPHDDNNKC KEEGVKSPQH VMAPTLNFYT NPWMWSKCSR 490 500 510 520 530 540 KYITEFLDTG YGECLLNEPE SRPYPLPVQL PGILYNVNKQ CELIFGPGSQ VCPYMMQCRR 550 560 570 580 590 600 LWCNNVNGVH KGCRTQHTPW ADGTECEPGK HCKYGFCVPK EMDVPVTDGS WGSWSPFGTC 610 620 630 640 650 660 SRTCGGGIKT AIRECNRPEP KNGGKYCVGR RMKFKSCNTE PCLKQKRDFR DEQCAHFDGK 670 680 690 700 710 720 HFNINGLLPN VRWVPKYSGI LMKDRCKLFC RVAGNTAYYQ LRDRVIDGTP CGQDTNDICV 730 740 750 760 770 780 QGLCRQAGCD HVLNSKARRD KCGVCGGDNS SCKTVAGTFN TVHYGYNTVV RIPAGATNID 790 800 810 820 830 840 VRQHSFSGET DDDNYLALSS SKGEFLLNGN FVVTMAKREI RIGNAVVEYS GSETAVERIN 850 860 870 880 890 900 STDRIEQELL LQVLSVGKLY NPDVRYSFNI PIEDKPQQFY WNSHGPWQAC SKPCQGERKR 910 920 930 940 950 960 KLVCTRESDQ LTVSDQRCDR LPQPGHITEP CGTDCDLRWH VASRSECSAQ CGLGYRTLDI 970 980 990 1000 1010 1020 YCAKYSRLDG KTEKVDDGFC SSHPKPSNRE KCSGECNTGG WRYSAWTECS KSCDGGTQRR 1030 1040 1050 1060 1070 1080 RAICVNTRND VLDDSKCTHQ EKVTIQRCSE FPCPQWKSGD WSECLVTCGK GHKHRQVWCQ 1090 1100 1110 1120 1130 1140 FGEDRLNDRM CDPETKPTSM QTCQQPECAS WQAGPWVQCS VTCGQGYQLR AVKCIIGTYM 1150 1160 1170 1180 1190 1200 SVVDDNDCNA ATRPTDTQDC ELPSCHPPPA APETRRSTYS APRTQWRFGS WTPCSATCGK 1210 1220 1230 1240 1250 1260 GTRMRYVSCR DENGSVADES ACATLPRPVA KEECSVTPCG QWKALDWSSC SVTCGQGRAT 1270 1280 1290 1300 1310 1320 RQVMCVNYSD HVIDRSECDQ DYIPETDQDC SMSPCPQRTP DSGLAQHPFQ NEDYRPRSAS 1330 1340 1350 1360 1370 1380 PSRTHVLGGN QWRTGPWGAC SSTCAGGSQR RVVVCQDENG YTANDCVERI KPDEQRACES 1390 1400 1410 1420 1430 1440 GPCPQWAYGN WGECTKLCGG GIRTRLVVCQ RSNGERFPDL SCEILDKPPD REQCNTHACP 1450 1460 1470 1480 1490 1500 HDAAWSTGPW SSCSVSCGRG HKQRNVYCMA KDGSHLESDY CKHLAKPHGH RKCRGGRCPK 1510 1520 1530 1540 1550 1560 WKAGAWSQCS VSCGRGVQQR HVGCQIGTHK IARETECNPY TRPESERDCQ GPRCPLYTWR 1570 1580 1590 1600 1610 1620 AEEWQECTKT CGEGSRYRKV VCVDDNKNEV HGARCDVSKR PVDRESCSLQ PCEYVWITGE WSEVPSWEL
	Isoform 3 (Short) [UniParc]. Checksum: 8B9E86DFA0B1300A		1,072	120,727
	10 20 30 40 50 60 MQFVSWATLL TLLVRDLAEM GSPDAAAAVR KDRLHPRQVK LLETLSEYEI VSPIRVNALG 70 80 90 100 110 120 EPFPTNVHFK RTRRSINSAT DPWPAFASSS SSSTSPQAHY RLSAFGQQFL FNLTANAGFI 130 140 150 160 170 180 APLFTVTLLG TPGVNQTKFY SEEEAELKHC FYKGYVNTNS EHTAVISLCS GMLGTFRSHD 190 200 210 220 230 240 GDYFIEPLQS MDEQEDEEEQ NKPHIIYRRS APQREPSTGR HACDTSEHKN RHSKDKKKTR 250 260 270 280 290 300 ARKWGERINL AGDVAALNSG LATEAFSAYG NKTDNTREKR THRRTKRFLS YPRFVEVLVV 310 320 330 340 350 360 ADNRMVSYHG ENLQHYILTL MSIVASIYKD PSIGNLINIV IVNLIVIHNE QDGPSISFNA 370 380 390 400 410 420 QTTLKNFCQW QHSKNSPGGI HHDTAVLLTR QDICRAHDKC DTLGLAELGT ICDPYRSCSI 430 440 450 460 470 480 SEDSGLSTAF TIAHELGHVF NMPHDDNNKC KEEGVKSPQH VMAPTLNFYT NPWMWSKCSR 490 500 510 520 530 540 KYITEFLDTG YGECLLNEPE SRPYPLPVQL PGILYNVNKQ CELIFGPGSQ VCPYMMQCRR 550 560 570 580 590 600 LWCNNVNGVH KGCRTQHTPW ADGTECEPGK HCKYGFCVPK EMDVPVTDGS WGSWSPFGTC 610 620 630 640 650 660 SRTCGGGIKT AIRECNRPEP KNGGKYCVGR RMKFKSCNTE PCLKQKRDFR DEQCAHFDGK 670 680 690 700 710 720 HFNINGLLPN VRWVPKYSGI LMKDRCKLFC RVAGNTAYYQ LRDRVIDGTP CGQDTNDICV 730 740 750 760 770 780 QGLCRQAGCD HVLNSKARRD KCGVCGGDNS SCKTVAGTFN TVHYGYNTVV RIPAGATNID 790 800 810 820 830 840 VRQHSFSGET DDDNYLALSS SKGEFLLNGN FVVTMAKREI RIGNAVVEYS GSETAVERIN 850 860 870 880 890 900 STDRIEQELL LQVLSVGKLY NPDVRYSFNI PIEDKPQQFY WNSHGPWQAC SKPCQGERKR 910 920 930 940 950 960 KLVCTRESDQ LTVSDQRCDR LPQPGHITEP CGTDCDLRWH VASRSECSAQ CGLGYRTLDI 970 980 990 1000 1010 1020 YCAKYSRLDG KTEKVDDGFC SSHPKPSNRE KCSGECNTGG WRYSAWTECS KSCDGGTQRR 1030 1040 1050 1060 1070 RAICVNTRND VLDDSKCTHQ EKVTIQRCSE FPCPQWKSGD WSEVRWEGCY FP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ADAMTS 9, a novel member of the ADAM-TS/Metallospondin gene family." Clark M.E., Kelner G.S., Turbeville L.A., Boyer A., Arden K.A., Maki R.A. Genomics 67:343-350(2000) [PubMed: 10936055] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Tissue: Fetus.
[2]	"Characterization of ADAMTS-9 and ADAMTS-20 as a distinct ADAMTS subfamily related to Caenorhabditis elegans GON-1." Somerville R.P., Longpre J.-M., Jungers K.A., Engle J.M., Ross M., Evanko S., Wight T.N., Leduc R., Apte S.S. J. Biol. Chem. 278:9503-9513(2003) [PubMed: 12514189] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
[3]	"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O. DNA Res. 7:65-73(2000) [PubMed: 10718198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 159-1935 (ISOFORM 2). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF261918 mRNA. Translation: AAF89106.1. AF488803 mRNA. Translation: AAO15765.1. AB037733 mRNA. Translation: BAA92550.1.