Reviewed, UniProtKB/Swiss-Prot Q9SSX0 (RAC1_ORYSJ)

Names and origin

Protein names

Recommended name:
    Rac-like GTP-binding protein 1
Alternative name(s):
    OsRac1

Gene names

Name:	RAC1
Ordered Locus Names:	Os01g0229400, LOC_Os01g12900

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	214 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Small GTPase playing a general role in disease resistance signaling pathway. Acts downstream of heterotrimeric G protein alpha subunit. Regulates cell death and reactive oxygen species production, probably through NADPH oxidase. Also involved in sphingolipid elicitor (SE)-dependent defense signaling. Activates phytoalexin production and alters defense-related genes. Down-regulates metallothionein 2b, a reactive oxygen scavenger.
Subunit structure	May interact with MPK1/MAPK6.
Subcellular location	Cytoplasm. Membrane; Peripheral membrane protein. Note= Associated with the membrane when activated.
Post-translational modification	May be palmitoylated.
Sequence similarities	Belongs to the small GTPase superfamily. Rho family.

Ontologies

Keywords
Biological process	Apoptosis Plant defense
Cellular component	Cytoplasm Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Palmitate
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 214	214	Rac-like GTP-binding protein 1
Regions
	Nucleotide binding	17 – 24	8	GTP By similarity
	Nucleotide binding	64 – 68	5	GTP By similarity
	Nucleotide binding	122 – 125	4	GTP By similarity
	Motif	39 – 47	9	Effector region Potential
Experimental info
	Mutagenesis	19	1	G → V: Constitutively active
	Mutagenesis	24	1	T → N: Constitutively inactive
	Mutagenesis	212	1	C → S: Loss of membrane localization

Sequences

	Sequence		Length	Mass (Da)
	Q9SSX0-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: AFBAF00E593C0152	FASTA	214	23,717
	10 20 30 40 50 60 MSSAAAATRF IKCVTVGDGA VGKTCMLICY TCNKFPTDYI PTVFDNFSAN VSVDGSVVNL 70 80 90 100 110 120 GLWDTAGQED YSRLRPLSYR GADVFILSFS LISRASYENV QKKWMPELRR FAPGVPVVLV 130 140 150 160 170 180 GTKLDLREDR AYLADHPASS IITTEQGEEL RKLIGAVAYI ECSSKTQRNI KAVFDTAIKV 190 200 210 VLQPPRHKDV TRKKLQSSSN RPVRRYFCGS ACFA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The small GTP-binding protein Rac is a regulator of cell death in plants." Kawasaki T., Henmi K., Ono E., Hatakeyama S., Iwano M., Satoh H., Shimamoto K. Proc. Natl. Acad. Sci. U.S.A. 96:10922-10926(1999) [PubMed: 10485927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF GLY-19 AND THR-24.
[2]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Essential role of the small GTPase Rac in disease resistance of rice." Ono E., Wong H.L., Kawasaki T., Hasegawa M., Kodama O., Shimamoto K. Proc. Natl. Acad. Sci. U.S.A. 98:759-764(2001) [PubMed: 11149940] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-212.
[4]	"The heterotrimeric G protein alpha subunit acts upstream of the small GTPase Rac in disease resistance of rice." Suharsono U., Fujisawa Y., Kawasaki T., Iwasaki Y., Satoh H., Shimamoto K. Proc. Natl. Acad. Sci. U.S.A. 99:13307-13312(2002) [PubMed: 12237405] [Abstract] Cited for: FUNCTION.
[5]	"Down-regulation of metallothionein, a reactive oxygen scavenger, by the small GTPase OsRac1 in rice." Wong H.L., Sakamoto T., Kawasaki T., Umemura K., Shimamoto K. Plant Physiol. 135:1447-1456(2004) [PubMed: 15220467] [Abstract] Cited for: FUNCTION.
[6]	"A sphingolipid elicitor-inducible mitogen-activated protein kinase is regulated by the small GTPase OsRac1 and heterotrimeric G-protein in rice." Lieberherr D., Thao N.P., Nakashima A., Umemura K., Kawasaki T., Shimamoto K. Plant Physiol. 138:1644-1652(2005) [PubMed: 15951489] [Abstract] Cited for: INTERACTION WITH MPK1/MAPK6.
[7]	"RNA silencing of single and multiple members in a gene family of rice." Miki D., Itoh R., Shimamoto K. Plant Physiol. 138:1903-1913(2005) [PubMed: 16172097] [Abstract] Cited for: NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB029508 mRNA. Translation: BAA84492.1. AP001859 Genomic DNA. No translation available.
RefSeq	NP_001042486.1.
UniGene	Os.12209
3D structure databases
HSSP	HSSP built from PDB template 1AM4 based on UniProtKB P60953.
SMR	Q9SSX0. Positions 3-186.
ModBase	Search...
Genome annotation databases
GeneID	4325879.
KEGG	osa:4325879.
NMPDR	fig|39947.1.peg.5556.
Organism-specific databases
Gramene	Q9SSX0.
Family and domain databases
InterPro	IPR003578. GTPase_Rho. IPR013753. Ras. IPR001806. Ras_trnsfrmng. IPR005225. Small_GTP_bd. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00174. RHO. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
ProDom	Q9SSX0. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

RAC1_ORYSJ

Accession

Primary (citable) accession number: Q9SSX0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	May 1, 2000
Last modified:	July 22, 2008
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)