Reviewed, UniProtKB/Swiss-Prot Q9Y2D5 (AKAP2_HUMAN)

Names and origin

Protein names

Recommended name:
    A-kinase anchor protein 2
      Short name=AKAP-2
Alternative name(s):
    Protein kinase A-anchoring protein 2
      Short name=PRKA2
    AKAP-KL

Gene names

Name:	AKAP2
Synonyms:	KIAA0920

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	872 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds to regulatory subunit (RII) of protein kinase A. May be involved in establishing polarity in signaling systems or in integrating PKA-RII isoforms with downstream effectors to capture, amplify and focus diffuse, trans-cellular signals carried by cAMP By similarity.
Miscellaneous	Can be differentially expressed as a natural AKAP2-PALM2 chimeric protein.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
PTM	Phosphoprotein
Gene Ontology (GO)
Molecular function	enzyme binding Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q9Y2D5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: Q9Y2D5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     828-840: Missing.

Notes: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 872	872	A-kinase anchor protein 2
Regions
	Region	566 – 579	14	PKA-RII subunit binding domain
	Coiled coil	213 – 290	78	Potential
	Coiled coil	710 – 748	39	Potential
	Compositional bias	274 – 294	21	Gln-rich
Amino acid modifications
	Modified residue	152	1	Phosphoserine
	Modified residue	393	1	Phosphoserine
	Modified residue	720	1	Phosphoserine
Natural variations
	Alternative sequence	828 – 840	13	Missing in isoform B.
	Natural variant	561	1	L → S: dbSNP rs914358.
Experimental info
	Sequence conflict	282	1	K → Q in AK057098. Ref.3
	Sequence conflict	647	1	E → G in CAB53707. Ref.4
	Sequence conflict	651	1	L → P in AK057098. Ref.3
	Sequence conflict	719	1	L → S in AK057098. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified January 9, 2007. Version 2. Checksum: E962F82413EC4D7E	FASTA	872	96,101
	10 20 30 40 50 60 MEIEVSVAEC KSVPGITSTP HPMDHPSAFY SPPHNGLLTD HHESLDNDVA REIRYLDEVL 70 80 90 100 110 120 EANCCDSAVD GTYNGTSSPE PGAVVLVGGL SPPVHEATQP EPTERTASRQ APPHIELSNS 130 140 150 160 170 180 SPDPMAEAER TNGHSPSQPR DALGDSLQVP VSPSSTTSSR CSSRDGEFTL TTLKKEAKFE 190 200 210 220 230 240 LRAFHEDKKP SKLFEDDEHE KEQYCIRKVR PSEEMLELEK ERRELIRSQA VKKNPGIAAK 250 260 270 280 290 300 WWNPPQEKTI EEQLDEEHLE SHKKYKERKE RRAQQEQLLL QKQLQQQQQQ PPSQLCTAPA 310 320 330 340 350 360 SSHERASMID KAKEDIVTEQ IDFSAARKQF QLMENSRQAV AKGQSTPRLF SIKPFYRPLG 370 380 390 400 410 420 SVNSDKPLTN PRPPSVGGPP EDSGASAAKG QKSPGALETP SAAGSQGNTA SQGKEGPYSE 430 440 450 460 470 480 PSKRGPLSKL WAEDGEFTSA RAVLTVVKDD DHGILDQFSR SVNVSLTQEE LDSGLDELSV 490 500 510 520 530 540 RSQDTTVLET LSNDFSMDNI SDSGASNETT NALQENSLAD FSLPQTPQTD NPSEGRGEGV 550 560 570 580 590 600 SKSFSDHGFY SPSSTLGDSP LVDDPLEYQA GLLVQNAIQQ AIAEQVDKAV SKTSRDGAEQ 610 620 630 640 650 660 QGPEATVEEA EAAAFGSEKP QSMFEPPQVS SPVQEKRDVL PKILPAEDRA LRERGPPQPL 670 680 690 700 710 720 PAVQPSGPIN MEETRPEGSY FSKYSEAAEL RSTASLLATQ ESDVMVGPFK LRSRKQRTLS 730 740 750 760 770 780 MIEEEIRAAQ EREEELKRQR QVLQSTQSPR TKNAPSLPSR TCYKTAPGKI EKVKPPPSPT 790 800 810 820 830 840 TEGPSLQPDL APEEAAGTQR PKNLMQTLME DYETHKSKRR ERMDDSSYTS KLLSCKVTSE 850 860 870 VLEATRVNRR KSALALRWEA GIYANQEEED NE
	Isoform B [UniParc]. Checksum: 6DCE563596DFBA4B		859	94,661
	10 20 30 40 50 60 MEIEVSVAEC KSVPGITSTP HPMDHPSAFY SPPHNGLLTD HHESLDNDVA REIRYLDEVL 70 80 90 100 110 120 EANCCDSAVD GTYNGTSSPE PGAVVLVGGL SPPVHEATQP EPTERTASRQ APPHIELSNS 130 140 150 160 170 180 SPDPMAEAER TNGHSPSQPR DALGDSLQVP VSPSSTTSSR CSSRDGEFTL TTLKKEAKFE 190 200 210 220 230 240 LRAFHEDKKP SKLFEDDEHE KEQYCIRKVR PSEEMLELEK ERRELIRSQA VKKNPGIAAK 250 260 270 280 290 300 WWNPPQEKTI EEQLDEEHLE SHKKYKERKE RRAQQEQLLL QKQLQQQQQQ PPSQLCTAPA 310 320 330 340 350 360 SSHERASMID KAKEDIVTEQ IDFSAARKQF QLMENSRQAV AKGQSTPRLF SIKPFYRPLG 370 380 390 400 410 420 SVNSDKPLTN PRPPSVGGPP EDSGASAAKG QKSPGALETP SAAGSQGNTA SQGKEGPYSE 430 440 450 460 470 480 PSKRGPLSKL WAEDGEFTSA RAVLTVVKDD DHGILDQFSR SVNVSLTQEE LDSGLDELSV 490 500 510 520 530 540 RSQDTTVLET LSNDFSMDNI SDSGASNETT NALQENSLAD FSLPQTPQTD NPSEGRGEGV 550 560 570 580 590 600 SKSFSDHGFY SPSSTLGDSP LVDDPLEYQA GLLVQNAIQQ AIAEQVDKAV SKTSRDGAEQ 610 620 630 640 650 660 QGPEATVEEA EAAAFGSEKP QSMFEPPQVS SPVQEKRDVL PKILPAEDRA LRERGPPQPL 670 680 690 700 710 720 PAVQPSGPIN MEETRPEGSY FSKYSEAAEL RSTASLLATQ ESDVMVGPFK LRSRKQRTLS 730 740 750 760 770 780 MIEEEIRAAQ EREEELKRQR QVLQSTQSPR TKNAPSLPSR TCYKTAPGKI EKVKPPPSPT 790 800 810 820 830 840 TEGPSLQPDL APEEAAGTQR PKNLMQTLME DYETHKSKRR ERMDDSSVLE ATRVNRRKSA 850 LALRWEAGIY ANQEEEDNE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Homo sapiens AKAP-2 complete cDNA sequence." Schuetze N., Reichel S., Ruecker N., Lechner A., Eulert J., Jakob F. Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
[2]	"Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 6:63-70(1999) [PubMed: 10231032] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Tissue: Brain.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B), VARIANT SER-561. Tissue: Small intestine.
[4]	The German cDNA consortium Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 539-872 (ISOFORM B). Tissue: Brain.
[5]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-152 AND SER-720, MASS SPECTROMETRY. Tissue: Epithelium.
[6]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-393 AND SER-720, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ303079 mRNA. Translation: CAC38839.1. Different initiation. AB023137 mRNA. Translation: BAA76764.2. Different initiation. AK057098 mRNA. No translation available. AL110268 mRNA. Translation: CAB53707.1.
PIR	T14787.
RefSeq	NP_009134.1. NP_671492.1.
UniGene	Hs.591908
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q9Y2D5.
Genome annotation databases
Ensembl	ENSG00000157654. Homo sapiens. [Contig view]
GeneID	445815.
KEGG	hsa:445815.
Organism-specific databases
HGNC	HGNC:372. AKAP2.
MIM	604582. gene.
PharmGKB	PA24666.
HUGE	Search...
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	Q9Y2D5.
Gene expression databases
ArrayExpress	Q9Y2D5.
GermOnline	ENSG00000157654. Homo sapiens.
Family and domain databases
ProDom	Q9Y2D5. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

AKAP2_HUMAN

Accession

Primary (citable) accession number: Q9Y2D5
Secondary accession number(s): Q9UG26

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	January 9, 2007
Last modified:	July 22, 2008
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)