Reviewed, UniProtKB/Swiss-Prot Q9Z0Z0 (KGP1B_MOUSE)

Names and origin

Protein names

Recommended name:
    cGMP-dependent protein kinase 1, beta isozyme
      Short name=cGK 1 beta
      Short name=cGKI-beta
    EC=2.7.11.12

Gene names

Name:	Prkg1
Synonyms:	Prkg1b, Prkgr1b

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	686 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Binding of cGMP results in enzyme activation By similarity.
Subunit structure	Antiparallel homodimer. Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1 By similarity. Interacts with MRVI1.
Miscellaneous	Exhibit a substrate specificity similar but not identical to that of CAK By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 2 cyclic nucleotide-binding domains. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding cGMP cGMP-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Gene Ontology (GO)
Biological process	dendrite development Inferred from mutant phenotype. Source: MGI forebrain development Inferred from mutant phenotype. Source: MGI neuron migration Inferred from mutant phenotype. Source: MGI protein amino acid phosphorylation Inferred from direct assay. Source: UniProtKB signal transduction Inferred from direct assay. Source: UniProtKB
Cellular component	Golgi apparatus Inferred from direct assay. Source: UniProtKB
Molecular function	cGMP binding Inferred from direct assay. Source: UniProtKB cGMP-dependent protein kinase activity Inferred from direct assay. Source: UniProtKB protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform CGK1-beta (identifier: Q9Z0Z0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform CGK1-alpha (identifier: P0C605-1)

The sequence of this isoform can be found in the external entry P0C605-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 686	686	cGMP-dependent protein kinase 1, beta isozyme
Regions
	Domain	375 – 634	260	Protein kinase
	Domain	635 – 686	52	AGC-kinase C-terminal
	Nucleotide binding	118 – 235	118	cGMP 1 By similarity
	Nucleotide binding	236 – 427	192	cGMP 2 By similarity
	Nucleotide binding	381 – 389	9	ATP By similarity
Sites
	Active site	499	1	Proton acceptor By similarity
	Binding site	405	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Isoform CGK1-beta [UniParc]. Last modified May 1, 1999. Version 1. Checksum: A0281F3530936016	FASTA	686	77,790
	10 20 30 40 50 60 MGTLRDLQYA LQEKIEELRQ RDALIDELEL ELDQKDELIQ KLQNELDKYR SVIRPATQQA 70 80 90 100 110 120 QKQSASTLQG EPRTKRQAIS AEPTAFDIQD LSHVTLPFYP KSPQSKDLIK EAILDNDFMK 130 140 150 160 170 180 NLELSQIQEI VDCMYPVEYG KDSCIIKEGD VGSLVYVMED GKVEVTKEGV KLCTMGPGKV 190 200 210 220 230 240 FGELAILYNC TRTATVKTLV NVKLWAIDRQ CFQTIMMRTG LIKHTEYMEF LKSVPTFQSL 250 260 270 280 290 300 PDEILSKLAD VLEETHYENG EYIIRQGARG DTFFIISKGQ VNVTREDSPS EDPVFLRTLG 310 320 330 340 350 360 KGDWFGEKAL QGEDVRTANV IAAEAVTCLV IDRDSFKHLI GGLDDVSNKA YEDAEAKAKY 370 380 390 400 410 420 EAEAAFFANL KLSDFNIIDT LGVGGFGRVE LVQLKSEESK TFAMKILKKR HIVDTRQQEH 430 440 450 460 470 480 IRSEKQIMQG AHSDFIVRLY RTFKDSKYLY MLMEACLGGE LWTILRDRGS FEDSTTRFYT 490 500 510 520 530 540 ACVVEAFAYL HSKGIIYRDL KPENLILDHR GYAKLVDFGF AKKIGFGKKT WTFCGTPEYV 550 560 570 580 590 600 APEIILNKGH DISADYWSLG ILMYELLTGS PPFSGPDPMK TYNIILRGID MIEFPKKIAK 610 620 630 640 650 660 NAANLIKKLC RDNPSERLGN LKNGVKDIQK HKWFEGFNWE GLRKGTLTPP IIPSVASPTD 670 680 TSNFDSFPED SDEPPPDDNS GWDIDF
	Isoform CGK1-alpha. See P0C605.		–	–

References

[1]	"Cyclic AMP- and cyclic GMP-dependent protein kinases differ in their regulation of cyclic AMP response element-dependent gene transcription." Collins S.P., Uhler M.D. J. Biol. Chem. 274:8391-8404(1999) [PubMed: 10085070] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Brain.
[2]	"IRAG mediates NO/cGMP-dependent inhibition of platelet aggregation and thrombus formation." Antl M., von Bruehl M.-L., Eiglsperger C., Werner M., Konrad I., Kocher T., Wilm M., Hofmann F., Massberg S., Schlossmann J. Blood 109:552-559(2007) [PubMed: 16990611] [Abstract] Cited for: INTERACTION WITH MRVI1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF084547 mRNA. Translation: AAD16044.1.
RefSeq	NP_035290.1.
UniGene	Mm.381170 Mm.381172
3D structure databases
HSSP	HSSP built from PDB template 1ATP based on UniProtKB P05132.
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000052920. Mus musculus. [Contig view]
GeneID	19091.
Organism-specific databases
MGI	MGI:108174. Prkg1.
Phylogenomic databases
HOGENOM	Q9Z0Z0.
HOVERGEN	Q9Z0Z0.
Gene expression databases
ArrayExpress	Q9Z0Z0.
CleanEx	MM_PRKG1.
GermOnline	ENSMUSG00000052920. Mus musculus.
Family and domain databases
InterPro	IPR002373. cAMP/cGMP_kin. IPR016232. cGMP-dependent_protein_kinase. IPR002374. cGMP_kinase_core. IPR000595. cNMP_bd. IPR000961. Pkinase_C. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR014710. RmlC-like_jellyroll. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 2 hits.
Pfam	PF00027. cNMP_binding. 2 hits. PF00069. Pkinase. 1 hit. [Graphical view]
PIRSF	PIRSF000559. cGMP-dep_kinase. 1 hit.
PRINTS	PR00103. CAMPKINASE. PR00104. CGMPKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00100. cNMP. 2 hits. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00888. CNMP_BINDING_1. 2 hits. PS00889. CNMP_BINDING_2. 2 hits. PS50042. CNMP_BINDING_3. 2 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

KGP1B_MOUSE

Accession

Primary (citable) accession number: Q9Z0Z0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	July 22, 2008
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)