Reviewed, UniProtKB/Swiss-Prot Q9VVW1 (A76A_DROME)

Names and origin

Protein names

Recommended name:
    Accessory gland protein Acp76A

Gene names

Name:	Acp76A
ORF Names:	CG3801

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Responsible for physiological and behavioral changes in mated female flies. May play a role in accessory protein regulation and/or in the coagulation of seminal fluid to form a mating plug.
Subcellular location	SecretedProbable.
Tissue specificity	Main cells of accessory gland and seminal fluid.
Sequence similarities	Belongs to the serpin family.

Ontologies

Keywords
Biological process	Behavior
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 386	364	Accessory gland protein Acp76A
Sites
	Site	339 – 340	2	Reactive bond By similarity
Amino acid modifications
	Glycosylation	24	1	N-linked (GlcNAc...) Potential
	Glycosylation	378	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	22	1	Q → H in strain: ZIM5C, ZIM10C, ZIM16C, ZIM18C, ZIM28C, ZIM30C, ZIM34C and ZIM53C.
	Natural variant	22	1	Q → P in strain: ZIM7C and ZIM12C.
	Natural variant	332 – 337	6	EVVDDI → GKRNTR in strain: ZIM28C.
	Natural variant	338	1	D → N in strain: Canton-S and ZIM34C.
	Natural variant	359	1	I → V in strain: Canton-S, ZIM12C and ZIM16C.
	Natural variant	381	1	K → M in strain: ZIM5C and ZIM16C.
Experimental info
	Sequence conflict	7	1	I → T in AAB96393. Ref.1
	Sequence conflict	64 – 65	2	NN → TI in AAG38149 and AAG38150. Ref.4
	Sequence conflict	91	1	Y → S in AAG38149, AAG38150, AAG38151, AAG38152, AAG38153, AAG38154, AAG38155, AAG38156, AAG38157 and AAG38158. Ref.4
	Sequence conflict	141	1	K → T in AAG38149, AAG38150, AAG38151, AAG38152, AAG38153, AAG38154, AAG38155, AAG38156, AAG38157 and AAG38158. Ref.4
	Sequence conflict	145	1	E → A in AAG38149, AAG38150, AAG38151, AAG38152, AAG38153, AAG38154, AAG38155, AAG38156, AAG38157 and AAG38158. Ref.4
	Sequence conflict	165 – 167	3	NAG → YAA in AAG38149 and AAG38150. Ref.4
	Sequence conflict	172	1	A → S in AAG38149, AAG38150, AAG38151, AAG38152, AAG38153, AAG38154, AAG38155, AAG38156, AAG38157 and AAG38158. Ref.4
	Sequence conflict	182	1	W → C in AAG38149, AAG38150, AAG38151, AAG38152, AAG38153, AAG38154, AAG38155, AAG38156, AAG38157 and AAG38158. Ref.4
	Sequence conflict	191	1	N → I in AAG38149, AAG38150, AAG38151, AAG38152, AAG38153, AAG38154, AAG38155, AAG38156, AAG38157 and AAG38158. Ref.4
	Sequence conflict	202	1	Y → H in AAG38149, AAG38150, AAG38151, AAG38152, AAG38153, AAG38154, AAG38155, AAG38156, AAG38157 and AAG38158. Ref.4
	Sequence conflict	238	1	N → Y in AAG38149, AAG38150, AAG38151, AAG38152, AAG38153, AAG38154, AAG38155, AAG38156, AAG38157 and AAG38158. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9VVW1-1 [UniParc]. Last modified March 1, 2001. Version 2. Checksum: D2640A616B3F0FC8	FASTA	386	43,967
	10 20 30 40 50 60 MGNHQVIFLV LCTSLLFQNT IQQNVSFQLI REIDRYTPEN FVLSVLNIEM ILFEIHAAKA 70 80 90 100 110 120 VESNNDLERS LIINFGYSEA RQEVLDWGLR YKKASSAKFQ MANKVAVSQK LPLSQKLRLV 130 140 150 160 170 180 NEVLMTSAKK YDVTKDVRPS KLMDEWLSSH LDGVLANFVQ EKKLNAGENI VAISGMTVTP 190 200 210 220 230 240 LWASHFQSEI NRYFVNNPGT GYASKDPTCV PMMHSLSSFE TMSTDEAKGI YIPFSSANLG 250 260 270 280 290 300 MLILLPRKGV TCKDILDNLN NQINVEYNDH KDVHLLLPIF KEKFDYNIAK FFNGINIEDT 310 320 330 340 350 360 FKDSAFKSKA KIKINNFRVN HGIRFQPILR LEVVDDIDTG KTETFEVNRP FVFVIKDKIN 370 380 VYAVGRIENL DGLTDKVNCS KKYADL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"New genes for male accessory gland proteins in Drosophila melanogaster." Wolfner M.F., Harada H.A., Bertram M.J., Stelick T.J., Kraus K.W., Kalb J.M., Lung Y.O., Neubaum D.M., Park M., Tram U.K. Insect Biochem. Mol. Biol. 27:825-834(1997) [PubMed: 9474779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY. Strain: Canton-S. Tissue: Male accessory gland.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	"Molecular population genetics of male accessory gland proteins in Drosophila." Begun D.J., Whitley P., Todd B.L., Waldrip-Dail H.M., Clark A.G. Genetics 156:1879-1888(2000) [PubMed: 11102381] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 22-386. Strain: ZIM10C, ZIM12C, ZIM16C, ZIM18C, ZIM28C, ZIM30C, ZIM34C, ZIM53C, ZIM5C and ZIM7C.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U90947 Genomic DNA. Translation: AAB96393.1. AE014296 Genomic DNA. Translation: AAF49196.1. AY010627 Genomic DNA. Translation: AAG38149.1. AY010628 Genomic DNA. Translation: AAG38150.1. AY010629 Genomic DNA. Translation: AAG38151.1. AY010630 Genomic DNA. Translation: AAG38152.1. AY010631 Genomic DNA. Translation: AAG38153.1. AY010632 Genomic DNA. Translation: AAG38154.1. AY010633 Genomic DNA. Translation: AAG38155.1. AY010634 Genomic DNA. Translation: AAG38156.1. AY010635 Genomic DNA. Translation: AAG38157.1. AY010636 Genomic DNA. Translation: AAG38158.1.
RefSeq	NP_524153.1.
UniGene	Dm.27174
3D structure databases
HSSP	HSSP built from PDB template 1UHG based on UniProtKB P01012.
ModBase	Search...
Genome annotation databases
Ensembl	CG3801. Drosophila melanogaster. [Contig view]
GeneID	40078.
KEGG	dme:Dmel_CG3801.
Organism-specific databases
FlyBase	FBgn0015586. Acp76A.
Phylogenomic databases
HOGENOM	Q9VVW1.
Gene expression databases
ArrayExpress	Q9VVW1.
GermOnline	CG3801. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000215. Protease_inhib_I4_serpin. [Graphical view]
PANTHER	PTHR11461. Prot_inh_serpin. 1 hit.
SMART	SM00093. SERPIN. 1 hit. [Graphical view]
PROSITE	PS00284. SERPIN. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

A76A_DROME

Accession

Primary (citable) accession number: Q9VVW1
Secondary accession number(s): O46224

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	March 1, 2001
Last modified:	July 22, 2008
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project