Reviewed, UniProtKB/Swiss-Prot Q9PI05 (SYA_CAMJE)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Cj0506

Organism

Campylobacter jejuni [Complete proteome] [HAMAP]

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	842 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 842	842	Alanyl-tRNA synthetase

Sequences

	Sequence		Length	Mass (Da)
	Q9PI05-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: F571EA5A2B60A8B7	FASTA	842	94,906
	10 20 30 40 50 60 MDIRKAYLDF FASKGHEITP SSPLVPDDAT LLFTNAGMVP FKSIFTGEIP RPNPPRKTSC 70 80 90 100 110 120 QTCIRAGGKH NDLDNVGYTA RHHTFFEMLG NFSFGDYFKE QAIAYAWEFV TEVLKLPKDR 130 140 150 160 170 180 LYVTVHENDD EAFNLWQKHI QKERIYKFGD KDNFWQMGDT GPCGPCSEIF YDQGQEHFNS 190 200 210 220 230 240 SEDYMGGDGD RFLEIWNLVF MQYERSADGV LSPLPKPSID TGMGLERVTA IKEGKFSNFD 250 260 270 280 290 300 SSLFMPIINE ISKLCNKTYV YESGASFRVI ADHIRSSVFL LAQGVSFDKE GRGYVLRRIL 310 320 330 340 350 360 RRALRHGYLL GFKQAFMYKL VDVVCDLMGG HYTYLNEKKD FIKEQIRLEE ERFLSTIENG 370 380 390 400 410 420 IEIFNEELKN TKEIFSGEVA FKLYDTYGFP LDLTADMLRE KNLKVDEEKF ELFMNEQKAR 430 440 450 460 470 480 AKASWKGSGD KTASGDFKNL LEKFGENHFV GYEKAECESK ILALLDEDFK EVSTLKDAGW 490 500 510 520 530 540 VMLENTPFYA TSGGQSADSG FIAKREVLDT QKFFNLNLSF IKAGEELKVN DIVHARIDTE 550 560 570 580 590 600 KREQIARHHS ATHLLHHALR EILGSHVSQA GSLVESNKLR FDFTHHKALN KEELESIEKR 610 620 630 640 650 660 VNKMIINSSE AILENMPLEE AKKSGAIALF NEKYQGNVRV LTLGESKELC GGTHVKNTAQ 670 680 690 700 710 720 IGSFYIVKES GVSAGVRRIE AVVSKAALEF VKNQLEELSK VKDELKNNDI LSGIKKLKNE 730 740 750 760 770 780 ILSLKNELKN SSKTELDSKN IQGVEICVKR IDNGDIKAMI DDFKNKFAKA VILLIQVKDE 790 800 810 820 830 840 KITLAAGVKD VPLKAGALVK EAAQILGGNG GGRDDFATAG GKDLSKINEA LKQSLETIEK AL

References

[1]

"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL111168 Genomic DNA. Translation: CAL34653.1.
PIR	C81396.
3D structure databases
ModBase	Search...
Genome annotation databases
GenomeReviews	Gene locus Cj0506 in contig AL111168_GR.
KEGG	cje:Cj0506.
Organism-specific databases
CMR	Search...
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ0506-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	Q9PI05. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_CAMJE

Accession

Primary (citable) accession number: Q9PI05
Secondary accession number(s): Q0PB09

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2002
Last sequence update:	October 1, 2000
Last modified:	July 22, 2008
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)