Reviewed, UniProtKB/Swiss-Prot Q9WVL4 (RK_MOUSE)

Names and origin

Protein names

Recommended name:
    Rhodopsin kinase
      Short name=RK
    EC=2.7.11.14
Alternative name(s):
    G protein-coupled receptor kinase 1

Gene names

Name:	Grk1
Synonyms:	Rhok

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	564 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Phosphorylates rhodopsin thereby initiating its deactivation.
Catalytic activity	ATP + [rhodopsin] = ADP + [rhodopsin] phosphate.
Subcellular location	Membrane; Lipid-anchor.
Post-translational modification	Autophosphorylated By similarity. Farnesylation is required for full activity By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. Contains 1 RGS domain.

Ontologies

Keywords
Cellular component	Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Lipoprotein Methylation Phosphoprotein Prenylation
Gene Ontology (GO)
Biological process	apoptosis Ref.1 Inferred from mutant phenotype. Source: MGI photoreceptor cell morphogenesis Ref.1 Inferred from mutant phenotype. Source: MGI positive regulation of phosphorylation Ref.1 Inferred from mutant phenotype. Source: MGI post-embryonic retina morphogenesis in camera-type eye Ref.1 Inferred from mutant phenotype. Source: MGI response to light stimulus Ref.1 Inferred from mutant phenotype. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 561	561	Rhodopsin kinase
	Propeptide	562 – 564	3	Removed in mature form By similarity
Regions
	Domain	58 – 175	118	RGS
	Domain	190 – 455	266	Protein kinase
	Domain	456 – 521	66	AGC-kinase C-terminal
	Nucleotide binding	196 – 204	9	ATP By similarity
	Region	1 – 189	189	N-terminal
	Region	456 – 564	109	C-terminal
Sites
	Active site	317	1	Proton acceptor By similarity
	Binding site	219	1	ATP By similarity
Amino acid modifications
	Modified residue	21	1	Phosphoserine; by autocatalysis By similarity
	Modified residue	491	1	Phosphoserine; by autocatalysis By similarity
	Modified residue	492	1	Phosphothreonine; by autocatalysis By similarity
	Modified residue	561	1	Cysteine methyl ester By similarity
	Lipidation	561	1	S-farnesyl cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9WVL4-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: EF0795FAE42BA5DB	FASTA	564	63,837
	10 20 30 40 50 60 MDFGSLETVV ANSAFIAARG SFDGSSTPSS RDKKYLAKLR LPPLSKCEGL RDNLSLEFES 70 80 90 100 110 120 LCSEQPIGKR LFQQFLKTDE RHVPALELWK DIEDYDTADD DLRPQKAQAI LAEYLDPQGT 130 140 150 160 170 180 LFCNFLDQGM VTRVKEGPTG SQDGLFQPLL QATMEHLSQA PFQEYLESLY FLRFLQWKWL 190 200 210 220 230 240 EAQPIGEDWF LDFRVLGKGG FGEVSACQMK ATGKMYACKK LNKKRLKKKK GYQGAIVEKR 250 260 270 280 290 300 ILTKVHSRFI VSLAYAFETK TDLCLVMTIM NGGDVRYHIY NVDEDNPGFS EPRAIYYTAQ 310 320 330 340 350 360 IISGLEHLHQ RRIVYRDLKP ENVLLDNDGN IRISDLGLAV ELKEGQNKTK GYAGTPGFMA 370 380 390 400 410 420 PELLRGEEYD FSVDYFALGV TLYEMIAARG PFRARGEKVE NKELKQRIIS EPVKYPEKFS 430 440 450 460 470 480 QASKDFCEQL LEKDPEKRLG FRDGTCDALR ANVLFKDISW RQLEAGMLIP PFIPDSRTVY 490 500 510 520 530 540 AKNIQDVGAF STVKGVVFDK ADTEFFQEFA SGNCSIPWQE EMIETGLFGD LNVWRADGQM 550 560 PDDMKGITTE EAAPTAKSGM CLIS

References

[1]	"Abnormal photoresponses and light-induced apoptosis in rods lacking rhodopsin kinase." Chen C.K., Burns M.E., Spencer M., Niemi G.A., Chen J., Hurley J.B., Baylor D.A., Simon M.I. Proc. Natl. Acad. Sci. U.S.A. 96:3718-3722(1999) [PubMed: 10097103] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF085240 mRNA. Translation: AAD40189.1.
UniGene	Mm.257501
3D structure databases
HSSP	HSSP built from PDB template 1GZK based on UniProtKB P31751.
ModBase	Search...
PTM databases
PhosphoSite	Q9WVL4.
Genome annotation databases
Ensembl	ENSMUSG00000031450. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:1345146. Grk1.
Phylogenomic databases
HOGENOM	Q9WVL4.
HOVERGEN	Q9WVL4.
Gene expression databases
ArrayExpress	Q9WVL4.
CleanEx	MM_GRK1.
GermOnline	ENSMUSG00000031450. Mus musculus.
Family and domain databases
InterPro	IPR000239. GPCR_kinase. IPR000961. Pkinase_C. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR000342. Regulat_G_prot_signal. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. PF00615. RGS. 1 hit. [Graphical view]
PRINTS	PR00717. GPCRKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00315. RGS. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS50132. RGS. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

RK_MOUSE

Accession

Primary (citable) accession number: Q9WVL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	November 1, 1999
Last modified:	July 22, 2008
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)