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Reviewed, UniProtKB/Swiss-Prot Q9Y3Q7 (ADA18_HUMAN)

Last modified July 22, 2008. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    ADAM 18
Alternative name(s):
    A disintegrin and metalloproteinase domain 18
    Transmembrane metalloproteinase-like, disintegrin-like, and cysteine-rich protein III
      Short name=tMDC III
Gene names
Name: ADAM18
Synonyms: TMDC3
ORF Names: UNQ858/PRO1867
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length739 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Sperm surface membrane protein that may be involved in spermatogenesis and fertilization. This is a non catalytic metalloprotease-like protein By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Expressed specifically in testis.

Domain

A tripeptide motif (ECD) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding By similarity.

Post-translational modification

The prodomain and the metalloprotease-like domain are cleaved during the epididymal maturation of the spermatozoa By similarity.

Sequence similarities

Contains 1 disintegrin domain.

Contains 1 EGF-like domain.

Contains 1 peptidase M12B domain.

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Ontologies

Keywords

   Biological processDifferentiation
Spermatogenesis
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainEGF-like domain
Signal
Transmembrane
   Molecular functionDevelopmental protein
   PTMGlycoprotein

Gene Ontology (GO)

   Biological processspermatogenesis

Traceable author statement. Source: ProtInc

   Cellular componentmembrane fraction

Traceable author statement. Source: ProtInc

   Molecular functionmetallopeptidase activity

Traceable author statement. Source: ProtInc

Complete GO annotation...

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9Y3Q7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9Y3Q7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     197-220: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Signal peptide1 – 1616 Potential
Propeptide17 – 184168 Potential
Chain185 – 739555ADAM 18

Regions

Topological domain177 – 687511Extracellular Potential
Transmembrane688 – 70821 Potential
Topological domain709 – 73931Cytoplasmic Potential
Domain184 – 381198Peptidase M12B
Domain390 – 47990Disintegrin
Domain620 – 65435EGF-like
Compositional bias480 – 622143Cys-rich

Amino acid modifications

Glycosylation361N-linked (GlcNAc...) Potential
Glycosylation761N-linked (GlcNAc...) Potential
Glycosylation1221N-linked (GlcNAc...) Potential
Glycosylation1491N-linked (GlcNAc...) Potential
Glycosylation1561N-linked (GlcNAc...) Potential
Glycosylation1771N-linked (GlcNAc...) Potential
Glycosylation2941N-linked (GlcNAc...) Potential
Glycosylation3591N-linked (GlcNAc...) Potential
Glycosylation4651N-linked (GlcNAc...) Potential
Glycosylation5611N-linked (GlcNAc...) Potential
Glycosylation6111N-linked (GlcNAc...) Potential
Glycosylation6251N-linked (GlcNAc...) Potential
Disulfide bond293 ↔ 376 By similarity
Disulfide bond335 ↔ 360 By similarity
Disulfide bond337 ↔ 342 By similarity
Disulfide bond450 ↔ 471 By similarity
Disulfide bond624 ↔ 636 By similarity
Disulfide bond630 ↔ 642 By similarity
Disulfide bond644 ↔ 653 By similarity

Natural variations

Alternative sequence197 – 22024Missing in isoform 2.

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 2D8BE9A975072CDD

FASTA73982,856
        10         20         30         40         50         60 
MFLLLALLTE LGRLQAHEGS EGIFLHVTVP RKIKSNDSEV SERKMIYIIT IDGQPYTLHL 

        70         80         90        100        110        120 
GKQSFLPQNF LVYTYNETGS LHSVSPYFMM HCHYQGYAAE FPNSFVTLSI CSGLRGFLQF 

       130        140        150        160        170        180 
ENISYGIEPV ESSARFEHII YQMKNNDPNV SILAVNYSHI WQKDQPYKVP LNSQIKNLSK 

       190        200        210        220        230        240 
LLPQYLEIYI IVEKALYDYM GSEMMAVTQK IVQVIGLVNT MFTQFKLTVI LSSLELWSNE 

       250        260        270        280        290        300 
NQISTSGDAD DILQRFLAWK RDYLILRPHD IAYLLVYRKH PKYVGATFPG TVCNKSYDAG 

       310        320        330        340        350        360 
IAMYPDAIGL EGFSVIIAQL LGLNVGLTYD DITQCFCLRA TCIMNHEAVS ASGRKIFSNC 

       370        380        390        400        410        420 
SMHDYRYFVS KFETKCLQKL SNLQPLHQNQ PVCGNGILES NEECDCGNKN ECQFKKCCDY 

       430        440        450        460        470        480 
NTCKLKGSVK CGSGPCCTSK CELSIAGTPC RKSIDPECDF TEYCNGTSSN CVPDTYALNG 

       490        500        510        520        530        540 
RLCKLGTAYC YNGQCQTTDN QCAKIFGKGA QGAPFACFKE VNSLHERSEN CGFKNSQPLP 

       550        560        570        580        590        600 
CERKDVLCGK LACVQPHKNA NKSDAQSTVY SYIQDHVCVS IATGSSMRSD GTDNAYVADG 

       610        620        630        640        650        660 
TMCGPEMYCV NKTCRKVHLM GYNCNATTKC KGKGICNNFG NCQCFPGHRP PDCKFQFGSP 

       670        680        690        700        710        720 
GGSIDDGNFQ KSGDFYTEKG YNTHWNNWFI LSFCIFLPFF IVFTTVIFKR NEISKSCNRE 

       730 
NAEYNRNSSV VSESDDVGH

« Hide

Isoform 2 [UniParc].

Checksum: EF8EB71EBA6AB9DB
Show »

71580,183

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References

« Hide 'large scale' references
[1]"Nucleotide sequence of the human tMDC III sperm surface protein transcript."
Hall L., Frayne J., Dimsey E.A.
Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Testis.
[2]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
+Additional computationally mapped references.

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Cross-references

Sequence databases

AJ133004 mRNA. Translation: CAB40812.1.
AY358321 mRNA. Translation: AAQ88687.1.
BC121045 mRNA. Translation: AAI21046.1.
RefSeqNP_055052.1.
UniGeneHs.127930

3D structure databases

HSSPHSSP built from PDB template 1MPZ based on UniProtKB P83469.
ModBaseSearch...

Protein family/group databases

MEROPSM12.957.

PTM databases

PhosphoSiteQ9Y3Q7.

Genome annotation databases

EnsemblENSG00000168619. Homo sapiens. [Contig view]
GeneID8749.
KEGGhsa:8749.

Organism-specific databases

H-InvDBHIX0034291.
HGNCHGNC:196. ADAM18.
PharmGKBPA24513.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOGENOMQ9Y3Q7.
HOVERGENQ9Y3Q7.

Gene expression databases

ArrayExpressQ9Y3Q7.
CleanExHS_ADAM18.
GermOnlineENSG00000168619. Homo sapiens.

Family and domain databases

InterProIPR006586. ADAM_cysteine.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR000742. EGF_3.
IPR013111. EGF_extracell.
IPR013032. EGF_like_reg_CS.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
Gene3DG3DSA:4.10.70.10. Blood-coag_inhib_Disintegrin. 1 hit.
PfamPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF07974. EGF_2. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
ProDomPD000664. Disintegrin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
PROSITEPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00022. EGF_1. False negative.
PS01186. EGF_2. False negative.
PS50026. EGF_3. False negative.
[Graphical view]
BLOCKSSearch...

Other Resources

LinkHubQ9Y3Q7.
ProtoNetSearch...

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Entry information

Entry nameADA18_HUMAN
AccessionPrimary (citable) accession number: Q9Y3Q7
Secondary accession number(s): Q0VAI4, Q6UXJ9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: November 1, 1999
Last modified: July 22, 2008
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM

Peptidase families

Classification of peptidase families and list of entries

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents