Reviewed, UniProtKB/Swiss-Prot P19171 (CHIB_ARATH)

Names and origin

Protein names

Recommended name:
    Basic endochitinase B
    EC=3.2.1.14
Alternative name(s):
    Pathogenesis-related protein 3
      Short name=PR-3
      Short name=AtChiB

Gene names

Name:	CHI-B
Synonyms:	PR3
Ordered Locus Names:	At3g12500
ORF Names:	T2E22.18, T2E22_119, MQC3.32

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Defense against chitin containing fungal pathogens. Seems particularly implicated in resistance to jasmonate-inducing pathogens such as A.brassicicola. In vitro antifungal activity against T.reesei, but not against A.solani, F.oxysporum, S.sclerotiorum, G.graminis and P.megasperma.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Subcellular location	Vacuole. Note= Vacuolar and protoplast.
Tissue specificity	High constitutive level in roots with lower levels in leaves and flowering shoots.
Induction	Ethylene induces high levels of systemic expression of basic chitinase with expression increasing with plant age. Locally and systemically induced by jasmonic acid (JA) and pathogens such as A.brassicicola and P.syringae, particularly in case of hypersensitive responses (HR). Not induced by wounding.
Sequence similarities	Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily. Contains 1 chitin-binding type-1 domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Hypersensitive response Plant defense Polysaccharide degradation
Cellular component	Vacuole
Domain	Signal
Ligand	Chitin-binding
Molecular function	Antimicrobial Fungicide Glycosidase Hydrolase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: InterPro defense response to fungus Inferred from electronic annotation. Source: UniProtKB-KW plant-type hypersensitive response Inferred from electronic annotation. Source: UniProtKB-KW xenobiotic metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	vacuole Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitin binding Inferred from electronic annotation. Source: InterPro chitinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 33	33
	Chain	34 – 328	295	Basic endochitinase B		PRO_0000005287
	Propeptide	329 – 335	7	Removed in mature form Probable		PRO_0000005288
Regions
	Domain	34 – 75	42	Chitin-binding type-1
Amino acid modifications
	Disulfide bond	36 ↔ 51		By similarity
	Disulfide bond	45 ↔ 57		By similarity
	Disulfide bond	50 ↔ 64		By similarity
	Disulfide bond	69 ↔ 73		By similarity
	Disulfide bond	107 ↔ 169		By similarity
	Disulfide bond	181 ↔ 189		By similarity
	Disulfide bond	288 ↔ 320		By similarity
Natural variations
	Natural variant	17	1	N → K in strain: cv. Bl-1, cv. Shokei and cv. Yo-0.
	Natural variant	106	1	A → T in strain: cv. Aa-0.
	Natural variant	127	1	G → S in strain: cv. Yo-0.
	Natural variant	206	1	N → D in strain: cv. Ci-0.

Sequences

	Sequence		Length	Mass (Da)
	P19171-1 [UniParc]. Last modified August 30, 2005. Version 3. Checksum: 0973CE89B30ABF61	FASTA	335	36,184
	10 20 30 40 50 60 MPPQKENHRT LNKMKTNLFL FLIFSLLLSL SSAEQCGRQA GGALCPNGLC CSEFGWCGNT 70 80 90 100 110 120 EPYCKQPGCQ SQCTPGGTPP GPTGDLSGII SSSQFDDMLK HRNDAACPAR GFYTYNAFIT 130 140 150 160 170 180 AAKSFPGFGT TGDTATRKKE VAAFFGQTSH ETTGGWATAP DGPYSWGYCF KQEQNPASDY 190 200 210 220 230 240 CEPSATWPCA SGKRYYGRGP MQLSWNYNYG LCGRAIGVDL LNNPDLVAND AVIAFKAAIW 250 260 270 280 290 300 FWMTAQPPKP SCHAVIAGQW QPSDADRAAG RLPGYGVITN IINGGLECGR GQDGRVADRI 310 320 330 GFYQRYCNIF GVNPGGNLDC YNQRSFVNGL LEAAI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of the genes encoding basic and acidic chitinase in Arabidopsis thaliana." Samac D.A., Hironaka C.M., Yallaly P.E., Shah D.M. Plant Physiol. 93:907-914(1990) [PubMed: 16667600] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, INDUCTION. Strain: cv. Columbia.
[2]	"DNA variation in the basic chitinase locus (ChiB) region of the wild plant Arabidopsis thaliana." Kawabe A., Miyashita N.T. Genetics 153:1445-1453(1999) [PubMed: 10545472] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS. Strain: cv. Aa-0, cv. Al-0, cv. Bl-1, cv. Bla-10, cv. Bs-1, cv. Chi-0, cv. Ci-0, cv. Es-0, cv. Gr-1, cv. Hiroshima, cv. Ita-0, cv. Kn-0, cv. Mt-0, cv. Pog-0, cv. Shokei and cv. Yo-0.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 9-335. Strain: cv. Columbia.
[6]	"Purification and characterization of an antifungal chitinase from Arabidopsis thaliana." Verburg J.G., Huynh Q.K. Plant Physiol. 95:450-455(1991) [PubMed: 16668004] [Abstract] Cited for: PROTEIN SEQUENCE OF 237-256, ANTIFUNGAL ACTIVITY.
[7]	"Analysis of ethylene signal-transduction kinetics associated with seedling-growth response and chitinase induction in wild-type and mutant Arabidopsis." Chen Q.G., Bleecker A.B. Plant Physiol. 108:597-607(1995) [PubMed: 7610160] [Abstract] Cited for: INDUCTION.
[8]	"Separate jasmonate-dependent and salicylate-dependent defense-response pathways in Arabidopsis are essential for resistance to distinct microbial pathogens." Thomma B.P.H.J., Eggermont K., Penninckx I.A.M.A., Mauch-Mani B., Vogelsang R., Cammue B.P.A., Broekaert W.F. Proc. Natl. Acad. Sci. U.S.A. 95:15107-15111(1998) [PubMed: 9844023] [Abstract] Cited for: FUNCTION, INDUCTION.
[9]	"Rhizobacteria-mediated induced systemic resistance (ISR) in Arabidopsis is not associated with a direct effect on expression of known defense-related genes but stimulates the expression of the jasmonate-inducible gene Atvsp upon challenge." van Wees S.C., Luijendijk M., Smoorenburg I., van Loon L.C., Pieterse C.M. Plant Mol. Biol. 41:537-549(1999) [PubMed: 10608663] [Abstract] Cited for: INDUCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M38240 Genomic DNA. Translation: AAA32769.1. AB023448 Genomic DNA. Translation: BAA82810.1. AB023449 Genomic DNA. Translation: BAA82811.1. AB023450 Genomic DNA. Translation: BAA82812.1. AB023451 Genomic DNA. Translation: BAA82813.1. AB023452 Genomic DNA. Translation: BAA82814.1. AB023453 Genomic DNA. Translation: BAA82815.1. AB023454 Genomic DNA. Translation: BAA82816.1. AB023455 Genomic DNA. Translation: BAA82817.1. AB023456 Genomic DNA. Translation: BAA82818.1. AB023457 Genomic DNA. Translation: BAA82819.1. AB023458 Genomic DNA. Translation: BAA82820.1. AB023459 Genomic DNA. Translation: BAA82821.1. AB023460 Genomic DNA. Translation: BAA82822.1. AB023461 Genomic DNA. Translation: BAA82823.1. AB023462 Genomic DNA. Translation: BAA82824.1. AB023463 Genomic DNA. Translation: BAA82825.1. AP002047 Genomic DNA. Translation: BAB03157.1. Different initiation. AC069474 Genomic DNA. Translation: AAG51023.1. AY054628 mRNA. Translation: AAK96819.1. Different initiation. AY081519 mRNA. Translation: AAM10081.1.
PIR	B45511.
UniGene	At.296
3D structure databases
HSSP	HSSP built from PDB template 2BAA based on UniProtKB P23951.
SMR	P19171. Positions 34-326.
ModBase	Search...
Genome annotation databases
GenomeReviews	Gene locus AT3G12500 in contig BA000014_GR.
NMPDR	fig|3702.1.peg.13324.
Organism-specific databases
TAIR	At3g12500.
Gene expression databases
ArrayExpress	P19171.
GermOnline	AT3G12500. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001002. Chitin_bd_1. IPR016283. Glyco_hydro_19. IPR000726. Glyco_hydro_19_cat. [Graphical view]
Gene3D	G3DSA:3.30.60.10. Chitin_bd_1. 1 hit.
PANTHER	PTHR22595. Glyco_hydro_19_cat. 1 hit.
Pfam	PF00187. Chitin_bind_1. 1 hit. PF00182. Glyco_hydro_19. 1 hit. [Graphical view]
PIRSF	PIRSF001060. Endochitinase. 1 hit.
PRINTS	PR00451. CHITINBINDNG.
ProDom	PD000609. Chitin_binding_1. 1 hit. PD354900. Glyco_hydro_19. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00270. ChtBD1. 1 hit. [Graphical view]
PROSITE	PS00026. CHIT_BIND_I_1. 1 hit. PS50941. CHIT_BIND_I_2. 1 hit. PS00773. CHITINASE_19_1. 1 hit. PS00774. CHITINASE_19_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHIB_ARATH

Accession

Primary (citable) accession number: P19171
Secondary accession number(s): Q9S7J5 , Q9S838, Q9SXJ2, Q9SXJ3, Q9SXJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	August 30, 2005
Last modified:	November 25, 2008
This is version 96 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)