Reviewed, UniProtKB/Swiss-Prot Q9UKB5 (AJAP1_HUMAN)

Names and origin

Protein names

Recommended name:
    Adherens junction-associated protein 1
Alternative name(s):
    Membrane protein shrew-1

Gene names

Name:	AJAP1
Synonyms:	MOT8, SHREW1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plays a role in cell adhesion and cell migration.
Subunit structure	Forms a complex with CDH1 and CTNNB1; interacts directly with CTNNB1. Interacts with AP1M2 and BSG/CD147.
Subcellular location	Basolateral cell membrane; Single-pass type III membrane protein. Apical cell membrane; Single-pass type III membrane protein. Cell junction › adherens junction. Note= Mainly basolateral. Localization is mediated by AP1M2.
Tissue specificity	Expressed in uterus and pancreas (at protein level).
Post-translational modification	Thr-237 and Ser-239 may be phosphorylated; however as this position is probably extracellular, the vivo relevance is not proven.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell junction Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane
PTM	Phosphoprotein
Gene Ontology (GO)
Cellular component	apical plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell basolateral plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 411	411	Adherens junction-associated protein 1
Regions
	Topological domain	1 – 282	282	Extracellular Potential
	Transmembrane	283 – 303	21	Signal-anchor for type III membrane protein Potential
	Topological domain	304 – 411	108	Cytoplasmic Potential
	Region	303 – 411	109	Targeting signals
	Compositional bias	175 – 237	63	Thr-rich
Amino acid modifications
	Modified residue	237	1	Phosphothreonine
	Modified residue	239	1	Phosphoserine
Natural variations
	Natural variant	263	1	G → R: dbSNP rs242056.
Experimental info
	Mutagenesis	303	1	L → A: Mistargeting to the apical membrane
	Mutagenesis	350	1	Y → A: Mistargeting to the apical membrane
	Mutagenesis	368	1	Y → A: Mistargeting to the apical membrane
	Mutagenesis	380	1	Y → A: Mistargeting to the apical membrane
	Mutagenesis	396 – 397	2	LI → HV: Mistargeting to the apical membrane
	Mutagenesis	396	1	L → A: Mistargeting to the apical membrane

Sequences

	Sequence		Length	Mass (Da)
	Q9UKB5-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 42C1C5F56B9FC975	FASTA	411	44,536
	10 20 30 40 50 60 MWIQQLLGLS SMSIRWPGRP LGSHAWILIA MFQLAVDLPA CEALGPGPEF WLLPRSPPRP 70 80 90 100 110 120 PRLWSFRSGQ PARVPAPVWS PRPPRVERIH GQMQMPRARR AHRPRDQAAA LVPKAGLAKP 130 140 150 160 170 180 PAAAKSSPSL ASSSSSSSSA VAGGAPEQQA LLRRGKRHLQ GDGLSSFDSR GSRPTTETEF 190 200 210 220 230 240 IAWGPTGDEE ALESNTFPGV YGPTTVSILQ TRKTTVAATT TTTTTATPMT LQTKGFTESL 250 260 270 280 290 300 DPRRRIPGGV STTEPSTSPS NNGEVTQPPR ILGEASGLAV HQIITITVSL IMVIAALITT 310 320 330 340 350 360 LVLKNCCAQS GNTRRNSHQR KTNQQEESCQ NLTDFPSARV PSSLDIFTAY NETLQCSHEC 370 380 390 400 410 VRASVPVYTD ETLHSTTGEY KSTFNGNRPS SSDRHLIPVA FVSEKWFEIS C

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Novel membrane protein shrew-1 targets to cadherin-mediated junctions in polarized epithelial cells." Bharti S., Handrow-Metzmacher H., Zickenheiner S., Zeitvogel A., Baumann R., Starzinski-Powitz A. Mol. Biol. Cell 15:397-406(2004) [PubMed: 14595118] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION, TOPOLOGY, INTERACTION WITH CDH1 AND CTNNB1. Tissue: Brain.
[2]	"Identification of genes differentially expressed in HT1080 human fibrosarcoma cells selected for invasive capacity in vitro." Kudoh S., Chung I.M., Tsuiji M., Tsuji T., Irimura T. Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fibrosarcoma.
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Rhodes S. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 179-411.
[5]	"The SHREW1 gene, frequently deleted in oligodendrogliomas, functions to inhibit cell adhesion and migration." McDonald J.M., Dunlap S., Cogdell D., Dunmire V., Wei Q., Starzinski-Powitz A., Sawaya R., Bruner J., Fuller G.N., Aldape K., Zhang W. Cancer Biol. Ther. 5:300-304(2006) [PubMed: 16410724] [Abstract] Cited for: FUNCTION, VARIANT ARG-263.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-237 AND SER-239, MASS SPECTROMETRY. Tissue: Epithelium.
[7]	"Targeting of transmembrane protein shrew-1 to adherens junctions is controlled by cytoplasmic sorting motifs." Jakob V., Schreiner A., Tikkanen R., Starzinski-Powitz A. Mol. Biol. Cell 17:3397-3408(2006) [PubMed: 16707570] [Abstract] Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF LEU-303; TYR-350; TYR-368; TYR-380; LEU-396 AND 396-LEU--ILE-397, INTERACTION WITH AP1M2.
[8]	"junction protein shrew-1 influences cell invasion and interacts with invasion-promoting protein CD147." Schreiner A., Ruonala M., Jakob V., Suthaus J., Boles E., Wouters F., Starzinski-Powitz A. Mol. Biol. Cell 18:1272-1281(2007) [PubMed: 17267690] [Abstract] Cited for: FUNCTION, INTERACTION WITH BSG.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY282806 mRNA. Translation: AAP35025.1. AF175409 mRNA. Translation: AAD53278.1. AL391808, AL023586, Z98886 Genomic DNA. Translation: CAH72438.1. AL023586, AL391808, Z98886 Genomic DNA. Translation: CAI20566.1. Z98886, AL023586, AL391808 Genomic DNA. Translation: CAI22750.1. AL049672 mRNA. Translation: CAB41245.1. Different initiation. AL049673 mRNA. Translation: CAB41246.1. Different initiation.
RefSeq	NP_001035943.1. NP_061324.1.
UniGene	Hs.25924
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q9UKB5.
Proteomic databases
PeptideAtlas	Q9UKB5.
Genome annotation databases
Ensembl	ENSG00000196581. Homo sapiens. [Contig view]
GeneID	55966.
KEGG	hsa:55966.
Organism-specific databases
HGNC	HGNC:30801. AJAP1.
HPA	HPA012157.
MIM	610972. gene.
PharmGKB	PA142672629.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	Q9UKB5.
Gene expression databases
ArrayExpress	Q9UKB5.
CleanEx	HS_AJAP1.
Family and domain databases
ProDom	Q9UKB5. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

AJAP1_HUMAN

Accession

Primary (citable) accession number: Q9UKB5
Secondary accession number(s): Q9Y229

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
Last sequence update:	May 1, 2000
Last modified:	July 22, 2008
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)