Reviewed, UniProtKB/Swiss-Prot Q9RS27 (SYA_DEIRA)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name(s)=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	DR_2300

Organism

Deinococcus radiodurans [Complete proteome] [HAMAP]

Taxonomic identifier

1299 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Deinococcales › Deinococcaceae › Deinococcus

Protein attributes

Sequence length	890 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 890	890	Alanyl-tRNA synthetase

Sequences

	Sequence		Length	Mass (Da)
	Q9RS27-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: FABE000AE34DAEFB	FASTA	890	95,987
	10 20 30 40 50 60 MTAPLTTAEI RERYLYFFET KGHLRLPSHS TIAPDPTTLF TVAGMQPFKE NFMGAPAVFD 70 80 90 100 110 120 GQPSKRVTTA QKCVRVGDIE NVGRTRRHLS LFEMMGNFSF GDYFKRDAIH WAWEFLTGPE 130 140 150 160 170 180 WMGMDKDKMY VTVYKDDDEA FGYWTQDIGL PAEHIHRFDA DENFWPANAP LEGPNGPCGP 190 200 210 220 230 240 CSEIYYDRGE NYGDDTWGDY YQTRESARFL EVWNLVFPQY DRQDLDASGQ PVLKDLPFKN 250 260 270 280 290 300 IDTGMGLERV ASVVQDVPDF YSNDVFKPIV ERVAELSGKP YEGETSVSHR VVAEHIRSVS 310 320 330 340 350 360 MIVADGTAFS NTGRGYTARK IMRRAIRHGY MLGLREPQLY RLVELVVDSM GGAYPELQHN 370 380 390 400 410 420 QSRVEASVRA EEEQFLKTLE GGIKRLGGLL SGMEKGSTLV GKDAFELYDT YGFPVDLTKE 430 440 450 460 470 480 IAEEYGVSVD EAGYAESLEH AQEIARAGSK YGKSELFGGH QEALDGLPAT QFVGYDQTSG 490 500 510 520 530 540 DGQVLALLSG GERLSHLPAG SEATVVLSQT PFYGEGGGEV GDTGRLEWDG GAGIVRDTQK 550 560 570 580 590 600 TKQGVFLHDV LVEQGELKEG TRVRGVVSPE RAAIQRHHTA THLLHAALRA VLGGGVQQKG 610 620 630 640 650 660 SRVAADQLRF DFSHGAAMSA EEIAQVETLV SRWVSANFPV SWREMPIAEA KAAGATALFG 670 680 690 700 710 720 EKYGDVVRVV RVEGDVDFGG HAVSSMELCG GAHVSRTGDI GAFVIVSDEN VAAGVRRIEA 730 740 750 760 770 780 LAGEQATAWL RGRLNASAKA AALLNTSPEG LEERVSGLQG QLKAAEKETA QARRQLAEAQ 790 800 810 820 830 840 MGGGGSAAQT RELGGFKVAS LKLSGIEGNE LRGAADKLLD QSGADLVVIA SDKGLVVKAT 850 860 870 880 890 KDAVAKGAHA GQLVSKLAAA GGGKGGGRPD MAQAGITDAA GALGALDTAF

References

[1]

"Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1." White O., Eisen J.A., Heidelberg J.F., Hickey E.K., Peterson J.D., Dodson R.J., Haft D.H., Gwinn M.L., Nelson W.C., Richardson D.L., Moffat K.S., Qin H., Jiang L., Pamphile W., Crosby M., Shen M., Vamathevan J.J., Lam P. , McDonald L.A., Utterback T.R., Zalewski C., Makarova K.S., Aravind L., Daly M.J., Minton K.W., Fleischmann R.D., Ketchum K.A., Nelson K.E., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Science 286:1571-1577(1999) [PubMed: 10567266] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13939 / DSM 20539 / IFO 15346 / LMG 4051 / NCIB 9279 / R1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000513 Genomic DNA. Translation: AAF11848.1.
PIR	F75289.
RefSeq	NP_296021.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1798278.
GenomeReviews	Gene locus DR_2300 in contig AE000513_GR.
KEGG	dra:DR_2300.
NMPDR	fig|243230.1.peg.2480.
TIGR	DR_2300.
Phylogenomic databases
HOGENOM	Q9RS27.
Enzyme and pathway databases
BioCyc	DRAD243230:DR_2300-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	Q9RS27. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_DEIRA

Accession

Primary (citable) accession number: Q9RS27

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2002
Last sequence update:	May 1, 2000
Last modified:	July 22, 2008
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)