Reviewed, UniProtKB/Swiss-Prot Q9Y6K8 (KAD5_HUMAN)

Names and origin

Protein names

Recommended name:
    Adenylate kinase isoenzyme 5
      Short name=AK 5
    EC=2.7.4.3
Alternative name(s):
    ATP-AMP transphosphorylase 5

Gene names

Name:

AK5

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Active on AMP and dAMP with ATP as a donor. When GTP is used as phosphate donor, the enzyme phosphorylates AMP, CMP, and to a small extent dCMP.
Catalytic activity	ATP + AMP = 2 ADP.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm.
Tissue specificity	Brain specific.
Sequence similarities	Belongs to the adenylate kinase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	ADP biosynthetic process Ref.1 Traceable author statement. Source: ProtInc dADP biosynthetic process Ref.1 Traceable author statement. Source: ProtInc pyrimidine ribonucleotide biosynthetic process Ref.1 Traceable author statement. Source: ProtInc
Cellular component	cytosol Ref.1 Traceable author statement. Source: ProtInc
Molecular function	adenylate kinase activity Ref.1 Traceable author statement. Source: ProtInc nucleoside kinase activity Ref.1 Inferred from Experiment. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Chain

1 – 198

198

Adenylate kinase isoenzyme 5

Regions

Nucleotide binding

18 – 26

9

ATP By similarity

Nucleotide binding

41 – 70

30

AMP By similarity

Sites

Active site

39

1

By similarity

Active site

96

1

By similarity

Experimental info

Sequence conflict

197

1

Missing in AAH12467. Ref.2

Secondary structure

1

.

.

.

.

.

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.

.

.

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.

.

.

.

.

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.

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198

Helix Strand Turn

Details...

Helix

4 – 10

7

Beta strand

13 – 18

6

Helix

24 – 35

12

Beta strand

38 – 41

4

Helix

42 – 52

11

Helix

55 – 65

11

Helix

72 – 86

15

Beta strand

93 – 96

4

Helix

102 – 111

10

Beta strand

116 – 122

7

Helix

125 – 134

10

Helix

142 – 170

29

Beta strand

171 – 177

7

Sequences

	Sequence		Length	Mass (Da)
	Q9Y6K8-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 8E8FA514CB7C3B58	FASTA	198	22,087
	10 20 30 40 50 60 MGGFMEDLRK CKIIFIIGGP GSGKGTQCEK LVEKYGFTHL STGELLREEL ASESERSKLI 70 80 90 100 110 120 RDIMERGDLV PSGIVLELLK EAMVASLGDT RGFLIDGYPR EVKQGEEFGR RIGDPQLVIC 130 140 150 160 170 180 MDCSADTMTN RLLQRSRSSL PVDDTTKTIA KRLEAYYRAS IPVIAYYETK TQLHKINAEG 190 TPEDVFLQLC TAIDSIIF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel human adenylate kinase. cDNA cloning, expression analysis, chromosome localization and characterization of the recombinant protein." Van Rompay A.R., Johansson M., Karlsson A. Eur. J. Biochem. 261:509-517(1999) [PubMed: 10215863] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF062595 mRNA. Translation: AAD27956.1.
BC012467 mRNA. Translation: AAH12467.2. Different initiation.

RefSeq

NP_036225.2.

UniGene

Hs.559718

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2BWJ	X-ray	2.30	A/B/C/D/E/F	1-196	[»]

ModBase

Search...

Genome annotation databases

Ensembl

ENSG00000154027. Homo sapiens. [Contig view]

GeneID

26289.

KEGG

hsa:26289.

Organism-specific databases

H-InvDB

HIX0020812.

HGNC

HGNC:365. AK5.

MIM

608009. gene.

PharmGKB

PA24659.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOVERGEN

Q9Y6K8.

Enzyme and pathway databases

Reactome

REACT_1698. Nucleotide metabolism.

Gene expression databases

ArrayExpress

Q9Y6K8.

CleanEx

HS_AK5.

GermOnline

ENSG00000154027. Homo sapiens.

Family and domain databases

InterPro

IPR000850. Adenylate_kin.
IPR006267. Adenylate_kin1.
[Graphical view]

PANTHER

PTHR23359. Adenylate_kin. 1 hit.

Pfam

PF00406. ADK. 1 hit.
[Graphical view]

PRINTS

PR00094. ADENYLTKNASE.

ProDom

PD000657. Adenylate_kin. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR01360. aden_kin_iso1. 1 hit.

PROSITE

PS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]

BLOCKS

Search...

Other Resources

SOURCE

Search...

ProtoNet

Search...

Entry information

Entry name

KAD5_HUMAN

Accession

Primary (citable) accession number: Q9Y6K8
Secondary accession number(s): Q96EC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1999
Last modified:	July 22, 2008
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)