Protein ariadne-1 homolog - Homo sapiens (Human)

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Reviewed, UniProtKB/Swiss-Prot Q9Y4X5 (ARI1_HUMAN)

Last modified July 22, 2008. Version 76. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Protein ariadne-1 homolog
      Short name(s)=ARI-1
Alternative name(s):
    Ubiquitin-conjugating enzyme E2-binding protein 1
    UbcH7-binding protein
    UbcM4-interacting protein
    HHARI
    H7-AP2
    Monocyte protein 6
      Short name(s)=MOP-6

Gene names

Name:	ARIH1
Synonyms:	ARI, MOP6, UBCH7BP
ORF Names:	HUSSY-27

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Might act as an E3 ubiquitin-protein ligase, or as part of the E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates.
Subunit structure	Interacts with UBE2L3.
Subcellular location	Cytoplasm. Note= Mainly cytoplasmic.
Tissue specificity	Widely expressed.
Sequence similarities	Contains 1 IBR-type zinc finger. Contains 2 RING-type zinc fingers.

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Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm
Domain	Coiled coil Repeat Zinc-finger
Ligand	Metal-binding Zinc
Technical term	3D-structure
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Ref.1 Traceable author statement. Source: UniProtKB
Cellular component	ubiquitin ligase complex Ref.1 Traceable author statement. Source: UniProtKB
Molecular function	protein binding Ref.1 Inferred from physical interaction. Source: UniProtKB small conjugating protein ligase activity Ref.1 Traceable author statement. Source: UniProtKB
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Chain

1 – 557

557

Protein ariadne-1 homolog

Regions

Zinc finger

186 – 235

RING-type 1

Zinc finger

256 – 317

IBR-type

Zinc finger

344 – 389

RING-type 2

Region

186 – 254

Interaction with UBE2L3

Coiled coil

433 – 449

Potential

Compositional bias

10 – 38

Asp/Glu-rich (acidic)

Compositional bias

51 – 92

Gly-rich

Experimental info

Mutagenesis

187 – 188

QI → HV: No loss of interaction with UBE2L3

Mutagenesis

188

I → A: Loss of interaction with UBE2L3

Mutagenesis

208

C → A or H: Loss of interaction with UBE2L3

Mutagenesis

258

Y → A: No loss of interaction with UBE2L3

Sequence conflict

122

E → D in AAD28088. Ref.2

Sequence conflict

237

D → N in CAA10274. Ref.3

Sequence conflict

303

F → S in CAA08817. Ref.5

Sequence conflict

309 – 316

ENWHDPVK → AIGMILFQ in CAA08817. Ref.5

Sequence conflict

322

K → T in CAA08817. Ref.5

Secondary structure

557

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

Q9Y4X5-1 [UniParc].

Last modified May 10, 2002. Version 2.
Checksum: DFFF8965DAB41DC8
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FASTA

557

64,118

        10         20         30         40         50         60 
MDSDEGYNYE FDEDEECSEE DSGAEEEEDE DDDEPDDDTL DLGEVELVEP GLGVGGERDG 

        70         80         90        100        110        120 
LLCGETGGGG GSALGPGGGG GGGGGGGGGG PGHEQEEDYR YEVLTAEQIL QHMVECIREV 

       130        140        150        160        170        180 
NEVIQNPATI TRILLSHFNW DKEKLMERYF DGNLEKLFAE CHVINPSKKS RTRQMNTRSS 

       190        200        210        220        230        240 
AQDMPCQICY LNYPNSYFTG LECGHKFCMQ CWSEYLTTKI MEEGMGQTIS CPAHGCDILV 

       250        260        270        280        290        300 
DDNTVMRLIT DSKVKLKYQH LITNSFVECN RLLKWCPAPD CHHVVKVQYP DAKPVRCKCG 

       310        320        330        340        350        360 
RQFCFNCGEN WHDPVKCKWL KKWIKKCDDD SETSNWIAAN TKECPKCHVT IEKDGGCNHM 

       370        380        390        400        410        420 
VCRNQNCKAE FCWVCLGPWE PHGSAWYNCN RYNEDDAKAA RDAQERSRAA LQRYLFYCNR 

       430        440        450        460        470        480 
YMNHMQSLRF EHKLYAQVKQ KMEEMQQHNM SWIEVQFLKK AVDVLCQCRA TLMYTYVFAF 

       490        500        510        520        530        540 
YLKKNNQSII FENNQADLEN ATEVLSGYLE RDISQDSLQD IKQKVQDKYR YCESRRRVLL 

       550 
QHVHEGYEKD LWEYIED

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The ubiquitin-conjugating enzymes UbcH7 and UbcH8 interact with RING finger/IBR motif-containing domains of HHARI and H7-AP1." Moynihan T.P., Ardley H.C., Nuber U., Rose S.A., Jones P.F., Markham A.F., Scheffner M., Robinson P.A. J. Biol. Chem. 274:30963-30968(1999) [PubMed: 10521492] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fetal brain.
[2]	Ardley H.C. Unpublished observations (MAY-2002) Cited for: SEQUENCE REVISION TO 227.
[3]	"Human ariadne homolog." Trockenbacher A., Marksteiner R., Schneider R. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"Ariadne-1: a vital Drosophila gene is required in development and defines a new conserved family of ring-finger proteins." Aguilera M., Oliveros M., Martinez-Padron M., Barbas J.A., Ferrus A. Genetics 155:1231-1244(2000) [PubMed: 10880484] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 95-557.
[6]	"Characterization of 16 novel human genes showing high similarity to yeast sequences." Stanchi F., Bertocco E., Toppo S., Dioguardi R., Simionati B., Cannata N., Zimbello R., Lanfranchi G., Valle G. Yeast 18:69-80(2001) [PubMed: 11124703] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 298-557. Tissue: Brain.
[7]	"Molecular and biological characterization of a new ring finger protein, MOP-6 which is highly expressed in activated human monocytes." Fujii Y., Takayama K., Ukai Y., Yoshimoto M. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 377-557. Tissue: Monocyte.
[8]	"Features of the parkin/ariadne-like ubiquitin ligase, HHARI, that regulate its interaction with the ubiquitin-conjugating enzyme, Ubch7." Ardley H.C., Tan N.G.S., Rose S.A., Markham A.F., Robinson P.A. J. Biol. Chem. 276:19640-19647(2001) [PubMed: 11278816] [Abstract] Cited for: INTERACTION WITH UBE2L3, MUTAGENESIS OF 187-GLN-ILE-188; ILE-188; CYS-208 AND TYR-258.

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Cross-references

Sequence databases

AJ243190 mRNA. Translation: CAB45870.1. Sequence problems.
AF072832 mRNA. Translation: AAD28088.1.
BC051877 mRNA. Translation: AAH51877.1.
AJ130976 mRNA. Translation: CAA10274.1.
AJ009771 mRNA. Translation: CAA08817.1.
AB014774 mRNA. Translation: BAB19786.1.

RefSeq

NP_005735.2.

UniGene

Hs.268787

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WD2	NMR	-	A	336-394	[»]

ModBase

Search...

Proteomic databases

PeptideAtlas

Q9Y4X5.

Genome annotation databases

Ensembl

ENSG00000166233. Homo sapiens. [Contig view]

GeneID

25820.

KEGG

hsa:25820.

Organism-specific databases

H-InvDB

HIX0012409.
HIX0022573.

HGNC

HGNC:689. ARIH1.

HPA

HPA003295.

MIM

605624. gene.

PharmGKB

PA24982.

GenAtlas

Search...

GeneCards

Search...

GeneLynx

Search...

Phylogenomic databases

HOGENOM

Q9Y4X5.

HOVERGEN

Q9Y4X5.

Gene expression databases

ArrayExpress

Q9Y4X5.

CleanEx

HS_ARIH1.

GermOnline

ENSG00000166233. Homo sapiens.

Family and domain databases

InterPro

IPR002867. Znf_C6HC.
IPR001841. Znf_RING.
[Graphical view]

Pfam

PF01485. IBR. 1 hit.
[Graphical view]

SMART

SM00647. IBR. 2 hits.
SM00184. RING. 2 hits.
[Graphical view]

PROSITE

PS00518. ZF_RING_1. False negative.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

ProDom

Q9Y4X5.
[Graphical view] [Entries sharing at least one domain]

BLOCKS

Search...

Other Resources

SOURCE

Search...

ProtoNet

Search...

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Entry information

Entry name

ARI1_HUMAN

Accession

Primary (citable) accession number: Q9Y4X5
Secondary accession number(s): O76026

Q9UP39

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	May 10, 2002
Last modified:	July 22, 2008
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents