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Reviewed, UniProtKB/Swiss-Prot Q9U7D5 (ACM3_CAEEL)

Last modified July 22, 2008. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Muscarinic acetylcholine receptor gar-3
Alternative name(s):
    G-protein-linked acetylcholine receptor 3
Gene names
Name: gar-3
ORF Names: Y40H4A.1
OrganismCaenorhabditis elegans [Complete proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

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Protein attributes

Sequence length611 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Developmental stage

Isoform a and isoform b are expressed at all developmental stages examined, isoform a is more abundant at embryonic and early larval stages and isoform b at first larval instar.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform b (identifier: Q9U7D5-1)

Also known as: GAR-3a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform a (identifier: Q9U7D5-2)

Also known as: GAR-3b;

The sequence of this isoform differs from the canonical sequence as follows:
     271-296: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 611611Muscarinic acetylcholine receptor gar-3

Regions

Topological domain1 – 6767Extracellular Potential
Transmembrane68 – 88211 Potential
Topological domain89 – 10113Cytoplasmic Potential
Transmembrane102 – 122212 Potential
Topological domain123 – 14018Extracellular Potential
Transmembrane141 – 161213 Potential
Topological domain162 – 18120Cytoplasmic Potential
Transmembrane182 – 202214 Potential
Topological domain203 – 22725Extracellular Potential
Transmembrane228 – 248215 Potential
Topological domain249 – 525277Cytoplasmic Potential
Transmembrane526 – 546216 Potential
Topological domain547 – 55711Extracellular Potential
Transmembrane558 – 578217 Potential
Topological domain579 – 61133Cytoplasmic Potential

Amino acid modifications

Glycosylation281N-linked (GlcNAc...)
Glycosylation331N-linked (GlcNAc...)
Disulfide bond138 ↔ 218 By similarity

Natural variations

Alternative sequence271 – 29626Missing in isoform a.

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Sequences

Sequence LengthMass (Da)Tools
Isoform b (GAR-3a) [UniParc].

Last modified November 23, 2004. Version 2.
Checksum: F1DD615020CA3180

FASTA61169,563
        10         20         30         40         50         60 
MQSSSLGNAD DPRFRQTHLF QMLVKVINTS AENATKTAIA TSSTSTPSFV DTYSTSSLLG 

        70         80         90        100        110        120 
EEGRMVMIVV IGAMFALVTS LGNLMVMVSF KIDKQLQTIS NYFLFSLAVA DIAIGVISIP 

       130        140        150        160        170        180 
MFTYYTAIQK WDLGYTMCQF WLCIDYLMSN ASVLNLLLIS FDRYFSVTRP LSYRPRRTTK 

       190        200        210        220        230        240 
KALTMIACTY IISLILWPPW IISWPYIEGK FTAEPGTCVV QFLQTNPYVT VGTAVAAFYL 

       250        260        270        280        290        300 
PVTIMCILYT RVYWETQKRQ KEFGKLQATQ TWASDVVDRP STQSFRNSKM WKKVKKFSRR 

       310        320        330        340        350        360 
SMKRDVSSTS IIKSSGSMRK KNNQDGYVED SVTPCTSSRN SKRKSWLRNC TGKSNSSSED 

       370        380        390        400        410        420 
SSEAVAMNLD DTSLSSSHFA LSGSRRRNIS PPCTPMPTNF EDEEQTDAGA SMRNGSARFR 

       430        440        450        460        470        480 
SRPSDTGKNN NSDTYTVLIE LNDEGSRPSV RLSSCEPYLD EPISTRNRSK SDCNSEIDER 

       490        500        510        520        530        540 
RHSLLNKQSP FKNGRILKNF SSQERKSEKE QRKNERKQES KAAKTLSAIL CAFIATWTPY 

       550        560        570        580        590        600 
NLIVCWEAFF PNTVPNVLWT FSYFLCYINS TINPLCYALC NARFRHTYMR ILRCKFKAER 

       610 
PTMNQGYVRR N

« Hide

Isoform a (GAR-3b) [UniParc].

Checksum: 0770C89B756F71DC
Show »

58566,470

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References

« Hide 'large scale' references
[1]"Cloning and functional characterization of a Caenorhabditis elegans muscarinic acetylcholine receptor."
Hwang J.M., Chang D.-J., Kim U.S., Lee Y.-S., Park Y.-S., Kaang B.-K., Cho N.J.
Recept. Channels 6:415-424(1999) [PubMed: 10635059] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION.
Strain: Bristol N2.
[2]"Alternative splicing of the muscarinic acetylcholine receptor GAR-3 in Caenorhabditis elegans."
Park Y.-S., Kim S., Shin Y., Choi B., Cho N.J.
Biochem. Biophys. Res. Commun. 308:961-965(2003) [PubMed: 12927813] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B), FUNCTION, DEVELOPMENTAL STAGE.
[3]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
[4]"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed: 17761667] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-28 AND ASN-33, MASS SPECTROMETRY.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AF139093 mRNA. Translation: AAD48771.1.
AY221631 mRNA. Translation: AAP97492.1.
AL034391, Z81486 Genomic DNA. Translation: CAA22301.2.
AL034391, Z81486 Genomic DNA. Translation: CAE47471.1.
Z81486, AL034391 Genomic DNA. Translation: CAC42272.1.
Z81486, AL034391 Genomic DNA. Translation: CAE47465.1.
PIRT20171.
T26789.
RefSeqNP_001024236.1.
UniGeneCel.19494

3D structure databases

ModBaseSearch...

Protein family/group databases

GPCRDBSearch...

Genome annotation databases

EnsemblY40H4A.1. Caenorhabditis elegans. [Contig view]
GeneID179962.

Organism-specific databases

WormBaseWBGene00001519. gar-3.
WormPepY40H4A.1a. CE27783. [WorfDB]
Y40H4A.1b. CE35800. [WorfDB]

Gene expression databases

ArrayExpressQ9U7D5.

Family and domain databases

InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_supfam.
IPR000995. Musac_rcpt.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProDomQ9U7D5.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

ProtoNetSearch...

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Entry information

Entry nameACM3_CAEEL
AccessionPrimary (citable) accession number: Q9U7D5
Secondary accession number(s): Q7JKV1, Q9XW31
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: November 23, 2004
Last modified: July 22, 2008
This is version 53 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectCaenorhabditis annotation project

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Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents