Reviewed, UniProtKB/Swiss-Prot Q9R1L5 (MAST1_MOUSE)

Names and origin

Protein names

Recommended name:
    Microtubule-associated serine/threonine-protein kinase 1
    EC=2.7.11.1
Alternative name(s):
    Syntrophin-associated serine/threonine-protein kinase

Gene names

Name:	Mast1
Synonyms:	Kiaa0973, Sast

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	1570 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Subunit structure	Part of a low affinity complex that associates with, but is distinct from, the post-synaptic density. Interacts with SNTB2.
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm › cytoskeleton. Note= Colocalizes with syntrophins at the cell membrane.
Tissue specificity	Expressed in the vascular endothelium and in proximity to neuronal nuclei throughout the cortex and cerebellum. Also detected in ependymal cells, the choroid plexus, in developing spermatid acrosomes and in both the cell bodies and axonal processes of motor neurons. Observed as punctate clusters in the processes of interneurons and along the cell body periphery.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. Contains 1 AGC-kinase C-terminal domain. Contains 1 PDZ (DHR) domain. Contains 1 protein kinase domain.
Sequence caution	The sequence AAD50548.1 differs from that shown. Reason: Frameshift at several positions. The sequence BAC65693.3 differs from that shown. Reason: Miscellaneous discrepancy. The sequence differs from that shown because it seems to be derived from a pre-mRNA.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Cytoskeleton Membrane
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cytoskeleton organization and biogenesis Inferred from direct assay. Source: UniProtKB protein amino acid phosphorylation Inferred from sequence or structural similarity. Source: UniProtKB protein kinase cascade Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	membrane Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	ATP binding Inferred from sequence or structural similarity. Source: UniProtKB magnesium ion binding Inferred from sequence or structural similarity. Source: UniProtKB protein binding Ref.1 Inferred from physical interaction. Source: UniProtKB protein serine/threonine kinase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PTEN	P60484	2	EBI-491771,EBI-696162	From a different organism.
Sntb2	Q61235	1	EBI-491771,EBI-295974

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 1570	1570	Microtubule-associated serine/threonine-protein kinase 1
Regions
	Domain	375 – 648	274	Protein kinase
	Domain	649 – 720	72	AGC-kinase C-terminal
	Domain	968 – 1056	89	PDZ
	Nucleotide binding	381 – 389	9	ATP By similarity
Sites
	Active site	498	1	Proton acceptor By similarity
	Binding site	404	1	ATP By similarity
Amino acid modifications
	Modified residue	161	1	Phosphoserine By similarity
	Modified residue	163	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	158	1	R → P in AAD50548. Ref.1
	Sequence conflict	197 – 198	2	LR → P in AAD50548. Ref.1
	Sequence conflict	647	1	S → T in AAD50548. Ref.1
	Sequence conflict	672	1	D → E in AAD50548. Ref.1
	Sequence conflict	1190	1	H → L in AAD50548. Ref.1
	Sequence conflict	1219 – 1250	32	SPPPL…PVVRP → ARRRCQATRWAAHIHAELPC QTTYIATCVRR in AAD50548. Ref.1
	Sequence conflict	1454	1	L → V in AAD50548. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9R1L5-1 [UniParc]. Last modified September 11, 2007. Version 3. Checksum: A8F9C868CF241486	FASTA	1,570	170,997
	10 20 30 40 50 60 MSDSLWTALS NFSMPSFPGG SMFRRTKSCR TSNRKSLILT STSPTLPRPH SPLPGHLGSS 70 80 90 100 110 120 PLDSPRNFSP NTPAHFSFAS SRRADGRRWS LASLPSSGYG TNTPSSTVSS SCSSQERLHQ 130 140 150 160 170 180 LPYQPTVDEL HFLSKHFGST ESITDEDGGR RSPAVRPRSR SLSPGRSPSS YDNEIVMMNH 190 200 210 220 230 240 VYKERFPKAT AQMEEKLRDF ARAYEPDSVL PLADGVLSFI HHQIIELARD CLTKSRDGLI 250 260 270 280 290 300 TTVYFYELQE NLEKLLQDAY ERSESLEVAF VTQLVKKLLI IISRPARLLE CLEFNPEEFY 310 320 330 340 350 360 HLLEAAEGHA KEGHLVKTDI PRYIIRQLGL TRDPFPDVVR LEEQDSGGSN TPEQDDTSEG 370 380 390 400 410 420 RSSTSKAKKP PGESDFDTIK LISNGAYGAV YLVRHRDTRQ RFAMKKINKQ NLILRNQIQQ 430 440 450 460 470 480 AFVERDILTF AENPFVVGMF CSFETRRHLC MVMEYVEGGD CATLLKNIGA LPVEMARMYF 490 500 510 520 530 540 AETVLALEYL HNYGIVHRDL KPDNLLITSM GHIKLTDFGL SKMGLMSLTT NLYEGHIEKD 550 560 570 580 590 600 AREFLDKQVC GTPEYIAPEV ILRQGYGKPV DWWAMGIILY EFLVGCVPFF GDTPEELFGQ 610 620 630 640 650 660 VISDDILWPE GDEALPTDAQ LLISSLLQTN PLVRLGAGGA FEVKQHSFFR DLDWTGLLRQ 670 680 690 700 710 720 KAEFIPHLES EDDTSYFDTR SDRYHHVNSY DEDDTTEEEP VEIRQFSSCS PRFSKVYSSM 730 740 750 760 770 780 EQLSQHEPKT PVSASGASKR DPSAKGPEEK VAGKREGLGG LTLREKTWRG GSPEIKRFSA 790 800 810 820 830 840 SEASFLEGEA SPPLGARRRF SALLEPSRFT APQEDEDEAR LRRPPRPSSD PPSSLDTRVP 850 860 870 880 890 900 KEAVQGEGTS TPGEPEATER SHPGDLGPPS KDGDPSGPRA TNDLVLRRAR HQQLSGDLAV 910 920 930 940 950 960 EKRPSRTGGK VIKSASATAL SVMIPAVDPH GGSPLASPMS PRSLSSNPSS RDSSPSRDYS 970 980 990 1000 1010 1020 PAVSGLRSPI TIQRSGKKYG FTLRAIRVYM GDSDVYSVHH IVWHVEEGGP AQEAGLCAGD 1030 1040 1050 1060 1070 1080 LITHVNGEPV HGMVHPEVVE LILKSGNKVA VTTTPFENTS IRIGPARRSS YKAKMARRNK 1090 1100 1110 1120 1130 1140 RPSAKDGQES KKRSSLFRKI TKQSNLLHTS RSLSSLNRSL SSSDSLPGSP THGLPARSPT 1150 1160 1170 1180 1190 1200 HSYRSTPDSA YLGASSQSSS PASSTPNSPA SSASHHIRPS TLHGLSPKLH RQYRSARCKS 1210 1220 1230 1240 1250 1260 AGNIPLSPLA HTPSPTQASP PPLPGHTVGS SHTTQSFPAK LHSSPPVVRP RPKSAEPPRS 1270 1280 1290 1300 1310 1320 PLLKRVQSAE KLGASLGADK KGALRKHSLE VGHPDFRKDF HGELALHSLA ESDGETPPIE 1330 1340 1350 1360 1370 1380 GPGATRQVAV RRLGRQESPL SLGADPLLPD GVQRPMASSK EDSAGGTEAC TPPRATTPGS 1390 1400 1410 1420 1430 1440 RTLERDSGCT RHQSVQTEDG PGGVARALAK AALSPVQEHE TGRRSSSGEA GTPPVPIVVE 1450 1460 1470 1480 1490 1500 PARPGVKTQA PQPLGTDSKG LKEPVAQMPL MPDAPRGRER WVLEEVEERT TLSGLRSKPA 1510 1520 1530 1540 1550 1560 SPKLSPDPQT PTLVPTKNVP RSAAPSVPPA SLMVPGTKPE AGLNSRCPAE AVTPAGLTKT 1570 GAPSPASLGP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Interactions between beta 2-syntrophin and a family of microtubule-associated serine/threonine kinases." Lumeng C., Phelps S., Crawford G.E., Walden P.D., Barald K., Chamberlain J.S. Nat. Neurosci. 2:611-617(1999) [PubMed: 10404183] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH SNTB2. Strain: C57BL/10.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[3]	"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H. DNA Res. 10:35-48(2003) [PubMed: 12693553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 337-1570. Tissue: Brain.
[4]	Okazaki N., Kikuno R., Nagase T., Ohara O., Koga H. Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF077818 mRNA. Translation: AAD50548.1. Frameshift. BC054524 mRNA. Translation: AAH54524.1. AK122411 Other RNA. Translation: BAC65693.3. Sequence problems.
RefSeq	NP_064329.2.
UniGene	Mm.154614
3D structure databases
HSSP	HSSP built from PDB template 1MRY based on UniProtKB P31751.
SMR	Q9R1L5. Positions 187-286.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9R1L5.
PTM databases
PhosphoSite	Q9R1L5.
Genome annotation databases
Ensembl	ENSMUSG00000053693. Mus musculus. [Contig view]
GeneID	56527.
KEGG	mmu:56527.
Organism-specific databases
MGI	MGI:1861901. Mast1.
Rouge	Search...
Phylogenomic databases
HOGENOM	Q9R1L5.
HOVERGEN	Q9R1L5.
Gene expression databases
CleanEx	MM_MAST1.
GermOnline	ENSMUSG00000053693. Mus musculus.
Family and domain databases
InterPro	IPR001478. PDZ. IPR000961. Pkinase_C. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00595. PDZ. 1 hit. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00228. PDZ. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS50106. PDZ. 1 hit. PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

MAST1_MOUSE

Accession

Primary (citable) accession number: Q9R1L5
Secondary accession number(s): Q7TQG9, Q80TN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	September 11, 2007
Last modified:	July 22, 2008
This is version 64 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)