Reviewed, UniProtKB/Swiss-Prot Q9VVR4 (BGBP2_DROME)

Names and origin

Protein names

Recommended name:
    Gram-negative bacteria-binding protein 2

Gene names

Name:	GNBP2
ORF Names:	CG4144

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the recognition of invading micro-organisms. Binds specifically to beta-1,3-glucan and activates the phenoloxidase cascade By similarity.
Subcellular location	SecretedBy similarity.
Developmental stage	Expressed at very low levels during embryonic development and at moderate levels in the larva, prepupal, pupal and adult stages.
Sequence similarities	Belongs to the insect beta-1,3-glucan binding protein family.

Ontologies

Keywords
Biological process	Immune response Innate immunity
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro regulation of innate immune response Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	extracellular region Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	bacterial binding Inferred from sequence or structural similarity. Source: UniProtKB hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform B (identifier: Q9VVR4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform A (identifier: Q9VVR4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: Missing.

Isoform C (identifier: Q9VVR4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-51: Missing.
     291-292: YL → CE
     293-461: Missing.

Isoform D (identifier: Q9VVR4-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-51: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 461	441	Gram-negative bacteria-binding protein 2		PRO_0000002818
Amino acid modifications
	Glycosylation	71	1	N-linked (GlcNAc...) Potential
	Glycosylation	170	1	N-linked (GlcNAc...) Potential
	Glycosylation	177	1	N-linked (GlcNAc...) Potential
	Glycosylation	364	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 51	51	Missing in isoform C and isoform D.		VSP_051600
	Alternative sequence	1 – 29	29	Missing in isoform A.		VSP_026674
	Alternative sequence	291 – 292	2	YL → CE in isoform C.		VSP_051601
	Alternative sequence	293 – 461	169	Missing in isoform C.		VSP_051602
	Natural variant	75	1	V → G in strain: Netherlands line N30.
	Natural variant	400	1	I → M in strain: Netherlands line N07 and Netherlands line N14.

Sequences

	Sequence		Length	Mass (Da)
	Isoform B [UniParc]. Last modified October 1, 2002. Version 3. Checksum: F62524B64CBE150D	FASTA	461	52,856
	10 20 30 40 50 60 MRWEFLPCLL LLISNNKIFG FKVPSINFEM LKDEGFEVSI PDEPGIQRVF YMFQIDDTCP 70 80 90 100 110 120 ALMDYITEAV NGSWVSKQKM SLQNNDKLQI SMLVQFNEEI FEKSETRVII NTRLLTTKDS 130 140 150 160 170 180 SSRGITFLTG EGECQAYLAP AQQAKRCKAA QTIVSNGRHT CQGELIFEDN FSEAQLNKTT 190 200 210 220 230 240 WKHDIRQRMY HVEEELVAFD DAARNCFVKE GELHIVPTIA TEVTDGSFKL GDRCTAVESP 250 260 270 280 290 300 EQECNIAHGI FYSIKPPVFS AQIHTRNSFS FKFGKIVVRA KLPKGDWLFP YLMLQPVSTY 310 320 330 340 350 360 AETHYAKQLR IAYARGNANL RTKQGDDISG NHLYGGGVVW HHGNAVQFLK DKISNSHYGD 370 380 390 400 410 420 DFHNYTMIWQ RDKITLMVDD EVYGELYDGL PFFNEKCFII FGVTVGGFLN FDDSLLAKDV 430 440 450 460 KPYKNREPRA ALSFWQHRDA WAPTWGRHSA MVIDYVRVYA E
	Isoform A [UniParc]. Checksum: D0ED6EA819F4E2C8		432	49,404
	10 20 30 40 50 60 MLKDEGFEVS IPDEPGIQRV FYMFQIDDTC PALMDYITEA VNGSWVSKQK MSLQNNDKLQ 70 80 90 100 110 120 ISMLVQFNEE IFEKSETRVI INTRLLTTKD SSSRGITFLT GEGECQAYLA PAQQAKRCKA 130 140 150 160 170 180 AQTIVSNGRH TCQGELIFED NFSEAQLNKT TWKHDIRQRM YHVEEELVAF DDAARNCFVK 190 200 210 220 230 240 EGELHIVPTI ATEVTDGSFK LGDRCTAVES PEQECNIAHG IFYSIKPPVF SAQIHTRNSF 250 260 270 280 290 300 SFKFGKIVVR AKLPKGDWLF PYLMLQPVST YAETHYAKQL RIAYARGNAN LRTKQGDDIS 310 320 330 340 350 360 GNHLYGGGVV WHHGNAVQFL KDKISNSHYG DDFHNYTMIW QRDKITLMVD DEVYGELYDG 370 380 390 400 410 420 LPFFNEKCFI IFGVTVGGFL NFDDSLLAKD VKPYKNREPR AALSFWQHRD AWAPTWGRHS 430 AMVIDYVRVY AE
	Isoform C [UniParc]. Checksum: B5F544D12C9C5567		241	27,313
	10 20 30 40 50 60 MFQIDDTCPA LMDYITEAVN GSWVSKQKMS LQNNDKLQIS MLVQFNEEIF EKSETRVIIN 70 80 90 100 110 120 TRLLTTKDSS SRGITFLTGE GECQAYLAPA QQAKRCKAAQ TIVSNGRHTC QGELIFEDNF 130 140 150 160 170 180 SEAQLNKTTW KHDIRQRMYH VEEELVAFDD AARNCFVKEG ELHIVPTIAT EVTDGSFKLG 190 200 210 220 230 240 DRCTAVESPE QECNIAHGIF YSIKPPVFSA QIHTRNSFSF KFGKIVVRAK LPKGDWLFPC E
	Isoform D [UniParc]. Checksum: B97E75D2348B65E3		410	46,852
	10 20 30 40 50 60 MFQIDDTCPA LMDYITEAVN GSWVSKQKMS LQNNDKLQIS MLVQFNEEIF EKSETRVIIN 70 80 90 100 110 120 TRLLTTKDSS SRGITFLTGE GECQAYLAPA QQAKRCKAAQ TIVSNGRHTC QGELIFEDNF 130 140 150 160 170 180 SEAQLNKTTW KHDIRQRMYH VEEELVAFDD AARNCFVKEG ELHIVPTIAT EVTDGSFKLG 190 200 210 220 230 240 DRCTAVESPE QECNIAHGIF YSIKPPVFSA QIHTRNSFSF KFGKIVVRAK LPKGDWLFPY 250 260 270 280 290 300 LMLQPVSTYA ETHYAKQLRI AYARGNANLR TKQGDDISGN HLYGGGVVWH HGNAVQFLKD 310 320 330 340 350 360 KISNSHYGDD FHNYTMIWQR DKITLMVDDE VYGELYDGLP FFNEKCFIIF GVTVGGFLNF 370 380 390 400 410 DDSLLAKDVK PYKNREPRAA LSFWQHRDAW APTWGRHSAM VIDYVRVYAE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gram-negative bacteria-binding protein, a pattern recognition receptor for lipopolysaccharide and beta-1,3-glucan that mediates the signaling for the induction of innate immune genes in Drosophila melanogaster cells." Kim Y.-S., Ryu J.-H., Han S.-J., Choi K.-H., Nam K.-B., Jang I.-H., Lemaitre B., Brey P.T., Lee W.-J. J. Biol. Chem. 275:32721-32727(2000) [PubMed: 10827089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), DEVELOPMENTAL STAGE.
[2]	"Contrasting evolutionary patterns in Drosophila immune receptors." Jiggins F.M., Kim K.W. J. Mol. Evol. 63:769-780(2006) [PubMed: 17103056] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Netherlands line N01, Netherlands line N02, Netherlands line N03, Netherlands line N06, Netherlands line N07, Netherlands line N14, Netherlands line N15, Netherlands line N16, Netherlands line N17, Netherlands line N22, Netherlands line N29 and Netherlands line N30.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C). Strain: Berkeley. Tissue: Head.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF228473 mRNA. Translation: AAF33850.1. AM050207 Genomic DNA. Translation: CAJ19007.1. AM050207 Genomic DNA. Translation: CAJ19008.1. AM050207 Genomic DNA. Translation: CAJ19009.1. AM050207 Genomic DNA. Translation: CAJ19010.1. AM050208 Genomic DNA. Translation: CAJ19011.1. AM050208 Genomic DNA. Translation: CAJ19012.1. AM050208 Genomic DNA. Translation: CAJ19013.1. AM050208 Genomic DNA. Translation: CAJ19014.1. AM050209 Genomic DNA. Translation: CAJ19015.1. AM050209 Genomic DNA. Translation: CAJ19016.1. AM050209 Genomic DNA. Translation: CAJ19017.1. AM050209 Genomic DNA. Translation: CAJ19018.1. AM050210 Genomic DNA. Translation: CAJ19019.1. AM050210 Genomic DNA. Translation: CAJ19020.1. AM050210 Genomic DNA. Translation: CAJ19021.1. AM050210 Genomic DNA. Translation: CAJ19022.1. AM050211 Genomic DNA. Translation: CAJ19023.1. AM050211 Genomic DNA. Translation: CAJ19024.1. AM050211 Genomic DNA. Translation: CAJ19025.1. AM050211 Genomic DNA. Translation: CAJ19026.1. AM050212 Genomic DNA. Translation: CAJ19027.1. AM050212 Genomic DNA. Translation: CAJ19028.1. AM050212 Genomic DNA. Translation: CAJ19029.1. AM050212 Genomic DNA. Translation: CAJ19030.1. AM050213 Genomic DNA. Translation: CAJ19031.1. AM050213 Genomic DNA. Translation: CAJ19032.1. AM050213 Genomic DNA. Translation: CAJ19033.1. AM050213 Genomic DNA. Translation: CAJ19034.1. AM050214 Genomic DNA. Translation: CAJ19035.1. AM050214 Genomic DNA. Translation: CAJ19036.1. AM050214 Genomic DNA. Translation: CAJ19037.1. AM050214 Genomic DNA. Translation: CAJ19038.1. AM050215 Genomic DNA. Translation: CAJ19039.1. AM050215 Genomic DNA. Translation: CAJ19040.1. AM050215 Genomic DNA. Translation: CAJ19041.1. AM050215 Genomic DNA. Translation: CAJ19042.1. AM050216 Genomic DNA. Translation: CAJ19043.1. AM050216 Genomic DNA. Translation: CAJ19044.1. AM050216 Genomic DNA. Translation: CAJ19045.1. AM050216 Genomic DNA. Translation: CAJ19046.1. AM050217 Genomic DNA. Translation: CAJ19047.1. AM050217 Genomic DNA. Translation: CAJ19048.1. AM050217 Genomic DNA. Translation: CAJ19049.1. AM050217 Genomic DNA. Translation: CAJ19050.1. AM050218 Genomic DNA. Translation: CAJ19051.1. AM050218 Genomic DNA. Translation: CAJ19052.1. AM050218 Genomic DNA. Translation: CAJ19053.1. AM050218 Genomic DNA. Translation: CAJ19054.1. AE014296 Genomic DNA. Translation: AAF49245.3. AE014296 Genomic DNA. Translation: AAF49246.3. AE014296 Genomic DNA. Translation: AAN11665.1. AE014296 Genomic DNA. Translation: AAN11666.1. AY058277 mRNA. Translation: AAL13506.1.
RefSeq	NP_524141.1. NP_730350.1. NP_730351.1. NP_730352.1.
UniGene	Dm.1608
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	CG4144. Drosophila melanogaster. [Contig view]
GeneID	40033.
KEGG	dme:Dmel_CG4144.
Organism-specific databases
FlyBase	FBgn0040322. GNBP2.
Phylogenomic databases
HOGENOM	Q9VVR4.
Gene expression databases
ArrayExpress	Q9VVR4.
GermOnline	CG4144. Drosophila melanogaster.
Family and domain databases
InterPro	IPR013320. ConA_like_subgrp. IPR000757. Glyco_hydro_16. [Graphical view]
Gene3D	G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.
Pfam	PF00722. Glyco_hydro_16. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	816648.

Entry information

Entry name

BGBP2_DROME

Accession

Primary (citable) accession number: Q9VVR4
Secondary accession number(s): A0ZWZ5 , A0ZWZ8, A0ZX11, A0ZX12, A0ZX13, A0ZX39, A0ZX40, A0ZX41, A0ZX42, Q8IQT6, Q8IQT7, Q95U68, Q9NHA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	October 1, 2002
Last modified:	November 25, 2008
This is version 45 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project