Reviewed, UniProtKB/Swiss-Prot Q9TTC2 (GAG_KORV)

Names and origin

Protein names

Recommended name:
    Gag polyprotein
Alternative name(s):
    Core polyprotein
Cleaved into the following 4 chains:
    1- Recommended name:
            Matrix protein p15
                Short name=MA
    2- Recommended name:
            RNA-binding phosphoprotein p12
    3- Recommended name:
            Capsid protein p30
                Short name=CA
    4- Recommended name:
            Nucleocapsid protein p10
                Short name=NC

Gene names

Name:

gag

Organism

Koala retrovirus (KoRV)

Taxonomic identifier

394239 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Gammaretrovirus › unclassified Gammaretrovirus

Virus host

Phascolarctos cinereus (Koala) [TaxID: 38626]

Protein attributes

Sequence length	521 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane. Also mediates nuclear localization of the preintegration complex By similarity. Capsid protein p30 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex By similarity. Nucleocapsid protein p10 is involved in the packaging and encapsidation of two copies of the genome By similarity.
Subcellular location	Matrix protein p15: VirionPotential. Capsid protein p30: VirionPotential. Nucleocapsid protein p10: VirionPotential.
Domain	Late-budding domains (L domains) are short sequence motifs essential for viral particle release. They can occur individually or in close proximity within structural proteins. They interacts with sorting cellular proteins of the multivesicular body (MVB) pathway. Most of these proteins are class E vacuolar protein sorting factors belonging to ESCRT-I, ESCRT-II or ESCRT-III complexes. Inner coat protein p12 contains one L domain: a PPPY motif which probably binds to the WW domains of HECT (homologous to E6-AP C-terminus) E3 ubiquitin ligases By similarity.
Post-translational modification	Specific enzymatic cleavages by the viral protease yield mature proteins.
Miscellaneous	Koala retrovirus is an both a circulating virus and an endogenous retrovirus of koala, except in some isolated populations in south Australia.
Sequence similarities	Contains 1 CCHC-type zinc finger.

Ontologies

Keywords
Cellular component	Capsid protein Viral matrix protein Virion
Domain	Zinc-finger
Ligand	Metal-binding Viral nucleoprotein Zinc
PTM	Lipoprotein Myristate
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Initiator methionine	1	1	Removed; by host By similarity
	Chain	2 – 125	124	Matrix protein p15
	Chain	126 – 195	70	RNA-binding phosphoprotein p12
	Chain	196 – 454	259	Capsid protein p30
	Chain	455 – 521	67	Nucleocapsid protein p10
Regions
	Zinc finger	490 – 507	18	CCHC-type
	Motif	140 – 142	3	PPPY motif
Sites
	Site	196 – 197	2	Cleavage; by viral protease By similarity
	Site	455 – 456	2	Cleavage; by viral protease By similarity
Amino acid modifications
	Lipidation	2	1	N-myristoyl glycine; by host By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9TTC2-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: C4793022CDE3FCAF	FASTA	521	58,274
	10 20 30 40 50 60 MGQGESTPLS LTLDHWKDVK TRAHNLSVEI RKGKWQTFCS SEWPTFEVGW PPEGTFNPSI 70 80 90 100 110 120 ISAVKRIVFQ ETGGHPDQVP YIIVWQDLSN SPPPWVPPLA KIAVASGQDN GRKSAGGRPS 130 140 150 160 170 180 APSRLPIYPE TDSLFLLSEP PPYPTSPPPP PAPHAARPAP GLMAEGLGSE GPAAGTRSRR 190 200 210 220 230 240 PRSPTGDTGP DSTVALPLRA VGPPAEPNGL VPLQYWPFSS ADLYNWKSNH PSFSENPTGL 250 260 270 280 290 300 TGLLESLMFS HQPTWDDCQQ LLQVLFTTEE RERILLEARK NVLGVNGAPT QLENLINEAF 310 320 330 340 350 360 PLNRPQWDHN TAEGRERLLV YRRTLVAGLK GAARRPTNLA KVREVLQGPT EPPSVFLERL 370 380 390 400 410 420 MEAYRRYTPF DPSSEGQKAA VAMSFIGQSA PDIKKKLQRL EGLQDHSLQD LIKEAEKVYH 430 440 450 460 470 480 KRETEEEKQE REKKETEERE RRRDRRQEKN LTKILAAVVS EKGFRGRQAG NLSNRAMRAP 490 500 510 520 REGRPPLDKD QCAYCKERGH WARECPRKKN ARETNVLTLG D

References

[1]

"The nucleotide sequence of koala (Phascolarctos cinereus) retrovirus: a novel type C endogenous virus related to Gibbon ape leukemia virus." Hanger J.J., Bromham L.D., McKee J.J., O'Brien T.M., Robinson W.F. J. Virol. 74:4264-4272(2000) [PubMed: 10756041] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF151794 Genomic DNA. Translation: AAF15097.1.
3D structure databases
HSSP	HSSP built from PDB template 1A6B based on UniProtKB P03332.
ModBase	Search...
Family and domain databases
InterPro	IPR000840. G_retro_matrix_N. IPR003036. Gag_p30. IPR013084. Znf_CCH_retrovir. IPR001878. Znf_CCHC. [Graphical view]
Gene3D	G3DSA:4.10.60.10. Znf_CCH_retrovir. 1 hit.
Pfam	PF01140. Gag_MA. 1 hit. PF02093. Gag_p30. 1 hit. PF00098. zf-CCHC. 1 hit. [Graphical view]
SMART	SM00343. ZnF_C2HC. 1 hit. [Graphical view]
PROSITE	PS50158. ZF_CCHC. 1 hit. [Graphical view]
ProDom	Q9TTC2. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

GAG_KORV

Accession

Primary (citable) accession number: Q9TTC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	January 23, 2007
Last modified:	July 22, 2008
This is version 38 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)